Gene description for MLST8
Gene name MTOR associated protein, LST8 homolog (S. cerevisiae)
Gene symbol MLST8
Other names/aliases GBL
GbetaL
LST8
POP3
WAT1
Species Homo sapiens
 Database cross references - MLST8
ExoCarta ExoCarta_64223
Vesiclepedia VP_64223
Entrez Gene 64223
HGNC 24825
MIM 612190
UniProt Q9BVC4  
 MLST8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for MLST8
Molecular Function
    protein binding GO:0005515 IPI
    protein-macromolecule adaptor activity GO:0030674 IDA
    protein serine/threonine kinase activator activity GO:0043539 IDA
Biological Process
    DNA damage response GO:0006974 NAS
    cytoskeleton organization GO:0007010 NAS
    negative regulation of autophagy GO:0010507 NAS
    positive regulation of cell growth GO:0030307 NAS
    positive regulation of actin filament polymerization GO:0030838 IEA
    cellular response to nutrient levels GO:0031669 NAS
    TOR signaling GO:0031929 IBA
    positive regulation of TOR signaling GO:0032008 IMP
    regulation of actin cytoskeleton organization GO:0032956 IBA
    regulation of actin cytoskeleton organization GO:0032956 IMP
    TORC1 signaling GO:0038202 IDA
    TORC1 signaling GO:0038202 IMP
    TORC2 signaling GO:0038203 IDA
    negative regulation of apoptotic process GO:0043066 NAS
    positive regulation of glycolytic process GO:0045821 NAS
    positive regulation of lipid biosynthetic process GO:0046889 NAS
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IEA
    cellular response to hypoxia GO:0071456 NAS
    cellular response to osmotic stress GO:0071470 NAS
    positive regulation of pentose-phosphate shunt GO:1905857 NAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 ISS
    lysosomal membrane GO:0005765 IEA
    cytosol GO:0005829 TAS
    TORC1 complex GO:0031931 IBA
    TORC1 complex GO:0031931 IDA
    TORC1 complex GO:0031931 IPI
    TORC2 complex GO:0031932 IBA
    TORC2 complex GO:0031932 IDA
    TORC2 complex GO:0031932 IPI
 Experiment description of studies that identified MLST8 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MLST8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCT3 7203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HSPA6 3310
Affinity Capture-MS Homo sapiens
3 HELZ 9931
Affinity Capture-MS Homo sapiens
4 FOXF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 TCF7L2  
Affinity Capture-MS Homo sapiens
6 FPR1  
Affinity Capture-MS Homo sapiens
7 SETX  
Affinity Capture-MS Homo sapiens
8 CCT4 10575
Affinity Capture-MS Homo sapiens
9 CCT6B 10693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PRR5  
Affinity Capture-Western Homo sapiens
11 SLC30A4 7782
Affinity Capture-MS Homo sapiens
12 Cct2 12461
Affinity Capture-MS Mus musculus
13 APEX1 328
Affinity Capture-RNA Homo sapiens
14 RUVBL1 8607
Affinity Capture-MS Homo sapiens
15 HSPA2 3306
Affinity Capture-MS Homo sapiens
16 Mtor  
Affinity Capture-Western Mus musculus
17 COPB2 9276
Affinity Capture-MS Homo sapiens
18 ANKRD49  
Affinity Capture-MS Homo sapiens
19 FBXW7  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 KRT85  
Affinity Capture-MS Homo sapiens
21 OTUD7B 56957
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 Cct4 12464
Affinity Capture-MS Mus musculus
23 WDR5 11091
Affinity Capture-Western Homo sapiens
24 HSPA8 3312
Affinity Capture-MS Homo sapiens
25 MELK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 KIAA1715 80856
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
28 BAG5 9529
Affinity Capture-MS Homo sapiens
29 Cct3 12462
Affinity Capture-MS Mus musculus
30 ACAT2 39
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TCP1 6950
Affinity Capture-MS Homo sapiens
32 Cul4a  
Affinity Capture-Western Mus musculus
33 A2M 2
Two-hybrid Homo sapiens
34 TUBA1A 7846
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 ILVBL 10994
Affinity Capture-MS Homo sapiens
36 CCT5 22948
Affinity Capture-MS Homo sapiens
37 Srp72  
Affinity Capture-MS Mus musculus
38 NUMB 8650
Affinity Capture-Western Homo sapiens
39 CCT8 10694
Affinity Capture-MS Homo sapiens
40 GPR182  
Affinity Capture-MS Homo sapiens
41 TRAPPC2L 51693
Affinity Capture-MS Homo sapiens
42 LYPLA2 11313
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 KIF11 3832
Affinity Capture-MS Homo sapiens
44 ULK1  
Affinity Capture-Western Homo sapiens
45 EIF4EBP1  
Biochemical Activity Homo sapiens
46 ZNF609  
Affinity Capture-MS Homo sapiens
47 STUB1 10273
Affinity Capture-MS Homo sapiens
48 BAG3 9531
Affinity Capture-MS Homo sapiens
49 ATXN1 6310
Two-hybrid Homo sapiens
50 CD40 958
Affinity Capture-MS Homo sapiens
51 CYB5B 80777
Affinity Capture-MS Homo sapiens
52 XPO1 7514
Affinity Capture-MS Homo sapiens
53 EGLN3  
Affinity Capture-MS Homo sapiens
54 CCT6A 908
Affinity Capture-MS Homo sapiens
55 NPTN 27020
Affinity Capture-MS Homo sapiens
56 RICTOR 253260
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
57 USP54 159195
Affinity Capture-MS Homo sapiens
58 EGFR 1956
Negative Genetic Homo sapiens
59 FKBP8 23770
Affinity Capture-Western Homo sapiens
60 SUZ12  
Affinity Capture-MS Homo sapiens
61 NXF1 10482
Affinity Capture-RNA Homo sapiens
62 XPO6 23214
Affinity Capture-MS Homo sapiens
63 TUBA4A 7277
Affinity Capture-MS Homo sapiens
64 CDK2 1017
Biochemical Activity Homo sapiens
65 SERP2  
Affinity Capture-MS Homo sapiens
66 XPO4 64328
Affinity Capture-MS Homo sapiens
67 WDR60 55112
Affinity Capture-MS Homo sapiens
68 SAV1  
Affinity Capture-MS Homo sapiens
69 MTOR 2475
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
70 MAPKAP1 79109
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
71 MPZL1 9019
Affinity Capture-MS Homo sapiens
72 ETV3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 Cct8 12469
Affinity Capture-MS Mus musculus
74 SCN2B  
Affinity Capture-MS Homo sapiens
75 PDCL3 79031
Affinity Capture-MS Homo sapiens
76 THADA 63892
Affinity Capture-MS Homo sapiens
77 Rptor  
Affinity Capture-Western Mus musculus
78 PFDN5 5204
Affinity Capture-MS Homo sapiens
79 XXYLT1 152002
Affinity Capture-MS Homo sapiens
80 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
81 FASN 2194
Positive Genetic Homo sapiens
82 DEPTOR 64798
Affinity Capture-MS Homo sapiens
83 CCDC167  
Affinity Capture-MS Homo sapiens
84 VOPP1  
Affinity Capture-MS Homo sapiens
85 MTMR3  
Affinity Capture-Western Homo sapiens
86 Dtl  
Affinity Capture-Western Mus musculus
87 TRMT2A 27037
Affinity Capture-MS Homo sapiens
88 KEAP1 9817
Affinity Capture-MS Homo sapiens
89 RPTOR 57521
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
90 SQSTM1 8878
Affinity Capture-Western Homo sapiens
91 CDK1 983
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
92 CCT7 10574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 PRAME  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 ZNRF2 223082
Affinity Capture-Western Homo sapiens
95 MOV10 4343
Affinity Capture-RNA Homo sapiens
96 KDELR2 11014
Affinity Capture-MS Homo sapiens
97 ZUFSP 221302
Affinity Capture-MS Homo sapiens
98 CLMP 79827
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 PRMT1 3276
Two-hybrid Homo sapiens
100 HSPA1B 3304
Affinity Capture-MS Homo sapiens
101 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MLST8 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Amino acids regulate mTORC1 TAS Reactome
Autophagy TAS Reactome
CD28 co-stimulation TAS Reactome
CD28 dependent PI3K/Akt signaling TAS Reactome
Cellular response to heat stress TAS Reactome
Cellular response to starvation TAS Reactome
Cellular responses to mechanical stimuli TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Constitutive Signaling by AKT1 E17K in Cancer TAS Reactome
Costimulation by the CD28 family TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Energy dependent regulation of mTOR by LKB1-AMPK TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells TAS Reactome
HSF1-dependent transactivation TAS Reactome
Immune System TAS Reactome
Intracellular signaling by second messengers TAS Reactome
Macroautophagy TAS Reactome
MTOR signalling TAS Reactome
mTORC1-mediated signalling TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PIP3 activates AKT signaling TAS Reactome
PTEN Regulation TAS Reactome
Regulation of PTEN gene transcription TAS Reactome
Regulation of TP53 Activity TAS Reactome
Regulation of TP53 Degradation TAS Reactome
Regulation of TP53 Expression and Degradation TAS Reactome
Response of endothelial cells to shear stress TAS Reactome
RNA Polymerase II Transcription TAS Reactome
Signal Transduction TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by VEGF TAS Reactome
TP53 Regulates Metabolic Genes TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome
VEGFR2 mediated vascular permeability TAS Reactome





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