Gene description for CDH2
Gene name cadherin 2, type 1, N-cadherin (neuronal)
Gene symbol CDH2
Other names/aliases CD325
CDHN
CDw325
NCAD
Species Homo sapiens
 Database cross references - CDH2
ExoCarta ExoCarta_1000
Entrez Gene 1000
HGNC 1759
MIM 114020
UniProt P19022  
 CDH2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Melanoma cells 25950383    
Melanoma cells 25950383    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CDH2
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein phosphatase binding GO:0019903 IEA
    protein binding GO:0005515 IPI
    alpha-catenin binding GO:0045294 IPI
    gamma-catenin binding GO:0045295 IPI
    beta-catenin binding GO:0008013 TAS
Biological Process
    cell migration GO:0016477 IEA
    adherens junction organization GO:0034332 TAS
    cell-cell adhesion mediated by cadherin GO:0044331 ISS
    calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules GO:0016339 IEA
    positive regulation of MAPK cascade GO:0043410 IEA
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IEA
    cell-cell junction organization GO:0045216 TAS
    cell junction assembly GO:0034329 TAS
    cell adhesion GO:0007155 TAS
    neuronal stem cell maintenance GO:0097150 ISS
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 IEA
    striated muscle cell differentiation GO:0051146 IEA
    muscle cell differentiation GO:0042692 TAS
    glial cell differentiation GO:0010001 ISS
    blood vessel morphogenesis GO:0048514 IEA
    positive regulation of muscle cell differentiation GO:0051149 TAS
    negative regulation of canonical Wnt signaling pathway GO:0090090 IEA
Subcellular Localization
    intercalated disc GO:0014704 ISS
    integral component of membrane GO:0016021 IEA
    cell-cell adherens junction GO:0005913 IDA
    fascia adherens GO:0005916 IEA
    catenin complex GO:0016342 IDA
    focal adhesion GO:0005925 IDA
    lamellipodium GO:0030027 IDA
    synapse GO:0045202 IEA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    cortical actin cytoskeleton GO:0030864 IDA
    extracellular exosome GO:0070062 IDA
    apical plasma membrane GO:0016324 IEA
 Experiment description of studies that identified CDH2 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDH2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB8B 51762
Affinity Capture-Western Homo sapiens
2 CTNNB1 1499
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
3 GNA13 10672
Reconstituted Complex Homo sapiens
4 CDON  
Affinity Capture-Western Homo sapiens
5 CTNNA1 1495
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 ACTA1 58
Affinity Capture-Western Homo sapiens
7 GNA12 2768
Reconstituted Complex Homo sapiens
8 CDH4  
Invivo Homo sapiens
9 LRRC7  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 JUP 3728
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
11 BOC  
Affinity Capture-Western Homo sapiens
12 PKP4 8502
Two-hybrid Homo sapiens
13 PTPN1 5770
Invitro Homo sapiens
14 CDH11 1009
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CTNND1 1500
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which CDH2 is involved
PathwayEvidenceSource
Adherens junctions interactions TAS Reactome
CDO in myogenesis TAS Reactome





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