Gene description for SYNJ2
Gene name synaptojanin 2
Gene symbol SYNJ2
Other names/aliases INPP5H
Species Homo sapiens
 Database cross references - SYNJ2
ExoCarta ExoCarta_8871
Vesiclepedia VP_8871
Entrez Gene 8871
HGNC 11504
MIM 609410
UniProt O15056  
 SYNJ2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
 Gene ontology annotations for SYNJ2
Molecular Function
    RNA binding GO:0003723 IEA
    phosphatidylinositol-3-phosphate phosphatase activity GO:0004438 TAS
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 IBA
    phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity GO:0004439 TAS
    protein binding GO:0005515 IPI
    phosphatidylinositol phosphate 4-phosphatase activity GO:0034596 TAS
    phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity GO:0043813 TAS
    phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity GO:0052629 TAS
    inositol-1,4,5-trisphosphate 5-phosphatase activity GO:0052658 IBA
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IEA
    phosphatidylinositol dephosphorylation GO:0046856 IEA
    synaptic vesicle endocytosis GO:0048488 IBA
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IMP
    synaptic vesicle endocytosis GO:0048488 NAS
    membrane organization GO:0061024 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 IBA
    cell projection GO:0042995 IEA
    membrane raft GO:0045121 IEA
    perinuclear region of cytoplasm GO:0048471 IBA
    presynapse GO:0098793 IBA
 Experiment description of studies that identified SYNJ2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for SYNJ2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 SFN 2810
Affinity Capture-MS Homo sapiens
3 RAC1 5879
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 ITSN1 6453
Two-hybrid Homo sapiens
5 GRB2 2885
Two-hybrid Homo sapiens
6 Cd2ap 12488
Affinity Capture-MS Mus musculus
7 FBXL6  
Proximity Label-MS Homo sapiens
8 YWHAB 7529
Affinity Capture-MS Homo sapiens
9 PFN1 5216
Proximity Label-MS Homo sapiens
10 YWHAE 7531
Affinity Capture-MS Homo sapiens
11 MIB1 57534
Proximity Label-MS Homo sapiens
12 SQSTM1 8878
Proximity Label-MS Homo sapiens
13 DDX58 23586
Affinity Capture-RNA Homo sapiens
14 MAPRE1 22919
Proximity Label-MS Homo sapiens
15 ANAPC2 29882
Proximity Label-MS Homo sapiens
16 MAPRE3  
Proximity Label-MS Homo sapiens
17 ITSN2 50618
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
18 CDH1 999
Proximity Label-MS Homo sapiens
19 RPA3 6119
Proximity Label-MS Homo sapiens
20 SYNJ2BP 55333
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
21 CLTA 1211
Proximity Label-MS Homo sapiens
22 DCTN1 1639
Proximity Label-MS Homo sapiens
23 HIST2H3A 333932
Cross-Linking-MS (XL-MS) Homo sapiens
24 BICD2 23299
Proximity Label-MS Homo sapiens
25 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
26 GOLM1 51280
Cross-Linking-MS (XL-MS) Homo sapiens
27 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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