Gene description for NRBP1
Gene name nuclear receptor binding protein 1
Gene symbol NRBP1
Other names/aliases BCON3
MADM
MUDPNP
NRBP
Species Homo sapiens
 Database cross references - NRBP1
ExoCarta ExoCarta_29959
Vesiclepedia VP_29959
Entrez Gene 29959
HGNC 7993
MIM 606010
UniProt Q9UHY1  
 NRBP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Mesenchymal stem cells 36408942    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for NRBP1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein homodimerization activity GO:0042803 ISS
Biological Process
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IDA
    DNA damage response GO:0006974 IBA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cell cortex GO:0005938 IEA
    endomembrane system GO:0012505 IDA
    membrane GO:0016020 IEA
    lamellipodium GO:0030027 IEA
 Experiment description of studies that identified NRBP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NRBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KIF2A 3796
Co-fractionation Homo sapiens
2 SFN 2810
Co-fractionation Homo sapiens
3 SNX1 6642
Affinity Capture-MS Homo sapiens
4 RIT1 6016
Negative Genetic Homo sapiens
5 LHX4  
Affinity Capture-MS Homo sapiens
6 TBC1D4  
Affinity Capture-MS Homo sapiens
7 MAP2K2 5605
Affinity Capture-MS Homo sapiens
8 ACOT9 23597
Affinity Capture-MS Homo sapiens
9 FASN 2194
Positive Genetic Homo sapiens
Negative Genetic Homo sapiens
10 C2orf50  
Two-hybrid Homo sapiens
11 CSNK2B 1460
Two-hybrid Homo sapiens
12 TCEB1 6921
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 LRPPRC 10128
Co-fractionation Homo sapiens
14 TK1 7083
Two-hybrid Homo sapiens
15 GSK3B 2932
Two-hybrid Homo sapiens
16 LRIF1  
Two-hybrid Homo sapiens
17 HSPA8 3312
Affinity Capture-MS Homo sapiens
18 TSEN15 116461
Affinity Capture-MS Homo sapiens
19 HARS2 23438
Affinity Capture-MS Homo sapiens
20 YWHAB 7529
Co-fractionation Homo sapiens
21 YWHAQ 10971
Co-fractionation Homo sapiens
22 DHX15 1665
Co-fractionation Homo sapiens
23 PSMD11 5717
Co-fractionation Homo sapiens
24 GADD45A  
Two-hybrid Homo sapiens
25 BBS7 55212
Affinity Capture-MS Homo sapiens
26 PPP1CC 5501
Two-hybrid Homo sapiens
27 RBM25 58517
Affinity Capture-MS Homo sapiens
28 CD2AP 23607
Co-fractionation Homo sapiens
29 MLF1  
Affinity Capture-MS Homo sapiens
30 NME3 4832
Affinity Capture-MS Homo sapiens
31 KIAA1586  
Affinity Capture-MS Homo sapiens
32 SLC25A15  
Affinity Capture-MS Homo sapiens
33 LOC254896  
Affinity Capture-MS Homo sapiens
34 SNX31  
Two-hybrid Homo sapiens
35 CDKN1A  
Two-hybrid Homo sapiens
36 PARK2  
Affinity Capture-MS Homo sapiens
37 FMN2  
Affinity Capture-MS Homo sapiens
38 TTF2 8458
Affinity Capture-MS Homo sapiens
39 PDPK1 5170
Affinity Capture-MS Homo sapiens
40 DDX58 23586
Affinity Capture-RNA Homo sapiens
41 SNX6 58533
Affinity Capture-MS Homo sapiens
42 ELP2 55250
Co-fractionation Homo sapiens
43 ADAM19 8728
Affinity Capture-MS Homo sapiens
44 DDX19B 11269
Affinity Capture-MS Homo sapiens
45 BPGM 669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 TGM2 7052
Co-fractionation Homo sapiens
47 RPE  
Co-fractionation Homo sapiens
48 COPS5 10987
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
49 RNH1 6050
Co-fractionation Homo sapiens
50 CAPZB 832
Affinity Capture-MS Homo sapiens
51 PRKACA 5566
Proximity Label-MS Homo sapiens
52 SORBS3 10174
Two-hybrid Homo sapiens
53 TSC22D4 81628
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
54 TSC22D1 8848
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 PPP2R1A 5518
Co-fractionation Homo sapiens
56 TCEB2 6923
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 MYH9 4627
Co-fractionation Homo sapiens
58 TRIM16 10626
Co-fractionation Homo sapiens
59 DNAJB4 11080
Proximity Label-MS Homo sapiens
60 FAM117B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 SAT2 112483
Affinity Capture-MS Homo sapiens
62 GNS 2799
Co-fractionation Homo sapiens
63 KIF5B 3799
Co-fractionation Homo sapiens
64 DNA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CA10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 SART3 9733
Co-fractionation Homo sapiens
67 RIBC2  
Two-hybrid Homo sapiens
68 TSC22D2 9819
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 KLK9  
Affinity Capture-MS Homo sapiens
70 SNX2 6643
Affinity Capture-MS Homo sapiens
71 KRAS 3845
Negative Genetic Homo sapiens
72 CTPS1 1503
Co-fractionation Homo sapiens
73 ABHD17B 51104
Affinity Capture-MS Homo sapiens
74 SS18L2  
Proximity Label-MS Homo sapiens
75 SPANXN2  
Affinity Capture-MS Homo sapiens
76 HAT1 8520
Co-fractionation Homo sapiens
77 TSC22D3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 LENG8  
Affinity Capture-MS Homo sapiens
79 LANCL1 10314
Affinity Capture-MS Homo sapiens
80 PLXDC2 84898
Affinity Capture-MS Homo sapiens
81 DSE 29940
Affinity Capture-MS Homo sapiens
82 NRBP2  
Affinity Capture-MS Homo sapiens
83 PSMD3 5709
Affinity Capture-MS Homo sapiens
84 C1QL3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here