Gene description for ECM1
Gene name extracellular matrix protein 1
Gene symbol ECM1
Other names/aliases URBWD
Species Homo sapiens
 Database cross references - ECM1
ExoCarta ExoCarta_1893
Vesiclepedia VP_1893
Entrez Gene 1893
HGNC 3153
MIM 602201
UniProt Q16610  
 ECM1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Keratinocytes 19530224    
Keratinocytes 19530224    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Saliva 19199708    
 Gene ontology annotations for ECM1
Molecular Function
    protease binding GO:0002020 IPI
    interleukin-2 receptor binding GO:0005134 IEA
    extracellular matrix structural constituent GO:0005201 RCA
    protein binding GO:0005515 IPI
Biological Process
    ossification GO:0001503 IEA
    angiogenesis GO:0001525 IEA
    positive regulation of endothelial cell proliferation GO:0001938 IDA
    negative regulation of cytokine-mediated signaling pathway GO:0001960 IEA
    chondrocyte development GO:0002063 IEA
    regulation of type 2 immune response GO:0002828 IEA
    endochondral bone growth GO:0003416 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    inflammatory response GO:0006954 IEA
    signal transduction GO:0007165 IEA
    negative regulation of peptidase activity GO:0010466 IDA
    regulation of bone mineralization GO:0030500 IBA
    negative regulation of bone mineralization GO:0030502 IDA
    biomineral tissue development GO:0031214 IEA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 HMP
    positive regulation of angiogenesis GO:0045766 IDA
    regulation of T cell migration GO:2000404 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular matrix GO:0031012 NAS
    platelet dense granule lumen GO:0031089 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
    collagen-containing extracellular matrix GO:0062023 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ECM1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
13
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
14
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for ECM1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HMP19  
Affinity Capture-MS Homo sapiens
2 COL6A1 1291
Affinity Capture-MS Homo sapiens
3 SAMD11  
Two-hybrid Homo sapiens
4 ENKD1  
Two-hybrid Homo sapiens
5 LCE2A  
Two-hybrid Homo sapiens
6 FRS3 10817
Two-hybrid Homo sapiens
7 MKRN3  
Two-hybrid Homo sapiens
8 BNIP3L  
Two-hybrid Homo sapiens
9 ZIC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ATN1  
Two-hybrid Homo sapiens
11 GNE 10020
Two-hybrid Homo sapiens
12 SOX2  
Affinity Capture-MS Homo sapiens
13 PI4KA 5297
Affinity Capture-MS Homo sapiens
14 COX20  
Affinity Capture-MS Homo sapiens
15 CTU2 348180
Affinity Capture-MS Homo sapiens
16 DPPA4  
Affinity Capture-MS Homo sapiens
17 ZDHHC19  
Affinity Capture-MS Homo sapiens
18 CA8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 IL2RB  
Affinity Capture-Western Homo sapiens
20 E2F6 1876
Affinity Capture-MS Homo sapiens
21 SNRPC 6631
Two-hybrid Homo sapiens
22 UBQLN1 29979
Two-hybrid Homo sapiens
23 MRPL39 54148
Affinity Capture-MS Homo sapiens
24 CUL1 8454
Affinity Capture-MS Homo sapiens
25 AHDC1  
Affinity Capture-MS Homo sapiens
26 YAF2  
Affinity Capture-MS Homo sapiens
27 LMO4  
Two-hybrid Homo sapiens
28 MAPK1 5594
Affinity Capture-MS Homo sapiens
29 POLD1 5424
Affinity Capture-MS Homo sapiens
30 FASLG 356
Two-hybrid Homo sapiens
31 MAPK3 5595
Affinity Capture-MS Homo sapiens
32 TGM2 7052
Affinity Capture-MS Homo sapiens
33 SLC9A5  
Affinity Capture-MS Homo sapiens
34 STX17 55014
Affinity Capture-MS Homo sapiens
35 SRRT 51593
Affinity Capture-MS Homo sapiens
36 CCDC51 79714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CYSRT1 375791
Two-hybrid Homo sapiens
38 SARAF  
Affinity Capture-MS Homo sapiens
39 ALDH3A1 218
Affinity Capture-MS Homo sapiens
40 DDX39B 7919
Affinity Capture-MS Homo sapiens
41 CYLD  
Affinity Capture-MS Homo sapiens
42 SRPK1 6732
Biochemical Activity Homo sapiens
43 C22orf39  
Affinity Capture-MS Homo sapiens
44 RNASEH1 246243
Affinity Capture-MS Homo sapiens
45 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
46 CCNC  
Affinity Capture-MS Homo sapiens
47 RERE 473
Two-hybrid Homo sapiens
48 TBC1D22B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 UBQLN2 29978
Two-hybrid Homo sapiens
50 KLHL38  
Two-hybrid Homo sapiens
51 SLC35F2 54733
Affinity Capture-MS Homo sapiens
52 CD274 29126
Affinity Capture-MS Homo sapiens
53 KRTAP9-3  
Two-hybrid Homo sapiens
54 ZSCAN12  
Affinity Capture-MS Homo sapiens
55 NPPA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 XRCC6BP1  
Affinity Capture-MS Homo sapiens
57 RBFOX1 54715
Affinity Capture-MS Homo sapiens
58 TRIM68 55128
Affinity Capture-MS Homo sapiens
59 ACTN3 89
Two-hybrid Homo sapiens
60 FTH1 2495
Affinity Capture-MS Homo sapiens
61 PI4K2A 55361
Affinity Capture-MS Homo sapiens
62 SNTG1  
Affinity Capture-MS Homo sapiens
63 VHL  
Affinity Capture-MS Homo sapiens
64 CFI 3426
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 FN1 2335
Affinity Capture-MS Homo sapiens
66 C21orf59 56683
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 LIN7A 8825
Two-hybrid Homo sapiens
68 SGTB  
Two-hybrid Homo sapiens
69 GPANK1  
Two-hybrid Homo sapiens
70 FRMD1  
Affinity Capture-MS Homo sapiens
71 PI4KAP1  
Affinity Capture-MS Homo sapiens
72 HECW2  
Affinity Capture-MS Homo sapiens
73 A1CF  
Two-hybrid Homo sapiens
74 HIBADH 11112
Affinity Capture-MS Homo sapiens
75 ITGB4 3691
Two-hybrid Homo sapiens
76 PLCZ1  
Affinity Capture-MS Homo sapiens
77 GCM2  
Two-hybrid Homo sapiens
78 CTU1 90353
Affinity Capture-MS Homo sapiens
79 GMCL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 MMRN1 22915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 IRAK3 11213
Two-hybrid Homo sapiens
82 DDIAS  
Two-hybrid Homo sapiens
83 BCAS2 10286
Two-hybrid Homo sapiens
84 AMTN  
Affinity Capture-MS Homo sapiens
85 SH2D3A  
Affinity Capture-MS Homo sapiens
86 MCL1 4170
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 NHLRC2 374354
Affinity Capture-MS Homo sapiens
88 RNPC3  
Affinity Capture-MS Homo sapiens
89 ERCC3  
Affinity Capture-MS Homo sapiens
90 TEFM  
Affinity Capture-MS Homo sapiens
91 TRIM31  
Affinity Capture-MS Homo sapiens
92 C1orf109  
Two-hybrid Homo sapiens
93 GLYR1 84656
Affinity Capture-MS Homo sapiens
94 KRTAP5-3  
Two-hybrid Homo sapiens
95 CPTP  
Two-hybrid Homo sapiens
View the network image/svg+xml



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