Gene description for COG3
Gene name component of oligomeric golgi complex 3
Gene symbol COG3
Other names/aliases SEC34
Species Homo sapiens
 Database cross references - COG3
ExoCarta ExoCarta_83548
Vesiclepedia VP_83548
Entrez Gene 83548
HGNC 18619
MIM 606975
UniProt Q96JB2  
 COG3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for COG3
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    retrograde transport, vesicle recycling within Golgi GO:0000301 IMP
    protein glycosylation GO:0006486 IMP
    intracellular protein transport GO:0006886 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IDA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IMP
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    intra-Golgi vesicle-mediated transport GO:0006891 IDA
    Golgi organization GO:0007030 IBA
    Golgi organization GO:0007030 IGI
    Golgi organization GO:0007030 IMP
    protein localization to organelle GO:0033365 IMP
    protein stabilization GO:0050821 IMP
    glycosylation GO:0070085 IMP
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IDA
    cis-Golgi network GO:0005801 IEA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    Golgi transport complex GO:0017119 IBA
    Golgi transport complex GO:0017119 IDA
    Golgi transport complex GO:0017119 NAS
    Golgi cisterna membrane GO:0032580 IEA
    trans-Golgi network membrane GO:0032588 TAS
 Experiment description of studies that identified COG3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for COG3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AKAP10 11216
Co-fractionation Homo sapiens
2 CDC73  
Co-fractionation Homo sapiens
3 TRIM2  
Two-hybrid Homo sapiens
4 GJD3  
Proximity Label-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 ILVBL 10994
Affinity Capture-MS Homo sapiens
7 CLIC1 1192
Co-fractionation Homo sapiens
8 CTR9 9646
Co-fractionation Homo sapiens
9 EBAG9 9166
Proximity Label-MS Homo sapiens
10 NUPL1 9818
Two-hybrid Homo sapiens
11 TSSC1 7260
Synthetic Lethality Homo sapiens
12 HAUS4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 GPR17 2840
Affinity Capture-MS Homo sapiens
14 C10orf76  
Synthetic Lethality Homo sapiens
15 CINP  
Two-hybrid Homo sapiens
16 RAB5A 5868
Proximity Label-MS Homo sapiens
17 RAB1B 81876
Synthetic Lethality Homo sapiens
Affinity Capture-MS Homo sapiens
18 CMTM8  
Affinity Capture-MS Homo sapiens
19 TBCC 6903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 COMMD8 54951
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 TSHZ2  
Two-hybrid Homo sapiens
23 ZDHHC17 23390
Two-hybrid Homo sapiens
24 MYH14 79784
Co-fractionation Homo sapiens
25 GPR182  
Affinity Capture-MS Homo sapiens
26 CD274 29126
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CHRM4  
Affinity Capture-MS Homo sapiens
28 COG8 84342
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 FPR1  
Affinity Capture-MS Homo sapiens
30 GCGR  
Affinity Capture-MS Homo sapiens
31 CD40 958
Affinity Capture-MS Homo sapiens
32 EGFR 1956
Negative Genetic Homo sapiens
33 COG2 22796
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 ARF6 382
Proximity Label-MS Homo sapiens
35 RABL6 55684
Co-fractionation Homo sapiens
36 DCTN2 10540
Two-hybrid Homo sapiens
37 PSMA6 5687
Affinity Capture-MS Homo sapiens
38 HTR6  
Affinity Capture-MS Homo sapiens
39 OCIAD1 54940
Affinity Capture-MS Homo sapiens
40 COG6 57511
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 COG1 9382
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 CEP250 11190
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 COMTD1 118881
Affinity Capture-MS Homo sapiens
44 ABI2 10152
Two-hybrid Homo sapiens
45 VSIG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 UXS1 80146
Affinity Capture-MS Homo sapiens
47 KIFC3 3801
Two-hybrid Homo sapiens
48 CMAS 55907
Affinity Capture-MS Homo sapiens
49 RAB30 27314
Affinity Capture-MS Homo sapiens
50 HAUS1  
Affinity Capture-MS Homo sapiens
51 EFNA4  
Affinity Capture-MS Homo sapiens
52 PMVK 10654
Affinity Capture-MS Homo sapiens
53 NTRK1 4914
Affinity Capture-MS Homo sapiens
54 GPR45  
Affinity Capture-MS Homo sapiens
55 NPTN 27020
Affinity Capture-MS Homo sapiens
56 ACTR3 10096
Co-fractionation Homo sapiens
57 GOLGA1  
Proximity Label-MS Homo sapiens
58 SEC24C 9632
Co-fractionation Homo sapiens
59 PEX14 5195
Proximity Label-MS Homo sapiens
60 RAB7A 7879
Proximity Label-MS Homo sapiens
61 SCN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 OPRM1 4988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 KRAS 3845
Negative Genetic Homo sapiens
64 ACTR1A 10121
Co-fractionation Homo sapiens
65 P2RY10  
Affinity Capture-MS Homo sapiens
66 AP3B1 8546
Co-fractionation Homo sapiens
67 COG4 25839
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 COG5 10466
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
69 COG7 91949
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 CAV1 857
Proximity Label-MS Homo sapiens
71 MYL12A 10627
Co-fractionation Homo sapiens
72 KRT7 3855
Co-fractionation Homo sapiens
73 RAB1A 5861
Synthetic Lethality Homo sapiens
74 HAUS3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 TACSTD2 4070
Affinity Capture-MS Homo sapiens
76 ITFG3 83986
Affinity Capture-MS Homo sapiens
77 PTAR1 375743
Synthetic Lethality Homo sapiens
78 CCDC18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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