Gene description for SMG8
Gene name SMG8 nonsense mediated mRNA decay factor
Gene symbol SMG8
Other names/aliases C17orf71
Species Homo sapiens
 Database cross references - SMG8
ExoCarta ExoCarta_55181
Vesiclepedia VP_55181
Entrez Gene 55181
HGNC 25551
MIM 613175
UniProt Q8ND04  
 SMG8 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SMG8
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IBA
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IMP
    regulation of protein kinase activity GO:0045859 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SMG8 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SMG8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 LRRC48  
Affinity Capture-MS Homo sapiens
3 ANLN 54443
Affinity Capture-MS Homo sapiens
4 GALNT10 55568
Affinity Capture-MS Homo sapiens
5 IL17RA 23765
Affinity Capture-MS Homo sapiens
6 IARS2 55699
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TPGS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 NAAA  
Affinity Capture-MS Homo sapiens
9 LRRC49  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SIRT6  
Affinity Capture-MS Homo sapiens
11 NPRL2  
Affinity Capture-MS Homo sapiens
12 D2HGDH 728294
Affinity Capture-MS Homo sapiens
13 AGBL4  
Affinity Capture-MS Homo sapiens
14 TSSC1 7260
Affinity Capture-MS Homo sapiens
15 CETN3  
Affinity Capture-MS Homo sapiens
16 RBM8A 9939
Affinity Capture-Western Homo sapiens
17 ANAPC4 29945
Affinity Capture-MS Homo sapiens
18 DOCK5 80005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CIB3  
Affinity Capture-MS Homo sapiens
20 BAG2 9532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 HCST  
Affinity Capture-MS Homo sapiens
22 EFNA1  
Affinity Capture-MS Homo sapiens
23 NCAPH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 BBS7 55212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CTAG1B  
Affinity Capture-MS Homo sapiens
26 P4HA2 8974
Affinity Capture-MS Homo sapiens
27 CRYL1 51084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MFI2 4241
Affinity Capture-MS Homo sapiens
29 NPTN 27020
Affinity Capture-MS Homo sapiens
30 SULT1C4  
Affinity Capture-MS Homo sapiens
31 AZIN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SPC24 147841
Affinity Capture-MS Homo sapiens
33 LRRC74A  
Affinity Capture-MS Homo sapiens
34 FKBP5 2289
Affinity Capture-MS Homo sapiens
35 PDDC1 347862
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 TKT 7086
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 DLK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 METTL21B  
Affinity Capture-MS Homo sapiens
39 B4GALT2  
Affinity Capture-MS Homo sapiens
40 MAGEA9  
Affinity Capture-MS Homo sapiens
41 DUSP16  
Affinity Capture-MS Homo sapiens
42 NPAS1  
Affinity Capture-MS Homo sapiens
43 HDHD1  
Affinity Capture-MS Homo sapiens
44 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
45 PRPS2 5634
Affinity Capture-MS Homo sapiens
46 SF3B3 23450
Affinity Capture-MS Homo sapiens
47 CRYBB3  
Affinity Capture-MS Homo sapiens
48 PPP4R1L  
Affinity Capture-MS Homo sapiens
49 SCGB2A2  
Affinity Capture-MS Homo sapiens
50 C12orf74  
Affinity Capture-MS Homo sapiens
51 TSC22D4 81628
Affinity Capture-MS Homo sapiens
52 SMG9 56006
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
53 DNAI2  
Affinity Capture-MS Homo sapiens
54 TUBB 203068
Affinity Capture-MS Homo sapiens
55 NT5C 30833
Affinity Capture-MS Homo sapiens
56 NTRK1 4914
Affinity Capture-MS Homo sapiens
57 UPF1 5976
Affinity Capture-Western Homo sapiens
58 C1orf54  
Affinity Capture-MS Homo sapiens
59 CDC16 8881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 RFXANK  
Affinity Capture-MS Homo sapiens
61 PPARD  
Affinity Capture-MS Homo sapiens
62 SPHK1 8877
Affinity Capture-MS Homo sapiens
63 SMG1 23049
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
64 THBS3 7059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 LDLR 3949
Positive Genetic Homo sapiens
66 PTGES3 10728
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 ACTL6B  
Affinity Capture-MS Homo sapiens
68 FAM167A  
Affinity Capture-MS Homo sapiens
69 F9 2158
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 NICN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 GUSBP5  
Affinity Capture-MS Homo sapiens
72 SLC31A1 1317
Affinity Capture-MS Homo sapiens
73 COMMD5 28991
Affinity Capture-MS Homo sapiens
74 PCDHB3  
Affinity Capture-MS Homo sapiens
75 SDF4 51150
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 INF2 64423
Affinity Capture-MS Homo sapiens
77 LYPD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 ATG7 10533
Affinity Capture-MS Homo sapiens
79 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 HSP90AA4P 3323
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 UBXN6 80700
Affinity Capture-MS Homo sapiens
82 FAM160B2 64760
Affinity Capture-MS Homo sapiens
83 CAPN2 824
Affinity Capture-MS Homo sapiens
84 KLHL14 57565
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here