Gene description for COG4
Gene name component of oligomeric golgi complex 4
Gene symbol COG4
Other names/aliases CDG2J
COD1
Species Homo sapiens
 Database cross references - COG4
ExoCarta ExoCarta_25839
Vesiclepedia VP_25839
Entrez Gene 25839
HGNC 18620
MIM 606976
UniProt Q9H9E3  
 COG4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for COG4
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
Biological Process
    retrograde transport, vesicle recycling within Golgi GO:0000301 IMP
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IMP
    Golgi organization GO:0007030 IBA
    Golgi organization GO:0007030 IMP
    protein transport GO:0015031 IEA
    glycosylation GO:0070085 IMP
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    Golgi membrane GO:0000139 TAS
    cytosol GO:0005829 IEA
    Golgi transport complex GO:0017119 IBA
    Golgi transport complex GO:0017119 IDA
    Golgi transport complex GO:0017119 NAS
    trans-Golgi network membrane GO:0032588 TAS
 Experiment description of studies that identified COG4 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for COG4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GJD3  
Proximity Label-MS Homo sapiens
2 METTL7A 25840
Proximity Label-MS Homo sapiens
3 APC  
Two-hybrid Homo sapiens
4 FPR1  
Affinity Capture-MS Homo sapiens
5 COG2 22796
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
6 SEPT2 4735
Two-hybrid Homo sapiens
7 NDUFS2 4720
Co-fractionation Homo sapiens
8 RPS20 6224
Co-fractionation Homo sapiens
9 PSMC5 5705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 KRT8 3856
Proximity Label-MS Homo sapiens
11 CHP1 11261
Affinity Capture-MS Homo sapiens
12 OPRM1 4988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ZGPAT  
Affinity Capture-MS Homo sapiens
14 SEMA4C 54910
Affinity Capture-MS Homo sapiens
15 ITFG3 83986
Affinity Capture-MS Homo sapiens
16 F2RL1  
Affinity Capture-MS Homo sapiens
17 MECP2 4204
Affinity Capture-MS Homo sapiens
18 CA14 23632
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 EBAG9 9166
Proximity Label-MS Homo sapiens
20 GPR17 2840
Affinity Capture-MS Homo sapiens
21 LAMTOR1 55004
Proximity Label-MS Homo sapiens
22 MTNR1B  
Two-hybrid Homo sapiens
23 NUP155 9631
Proximity Label-MS Homo sapiens
24 BSG 682
Affinity Capture-MS Homo sapiens
25 PDK1 5163
Affinity Capture-MS Homo sapiens
26 VSIG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 BEX2  
Two-hybrid Homo sapiens
28 GCGR  
Affinity Capture-MS Homo sapiens
29 CUL4B 8450
Affinity Capture-MS Homo sapiens
30 FAM110A  
Two-hybrid Homo sapiens
31 P2RY8  
Affinity Capture-MS Homo sapiens
32 SMEK1 55671
Affinity Capture-MS Homo sapiens
33 ILVBL 10994
Affinity Capture-MS Homo sapiens
34 COMTD1 118881
Affinity Capture-MS Homo sapiens
35 RAB30 27314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 VCP 7415
Affinity Capture-MS Homo sapiens
37 NTRK1 4914
Affinity Capture-MS Homo sapiens
38 GPR182  
Affinity Capture-MS Homo sapiens
39 PEX14 5195
Proximity Label-MS Homo sapiens
40 COG3 83548
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 CTNNA3  
Two-hybrid Homo sapiens
42 COG5 10466
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
43 ELOVL5 60481
Proximity Label-MS Homo sapiens
44 TMCO3 55002
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 SLC2A9  
Affinity Capture-MS Homo sapiens
46 CCDC59  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 CD40 958
Affinity Capture-MS Homo sapiens
48 VIPR2  
Affinity Capture-MS Homo sapiens
49 EFNA4  
Affinity Capture-MS Homo sapiens
50 APLNR  
Affinity Capture-MS Homo sapiens
51 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 COMMD8 54951
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 NPTN 27020
Affinity Capture-MS Homo sapiens
54 TEKT4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 CD274 29126
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 HSD3B7 80270
Proximity Label-MS Homo sapiens
57 NPY2R  
Affinity Capture-MS Homo sapiens
58 EGFR 1956
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
59 TXNDC9 10190
Two-hybrid Homo sapiens
60 COG1 9382
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
61 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
62 MUL1  
Affinity Capture-MS Homo sapiens
63 RPA3 6119
Proximity Label-MS Homo sapiens
64 COG8 84342
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
65 GPR45  
Affinity Capture-MS Homo sapiens
66 RAB9A 9367
Proximity Label-MS Homo sapiens
67 SORBS3 10174
Two-hybrid Homo sapiens
68 SCN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 GYPB  
Affinity Capture-MS Homo sapiens
70 NUP54 53371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
72 OCIAD1 54940
Affinity Capture-MS Homo sapiens
73 TRIM45  
Affinity Capture-MS Homo sapiens
74 MFSD4  
Affinity Capture-MS Homo sapiens
75 CHRM4  
Affinity Capture-MS Homo sapiens
76 HDAC1 3065
Affinity Capture-MS Homo sapiens
77 C15orf59  
Affinity Capture-MS Homo sapiens
78 PSMA1 5682
Two-hybrid Homo sapiens
79 VSIG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 FARSA 2193
Two-hybrid Homo sapiens
81 RAB7A 7879
Proximity Label-MS Homo sapiens
82 ANKRD11 29123
Two-hybrid Homo sapiens
83 SERBP1 26135
Affinity Capture-MS Homo sapiens
84 KRAS 3845
Negative Genetic Homo sapiens
85 CCDC96  
Affinity Capture-MS Homo sapiens
86 CCDC40  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 COG7 91949
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
88 CAV1 857
Proximity Label-MS Homo sapiens
89 CUL4A 8451
Affinity Capture-MS Homo sapiens
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