Gene description for ZAK
Gene name sterile alpha motif and leucine zipper containing kinase AZK
Gene symbol ZAK
Other names/aliases AZK
MLK7
MLT
MLTK
MRK
mlklak
pk
Species Homo sapiens
 Database cross references - ZAK
ExoCarta ExoCarta_51776
Vesiclepedia VP_51776
Entrez Gene 51776
MIM 609479
UniProt Q9NYL2  
 ZAK identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ZAK
Molecular Function
    magnesium ion binding GO:0000287 IDA
    RNA binding GO:0003723 HDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    JUN kinase kinase kinase activity GO:0004706 IDA
    MAP kinase kinase kinase activity GO:0004709 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    protein kinase regulator activity GO:0019887 IDA
    protein kinase activator activity GO:0030295 IDA
    ribosome binding GO:0043022 IDA
    small ribosomal subunit rRNA binding GO:0070181 IDA
    protein serine kinase activity GO:0106310 IEA
    stalled ribosome sensor activity GO:0170011 IDA
Biological Process
    DNA damage checkpoint signaling GO:0000077 IMP
    MAPK cascade GO:0000165 IDA
    MAPK cascade GO:0000165 IEA
    protein phosphorylation GO:0006468 IDA
    inflammatory response GO:0006954 IMP
    cytoskeleton organization GO:0007010 IEA
    chromosome segregation GO:0007059 IBA
    JNK cascade GO:0007254 IDA
    cell death GO:0008219 NAS
    regulation of mitotic metaphase/anaphase transition GO:0030071 IBA
    cell differentiation GO:0030154 NAS
    stress-activated protein kinase signaling cascade GO:0031098 IDA
    p38MAPK cascade GO:0038066 IDA
    embryonic digit morphogenesis GO:0042733 IMP
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of programmed cell death GO:0043068 IDA
    protein autophosphorylation GO:0046777 IDA
    stress-activated MAPK cascade GO:0051403 IDA
    limb development GO:0060173 IMP
    pyroptotic inflammatory response GO:0070269 IDA
    negative regulation of stress-activated protein kinase signaling cascade GO:0070303 IDA
    cellular response to gamma radiation GO:0071480 IDA
    cellular response to UV-B GO:0071493 IDA
    GCN2-mediated signaling GO:0140469 IDA
    negative regulation of translation in response to endoplasmic reticulum stress GO:1902010 IDA
    positive regulation of mitotic DNA damage checkpoint GO:1904291 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified ZAK in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ZAK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 YWHAH 7533
Affinity Capture-MS Homo sapiens
3 MPP1 4354
Two-hybrid Homo sapiens
4 SFN 2810
Affinity Capture-MS Homo sapiens
5 RPS6KA5 9252
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
6 BCL11A 53335
Affinity Capture-MS Homo sapiens
7 ZWINT  
Affinity Capture-MS Homo sapiens
8 EMC2 9694
Affinity Capture-MS Homo sapiens
9 PSPC1 55269
Affinity Capture-MS Homo sapiens
10 PBX2  
Affinity Capture-MS Homo sapiens
11 MAP2K7 5609
Affinity Capture-Western Homo sapiens
12 ZAK 51776
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 ITSN1 6453
Two-hybrid Homo sapiens
14 MIDN 90007
Two-hybrid Homo sapiens
15 RNF19A  
Two-hybrid Homo sapiens
16 BCL11B  
Affinity Capture-MS Homo sapiens
17 MEIS1  
Affinity Capture-MS Homo sapiens
18 TP53 7157
Affinity Capture-MS Homo sapiens
19 ZNF593  
Two-hybrid Homo sapiens
20 TCEAL7  
Affinity Capture-MS Homo sapiens
21 RAB5A 5868
Affinity Capture-MS Homo sapiens
22 ZNF296  
Affinity Capture-MS Homo sapiens
23 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SERF2 10169
Affinity Capture-MS Homo sapiens
25 ZNF746  
Two-hybrid Homo sapiens
26 XPO1 7514
Affinity Capture-MS Homo sapiens
27 AURKA 6790
Affinity Capture-MS Homo sapiens
28 ANAPC15  
Affinity Capture-MS Homo sapiens
29 VPS33B 26276
Two-hybrid Homo sapiens
30 CDC37 11140
Affinity Capture-MS Homo sapiens
31 Nedd4 25489
Biochemical Activity Rattus norvegicus
32 PBX1 5087
Affinity Capture-MS Homo sapiens
33 ZFP1  
Affinity Capture-MS Homo sapiens
34 RPL10 6134
Affinity Capture-MS Homo sapiens
35 YWHAZ 7534
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ZNF71  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 RNF138  
Affinity Capture-MS Homo sapiens
38 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
39 INPP4B 8821
Co-fractionation Homo sapiens
40 RBX1 9978
Two-hybrid Homo sapiens
41 EZH1  
Affinity Capture-MS Homo sapiens
42 MKI67  
Affinity Capture-MS Homo sapiens
43 ZFP2  
Two-hybrid Homo sapiens
44 Cdk1 12534
Affinity Capture-MS Mus musculus
45 CDC42 998
Affinity Capture-MS Homo sapiens
46 TGFBR1 7046
Affinity Capture-Western Homo sapiens
47 C20orf197  
Affinity Capture-MS Homo sapiens
48 MEIS2  
Affinity Capture-MS Homo sapiens
49 XRCC1 7515
Affinity Capture-MS Homo sapiens
50 MAP2K3 5606
Co-fractionation Homo sapiens
51 RPS20 6224
Proximity Label-MS Homo sapiens
52 EZH2  
Two-hybrid Homo sapiens
53 MYC  
Affinity Capture-MS Homo sapiens
54 YWHAE 7531
Affinity Capture-MS Homo sapiens
55 ZNF544  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 ZNF775  
Two-hybrid Homo sapiens
57 Mis12  
Affinity Capture-MS Mus musculus
58 ZNF567  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
59 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
60 GAGE5  
Affinity Capture-MS Homo sapiens
61 HSPA1A 3303
Affinity Capture-MS Homo sapiens
62 SMARCB1 6598
Two-hybrid Homo sapiens
63 SERBP1 26135
Proximity Label-MS Homo sapiens
64 YWHAQ 10971
Affinity Capture-MS Homo sapiens
65 COL8A1 1295
Affinity Capture-MS Homo sapiens
66 CCDC69 26112
Two-hybrid Homo sapiens
67 UNC119 9094
Two-hybrid Homo sapiens
68 ZNF195  
Affinity Capture-MS Homo sapiens
69 YWHAG 7532
Affinity Capture-MS Homo sapiens
70 SPCS1 28972
Affinity Capture-MS Homo sapiens
71 CRMP1 1400
Two-hybrid Homo sapiens
72 PARD6A  
Affinity Capture-MS Homo sapiens
73 PSENEN  
Affinity Capture-MS Homo sapiens
74 PBX3  
Affinity Capture-MS Homo sapiens
75 DDAH2 23564
Two-hybrid Homo sapiens
76 NEDD4L 23327
Biochemical Activity Homo sapiens
77 ZNF33A 7581
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
78 IGHM 3507
Two-hybrid Homo sapiens
79 ZNF35  
Affinity Capture-MS Homo sapiens
80 ZNF227  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ZAK is involved
No pathways found





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