Gene description for ARFGEF2
Gene name ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Gene symbol ARFGEF2
Other names/aliases BIG2
PVNH2
dJ1164I10.1
Species Homo sapiens
 Database cross references - ARFGEF2
ExoCarta ExoCarta_10564
Entrez Gene 10564
HGNC 15853
MIM 605371
UniProt Q9Y6D5  
 ARFGEF2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Endothelial cells 26027894    
 Gene ontology annotations for ARFGEF2
Molecular Function
    protein binding GO:0005515 IPI
    GABA receptor binding GO:0050811 ISS
    ARF guanyl-nucleotide exchange factor activity GO:0005086 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    protein kinase A regulatory subunit binding GO:0034237 IDA
    myosin binding GO:0017022 IEA
Biological Process
    protein transport GO:0015031 IEA
    receptor recycling GO:0001881 IDA
    intracellular signal transduction GO:0035556 IDA
    endomembrane system organization GO:0010256 IMP
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    exocytosis GO:0006887 IEA
    Golgi to plasma membrane transport GO:0006893 IMP
    endosome organization GO:0007032 IMP
    positive regulation of GTPase activity GO:0043547 IEA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IEA
    cytoplasmic membrane-bounded vesicle GO:0016023 IEA
    Golgi membrane GO:0000139 IDA
    cytoplasmic vesicle GO:0031410 ISS
    cytosol GO:0005829 IDA
    membrane GO:0016020 IDA
    symmetric synapse GO:0032280 ISS
    trans-Golgi network GO:0005802 ISS
    axonemal microtubule GO:0005879 ISS
    asymmetric synapse GO:0032279 ISS
    cell junction GO:0030054 IEA
    dendritic spine GO:0043197 ISS
    microtubule organizing center GO:0005815 IDA
    recycling endosome GO:0055037 IDA
 Experiment description of studies that identified ARFGEF2 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for ARFGEF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARFGEF1  
Invivo Homo sapiens
2 PRKAR1A 5573
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
3 FKBP2  
Affinity Capture-Western Homo sapiens
4 PRKAR2A 5576
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which ARFGEF2 is involved
PathwayEvidenceSource
Association of TriC/CCT with target proteins during biosynthesis TAS Reactome





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