Gene description for ATP13A1
Gene name ATPase type 13A1
Gene symbol ATP13A1
Other names/aliases ATP13A
CGI-152
Species Homo sapiens
 Database cross references - ATP13A1
ExoCarta ExoCarta_57130
Vesiclepedia VP_57130
Entrez Gene 57130
HGNC 24215
UniProt Q9HD20  
 ATP13A1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ATP13A1
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ABC-type manganese transporter activity GO:0015410 TAS
    P-type ion transporter activity GO:0015662 IBA
    ATP hydrolysis activity GO:0016887 IEA
    ATPase-coupled monoatomic cation transmembrane transporter activity GO:0019829 IBA
    metal ion binding GO:0046872 IEA
    membrane protein dislocase activity GO:0140567 IBA
    membrane protein dislocase activity GO:0140567 IDA
Biological Process
    intracellular calcium ion homeostasis GO:0006874 IBA
    protein transport GO:0015031 IEA
    monoatomic ion transmembrane transport GO:0034220 TAS
    transmembrane transport GO:0055085 IBA
    manganese ion transmembrane transport GO:0071421 IEA
    extraction of mislocalized protein from ER membrane GO:0140569 IBA
    extraction of mislocalized protein from ER membrane GO:0140569 IDA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified ATP13A1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ATP13A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGOLN2 10618
Affinity Capture-MS Homo sapiens
2 TPRA1  
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 HSD17B11 51170
Proximity Label-MS Homo sapiens
5 ISG15 9636
Affinity Capture-MS Homo sapiens
6 TMBIM6 7009
Two-hybrid Homo sapiens
7 COIL  
Proximity Label-MS Homo sapiens
8 METTL7A 25840
Proximity Label-MS Homo sapiens
9 LAMP3  
Proximity Label-MS Homo sapiens
10 SYNGR1 9145
Affinity Capture-MS Homo sapiens
11 CDH5  
Affinity Capture-MS Homo sapiens
12 GLP1R  
PCA Homo sapiens
Affinity Capture-Western Homo sapiens
13 TYROBP  
Affinity Capture-MS Homo sapiens
14 GJD3  
Proximity Label-MS Homo sapiens
15 LAMP2 3920
Proximity Label-MS Homo sapiens
16 DNAJC25 548645
Proximity Label-MS Homo sapiens
17 SLC7A14  
Affinity Capture-MS Homo sapiens
18 HEPACAM2  
Affinity Capture-MS Homo sapiens
19 APEX1 328
Affinity Capture-RNA Homo sapiens
20 GABRG2  
Affinity Capture-MS Homo sapiens
21 BTN2A1 11120
Affinity Capture-MS Homo sapiens
22 C4orf32  
Affinity Capture-MS Homo sapiens
23 SLC30A8  
Two-hybrid Homo sapiens
24 TMEM130  
Affinity Capture-MS Homo sapiens
25 MMD  
Two-hybrid Homo sapiens
26 SLC10A6  
Two-hybrid Homo sapiens
27 SSR1 6745
Proximity Label-MS Homo sapiens
28 GPBAR1  
Affinity Capture-MS Homo sapiens
29 PIGH  
Affinity Capture-MS Homo sapiens
30 AGTR1  
Two-hybrid Homo sapiens
31 PGRMC2 10424
Affinity Capture-MS Homo sapiens
32 C3orf52  
Affinity Capture-MS Homo sapiens
33 MANBAL 63905
Two-hybrid Homo sapiens
34 HIATL1 84641
Two-hybrid Homo sapiens
35 CCDC47 57003
Affinity Capture-MS Homo sapiens
36 DDX6 1656
Affinity Capture-MS Homo sapiens
37 BUD31 8896
Two-hybrid Homo sapiens
38 MARCKS 4082
Proximity Label-MS Homo sapiens
39 NUP155 9631
Proximity Label-MS Homo sapiens
40 PXMP2  
Proximity Label-MS Homo sapiens
41 TMEM14B  
Two-hybrid Homo sapiens
42 EMD 2010
Proximity Label-MS Homo sapiens
43 RB1CC1 9821
Affinity Capture-MS Homo sapiens
44 KIAA1644  
Affinity Capture-MS Homo sapiens
45 IL1R2  
Affinity Capture-MS Homo sapiens
46 VSIG4  
Affinity Capture-MS Homo sapiens
47 LRRC25  
Affinity Capture-MS Homo sapiens
48 SYPL2  
Two-hybrid Homo sapiens
49 C12orf49  
Affinity Capture-MS Homo sapiens
50 CXCR2  
Two-hybrid Homo sapiens
51 PSMD2 5708
Affinity Capture-MS Homo sapiens
52 PCDHGC4  
Affinity Capture-MS Homo sapiens
53 TRIM21 6737
Affinity Capture-MS Homo sapiens
54 NTRK1 4914
Affinity Capture-MS Homo sapiens
55 UNC93A  
Two-hybrid Homo sapiens
56 CCDC107  
Affinity Capture-MS Homo sapiens
57 RPN1 6184
Proximity Label-MS Homo sapiens
58 TMEM56  
Two-hybrid Homo sapiens
59 VSTM1  
Affinity Capture-MS Homo sapiens
60 SCN3B  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
61 ELOVL5 60481
Proximity Label-MS Homo sapiens
62 TMEM206 55248
Affinity Capture-MS Homo sapiens
63 SEC61B 10952
Proximity Label-MS Homo sapiens
64 REEP5 7905
Proximity Label-MS Homo sapiens
65 SEC63 11231
Proximity Label-MS Homo sapiens
66 CD40 958
Affinity Capture-MS Homo sapiens
67 SLC30A2  
Two-hybrid Homo sapiens
68 RNASEK  
Two-hybrid Homo sapiens
69 RPN2 6185
Proximity Label-MS Homo sapiens
70 CANX 821
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
71 FFAR2  
Two-hybrid Homo sapiens
72 PANX1 24145
Proximity Label-MS Homo sapiens
73 CD83  
Affinity Capture-MS Homo sapiens
74 CD300LB  
Affinity Capture-MS Homo sapiens
75 LAMP1 3916
Proximity Label-MS Homo sapiens
76 MARCH8  
Affinity Capture-MS Homo sapiens
77 LRRTM1 347730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 BCAP31 10134
Proximity Label-MS Homo sapiens
79 HSD3B7 80270
Proximity Label-MS Homo sapiens
80 TNFRSF9  
Affinity Capture-MS Homo sapiens
81 NIPAL1  
Affinity Capture-MS Homo sapiens
82 SLC16A13 201232
Two-hybrid Homo sapiens
83 ARF6 382
Proximity Label-MS Homo sapiens
84 CD226 10666
Affinity Capture-MS Homo sapiens
85 CD27  
Affinity Capture-MS Homo sapiens
86 FAM209A 200232
Two-hybrid Homo sapiens
87 GPR42  
Two-hybrid Homo sapiens
88 C3orf18  
Affinity Capture-MS Homo sapiens
89 TMEM237 65062
Two-hybrid Homo sapiens
90 RPA3 6119
Proximity Label-MS Homo sapiens
91 ATP2A1 487
Proximity Label-MS Homo sapiens
92 ARHGEF5  
Two-hybrid Homo sapiens
93 KITLG  
Affinity Capture-MS Homo sapiens
94 HLA-F 3134
Affinity Capture-MS Homo sapiens
95 CD244 51744
Affinity Capture-MS Homo sapiens
96 TGFA  
Affinity Capture-MS Homo sapiens
97 ENTPD2 954
Affinity Capture-MS Homo sapiens
98 CYP2C9  
Proximity Label-MS Homo sapiens
99 PGRMC1 10857
Affinity Capture-MS Homo sapiens
100 LMNA 4000
Proximity Label-MS Homo sapiens
101 PTAFR  
Affinity Capture-MS Homo sapiens
102 VMA21  
Two-hybrid Homo sapiens
103 TSPAN10 83882
Affinity Capture-MS Homo sapiens
104 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
105 FASN 2194
Negative Genetic Homo sapiens
106 TRIM13  
Affinity Capture-MS Homo sapiens
107 PARP1 142
Proximity Label-MS Homo sapiens
108 TXNDC15  
Affinity Capture-MS Homo sapiens
109 OPALIN  
Affinity Capture-MS Homo sapiens
110 LDLRAD1  
Affinity Capture-MS Homo sapiens
111 ASIC4  
Affinity Capture-MS Homo sapiens
112 VCPIP1 80124
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 TSPAN15 23555
Affinity Capture-MS Homo sapiens
114 EBP  
Two-hybrid Homo sapiens
115 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 ZYG11B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
117 NUP35 129401
Proximity Label-MS Homo sapiens
118 ERGIC1 57222
Proximity Label-MS Homo sapiens
119 DDX58 23586
Affinity Capture-RNA Homo sapiens
120 C1orf186  
Affinity Capture-MS Homo sapiens
121 SEC62 7095
Proximity Label-MS Homo sapiens
122 DIO3  
Affinity Capture-MS Homo sapiens
123 LPAR2 9170
Affinity Capture-MS Homo sapiens
124 TPST2 8459
Affinity Capture-MS Homo sapiens
125 EVA1B 55194
Affinity Capture-MS Homo sapiens
126 KIR2DL4  
Affinity Capture-MS Homo sapiens
127 RAB5C 5878
Proximity Label-MS Homo sapiens
128 ADGRE5 976
Affinity Capture-MS Homo sapiens
129 CCR6  
Affinity Capture-MS Homo sapiens
130 ELOVL4  
Two-hybrid Homo sapiens
131 KCNE4  
Affinity Capture-MS Homo sapiens
132 CXCL16 58191
Affinity Capture-MS Homo sapiens
133 DERL1 79139
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ATP13A1 is involved
PathwayEvidenceSource
Ion channel transport TAS Reactome
Ion transport by P-type ATPases TAS Reactome
Transport of small molecules TAS Reactome





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