Gene description for Snrnp70
Gene name small nuclear ribonucleoprotein 70 (U1)
Gene symbol Snrnp70
Other names/aliases 2700022N21Rik
3200002N22Rik
AI325098
R74807
Rnu1p70
Rnulp70
Snrp70
Srnp70
U1-70
U1-70K
Species Mus musculus
 Database cross references - Snrnp70
ExoCarta ExoCarta_20637
Vesiclepedia VP_20637
Entrez Gene 20637
UniProt Q62376  
 Snrnp70 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Snrnp70
Molecular Function
    RNA binding GO:0003723 ISO
    RNA binding GO:0003723 ISS
    mRNA binding GO:0003729 IBA
    U1 snRNA binding GO:0030619 IBA
    U1 snRNA binding GO:0030619 ISO
    U1 snRNA binding GO:0030619 ISS
    U1 snRNP binding GO:1990446 ISO
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    regulation of RNA splicing GO:0043484 ISO
    regulation of RNA splicing GO:0043484 ISS
    positive regulation of mRNA splicing, via spliceosome GO:0048026 IMP
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 ISO
    spliceosomal complex GO:0005681 ISS
    U1 snRNP GO:0005685 IBA
    U1 snRNP GO:0005685 ISO
    U1 snRNP GO:0005685 ISS
    nuclear speck GO:0016607 IDA
    U2-type prespliceosome GO:0071004 IBA
 Experiment description of studies that identified Snrnp70 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Snrnp70
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GEMIN6  
Affinity Capture-MS Homo sapiens
2 ZNF668  
Affinity Capture-MS Homo sapiens
3 PKN3 29941
Affinity Capture-MS Homo sapiens
4 SNRPG 6637
Affinity Capture-MS Homo sapiens
5 TOE1 114034
Affinity Capture-MS Homo sapiens
6 SMN1 6606
Affinity Capture-MS Homo sapiens
7 Prpf4b 19134
Co-fractionation Mus musculus
8 ABT1 29777
Affinity Capture-MS Homo sapiens
9 GEMIN2 8487
Affinity Capture-MS Homo sapiens
10 1110037F02Rik 66185
Co-fractionation Mus musculus
11 KLHL8  
Affinity Capture-MS Homo sapiens
12 Bcas2  
Co-fractionation Mus musculus
13 SNRPB 6628
Affinity Capture-MS Homo sapiens
14 GEMIN5 25929
Affinity Capture-MS Homo sapiens
15 Srsf7 225027
Co-fractionation Mus musculus
16 Atxn1  
Affinity Capture-MS Mus musculus
17 Foxp3  
Affinity Capture-MS Mus musculus
18 Cebpa  
Affinity Capture-MS Mus musculus
19 RPRD2  
Affinity Capture-MS Homo sapiens
20 Snrnp40  
Co-fractionation Mus musculus
21 Smu1  
Co-fractionation Mus musculus
22 GEMIN8 54960
Affinity Capture-MS Homo sapiens
23 Ik  
Co-fractionation Mus musculus
24 Cwc22  
Co-fractionation Mus musculus
25 LSM11  
Affinity Capture-MS Homo sapiens
26 PHAX  
Affinity Capture-MS Homo sapiens
27 SNRPF 6636
Affinity Capture-MS Homo sapiens
28 SNRPD2 6633
Affinity Capture-MS Homo sapiens
29 Mov10 17454
Co-fractionation Mus musculus
30 APOBEC3B 9582
Affinity Capture-MS Homo sapiens
31 STRAP 11171
Affinity Capture-MS Homo sapiens
32 TXN 7295
Affinity Capture-MS Homo sapiens
33 CCNB1 891
Two-hybrid Homo sapiens
34 DDX20 11218
Affinity Capture-MS Homo sapiens
35 SNRPD1 6632
Affinity Capture-MS Homo sapiens
36 Tfip11  
Co-fractionation Mus musculus
37 Cdk12  
Co-fractionation Mus musculus
38 Snrpc 20630
Co-fractionation Mus musculus
39 Htatsf1  
Co-fractionation Mus musculus
40 ZNF503 84858
Affinity Capture-MS Homo sapiens
41 Hnrnpm 76936
Co-fractionation Mus musculus
42 Dhx8  
Co-fractionation Mus musculus
43 TAF15 8148
Affinity Capture-MS Homo sapiens
44 SNRPC 6631
Affinity Capture-MS Homo sapiens
45 GEMIN7  
Affinity Capture-MS Homo sapiens
46 Cactin  
Co-fractionation Mus musculus
47 CAPN1 823
Affinity Capture-MS Homo sapiens
48 Tia1  
Affinity Capture-MS Mus musculus
49 Rbm25  
Co-fractionation Mus musculus
50 GEMIN4 50628
Affinity Capture-MS Homo sapiens
51 SNRPD3 6634
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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