Gene description for DNM1
Gene name dynamin 1
Gene symbol DNM1
Other names/aliases DNM
EIEE31
Species Homo sapiens
 Database cross references - DNM1
ExoCarta ExoCarta_1759
Entrez Gene 1759
HGNC 2972
MIM 602377
UniProt Q05193  
 DNM1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for DNM1
Molecular Function
    GTP binding GO:0005525 IEA
    protein binding GO:0005515 IPI
    nitric-oxide synthase binding GO:0050998 IEA
    identical protein binding GO:0042802 IPI
    protein complex binding GO:0032403 IEA
    protein kinase binding GO:0019901 ISS
    GTPase activity GO:0003924 IEA
    poly(A) RNA binding GO:0044822 IDA
    protein C-terminus binding GO:0008022 IEA
Biological Process
    endosome organization GO:0007032 IMP
    receptor-mediated endocytosis GO:0006898 IMP
    receptor internalization GO:0031623 IEA
    clathrin-mediated endocytosis GO:0072583 IEA
    ephrin receptor signaling pathway GO:0048013 TAS
    endocytosis GO:0006897 TAS
    axon guidance GO:0007411 TAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    extracellular exosome GO:0070062 IDA
    microtubule GO:0005874 IEA
    membrane coat GO:0030117 IEA
 Experiment description of studies that identified DNM1 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DNM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PFN2 5217
Invivo Homo sapiens
Invitro Homo sapiens
2 DNM1 1759
Invitro Homo sapiens
Invitro Homo sapiens
3 NCK1 4690
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
4 CLINT1 9685
Reconstituted Complex Homo sapiens
5 PACSIN1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 SH3GL1 6455
Reconstituted Complex Homo sapiens
7 FER  
Co-fractionation Homo sapiens
8 CTTN 2017
Invitro Homo sapiens
Invivo Homo sapiens
9 PACSIN2 11252
Reconstituted Complex Homo sapiens
10 ASB13  
Two-hybrid Homo sapiens
11 CABIN1  
Invivo Homo sapiens
12 Eps15  
Reconstituted Complex Mus musculus
13 PACSIN3 29763
Reconstituted Complex Homo sapiens
14 KIAA1377  
Two-hybrid Homo sapiens
15 DNMBP  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 SH3GLB1 51100
Reconstituted Complex Homo sapiens
17 FNBP1 23048
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 PIN4  
Two-hybrid Homo sapiens
19 MED31  
Two-hybrid Homo sapiens
20 AMPH  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
21 MAP3K10  
Reconstituted Complex Homo sapiens
22 SH3GL2  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
23 BIN1 274
Invitro Homo sapiens
Invitro Homo sapiens
24 GRB2 2885
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml



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