Gene description for KIF5A
Gene name kinesin family member 5A
Gene symbol KIF5A
Other names/aliases D12S1889
MY050
NKHC
SPG10
Species Homo sapiens
 Database cross references - KIF5A
ExoCarta ExoCarta_3798
Vesiclepedia VP_3798
Entrez Gene 3798
HGNC 6323
MIM 602821
UniProt Q12840  
 KIF5A identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for KIF5A
Molecular Function
    cytoskeletal motor activity GO:0003774 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    plus-end-directed microtubule motor activity GO:0008574 IBA
    ATP hydrolysis activity GO:0016887 IBA
    kinesin binding GO:0019894 IEA
Biological Process
    microtubule-based movement GO:0007018 TAS
    chemical synaptic transmission GO:0007268 TAS
    axon guidance GO:0007411 IBA
    vesicle-mediated transport GO:0016192 ISS
    synaptic vesicle transport GO:0048489 IBA
    anterograde dendritic transport of neurotransmitter receptor complex GO:0098971 IBA
    anterograde axonal protein transport GO:0099641 ISS
    retrograde neuronal dense core vesicle transport GO:1990049 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    membrane GO:0016020 HDA
    dendrite cytoplasm GO:0032839 IEA
    ciliary rootlet GO:0035253 IEA
    perikaryon GO:0043204 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    postsynaptic cytosol GO:0099524 IEA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified KIF5A in exosomes
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KIF5A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 YWHAE 7531
Affinity Capture-MS Homo sapiens
3 TRIP6 7205
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 KRT37 8688
Affinity Capture-MS Homo sapiens
5 TRAK2 66008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 TP53BP2  
Two-hybrid Homo sapiens
7 BRK1 55845
Affinity Capture-MS Homo sapiens
8 HSP90B2P  
Cross-Linking-MS (XL-MS) Homo sapiens
9 TSG101 7251
Two-hybrid Homo sapiens
10 SMN1 6606
Two-hybrid Homo sapiens
11 ITSN1 6453
Two-hybrid Homo sapiens
12 MAGEA3  
Affinity Capture-MS Homo sapiens
13 TK1 7083
Two-hybrid Homo sapiens
14 NUP62CL  
Affinity Capture-MS Homo sapiens
15 RABEP2 79874
Affinity Capture-MS Homo sapiens
16 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 ANAPC2 29882
Proximity Label-MS Homo sapiens
18 WHAMMP3  
Affinity Capture-MS Homo sapiens
19 PIN1 5300
Two-hybrid Homo sapiens
20 KLC3  
Affinity Capture-MS Homo sapiens
21 NUP62 23636
Affinity Capture-MS Homo sapiens
22 S100A2 6273
Affinity Capture-MS Homo sapiens
23 HIST1H2BH 8345
Cross-Linking-MS (XL-MS) Homo sapiens
24 INSR 3643
Affinity Capture-Western Homo sapiens
25 SPSB4  
Affinity Capture-MS Homo sapiens
26 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 NPEPPS 9520
Affinity Capture-MS Homo sapiens
28 MAP4K4 9448
Two-hybrid Homo sapiens
29 CEBPG  
Affinity Capture-MS Homo sapiens
30 TRIM55  
Two-hybrid Homo sapiens
31 KLC1 3831
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
32 C15orf59  
Affinity Capture-MS Homo sapiens
33 KIF5B 3799
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
34 HSP90B1 7184
Cross-Linking-MS (XL-MS) Homo sapiens
35 KLC4 89953
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
36 STAU1 6780
Affinity Capture-Western Homo sapiens
37 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CREBBP  
Two-hybrid Homo sapiens
39 KCNE3  
Two-hybrid Homo sapiens
40 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 SYNE4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 CHRNA9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 RAPGEF2 9693
Two-hybrid Homo sapiens
44 KRT38 8687
Affinity Capture-MS Homo sapiens
45 YAP1 10413
Two-hybrid Homo sapiens
46 DTNB 1838
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
47 EXOC1 55763
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 KLC2 64837
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
49 KXD1 79036
Affinity Capture-MS Homo sapiens
50 NDEL1 81565
Two-hybrid Homo sapiens
51 CCDC14  
Proximity Label-MS Homo sapiens
52 KIF11 3832
Co-fractionation Homo sapiens
53 YWHAQ 10971
Affinity Capture-MS Homo sapiens
54 KIF5C 3800
Cross-Linking-MS (XL-MS) Homo sapiens
55 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 DYNLL2 140735
Affinity Capture-MS Homo sapiens
57 BMP1 649
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 VPS26B 112936
Affinity Capture-MS Homo sapiens
59 MYCL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 DYNLL1 8655
Affinity Capture-MS Homo sapiens
61 TRAK1 22906
Affinity Capture-MS Homo sapiens
62 TRIM63  
Two-hybrid Homo sapiens
63 SYCE1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here