ExoCarta: Gene summary

Credits | Contact

Gene description for FLG
Gene name	filaggrin
Gene symbol	FLG
Other names/aliases	ATOD2
Species	Homo sapiens
Database cross references - FLG
ExoCarta	ExoCarta_2312
Vesiclepedia	VP_2312
Entrez Gene	2312
HGNC	3748
MIM	135940
UniProt	P20930
FLG identified in exosomes derived from the following tissue/cell type
Chondrocytes	35931686
Chondrocytes	35931686

Gene ontology annotations for FLG

Molecular Function
	calcium ion binding	GO:0005509	IEA
	protein binding	GO:0005515	IPI
	structural constituent of skin epidermis	GO:0030280	IDA
	transition metal ion binding	GO:0046914	IEA
Biological Process
	peptide cross-linking	GO:0018149	IDA
	keratinocyte differentiation	GO:0030216	TAS
	establishment of skin barrier	GO:0061436	IBA
	establishment of skin barrier	GO:0061436	IEP
Subcellular Localization
	cornified envelope	GO:0001533	IBA
	cornified envelope	GO:0001533	IDA
	nucleus	GO:0005634	HDA
	cytosol	GO:0005829	TAS
	keratohyalin granule	GO:0036457	IBA
	keratohyalin granule	GO:0036457	IDA
	cytoplasmic ribonucleoprotein granule	GO:0036464	IDA
	collagen-containing extracellular matrix	GO:0062023	HDA

Experiment description of studies that identified FLG in exosomes

Experiment ID

494

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD151\|CD63\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Osteoarthritic cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

496

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Healthy cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for FLG

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

APTX

Affinity Capture-MS

Homo sapiens

PRSS16

10279

Affinity Capture-MS

Homo sapiens

KRT18

3875

Cross-Linking-MS (XL-MS)

Homo sapiens

UBE2H

7328

Affinity Capture-MS

Homo sapiens

FBXW7

Affinity Capture-MS

Homo sapiens

GALNT9

Affinity Capture-MS

Homo sapiens

MRM1

Affinity Capture-MS

Homo sapiens

DTL

Affinity Capture-MS

Homo sapiens

STK16

8576

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

BLMH

642

Affinity Capture-MS

Homo sapiens

PPP2R2B

5521

Affinity Capture-MS

Homo sapiens

INPP5A

3632

Affinity Capture-MS

Homo sapiens

SLC25A6

293

Affinity Capture-MS

Homo sapiens

C18orf21

Affinity Capture-MS

Homo sapiens

MAK

Affinity Capture-MS

Homo sapiens

GMCL1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

METTL14

Affinity Capture-MS

Homo sapiens

CCNC

Affinity Capture-MS

Homo sapiens

ATG5

9474

Proximity Label-MS

Homo sapiens

FCF1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ANXA2

302

Affinity Capture-MS

Homo sapiens

USP1

7398

Affinity Capture-MS

Homo sapiens

SH2D3A

Affinity Capture-MS

Homo sapiens

TBC1D22B

Affinity Capture-MS

Homo sapiens

MCL1

4170

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SLC35F2

54733

Affinity Capture-MS

Homo sapiens

TUBG1

7283

Affinity Capture-MS

Homo sapiens

UCP2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ZSCAN12

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ERCC3

Affinity Capture-MS

Homo sapiens

C1orf35

Affinity Capture-MS

Homo sapiens

ERP27

Affinity Capture-MS

Homo sapiens

NPHP1

Affinity Capture-MS

Homo sapiens

ST6GALNAC6

30815

Affinity Capture-MS

Homo sapiens

ZC3HC1

Affinity Capture-MS

Homo sapiens

PTDSS1

9791

Affinity Capture-MS

Homo sapiens

HNRNPA1

3178

Affinity Capture-MS

Homo sapiens

LRRC10

Affinity Capture-MS

Homo sapiens

FTH1

2495

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CASP14

23581

Affinity Capture-MS

Homo sapiens

PKP1

5317

Affinity Capture-MS

Homo sapiens

FNDC5

Affinity Capture-MS

Homo sapiens

PI4KA

5297

Affinity Capture-MS

Homo sapiens

C21orf59

56683

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PSMD14

10213

Affinity Capture-MS

Homo sapiens

KLF5

Affinity Capture-MS

Homo sapiens

E2F6

1876

Affinity Capture-MS

Homo sapiens

DPPA4

Affinity Capture-MS

Homo sapiens

FRMD1

Affinity Capture-MS

Homo sapiens

KIR3DS1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PI4KAP1

Affinity Capture-MS

Homo sapiens

EZH2

Affinity Capture-MS

Homo sapiens

TGM3

7053

Affinity Capture-MS

Homo sapiens

TMEM79

Affinity Capture-MS

Homo sapiens

TRIM21

6737

Affinity Capture-MS

Homo sapiens

KIF23

9493

Affinity Capture-MS

Homo sapiens

PINK1

Affinity Capture-MS

Homo sapiens

GPR18

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GOT1

2805

Affinity Capture-MS

Homo sapiens

SARAF

Affinity Capture-MS

Homo sapiens

DSG1

1828

Affinity Capture-MS

Homo sapiens

STK11

6794

Affinity Capture-MS

Homo sapiens

ACAD9

28976

Affinity Capture-MS

Homo sapiens

TXNIP

10628

Affinity Capture-MS

Homo sapiens

ALDH3A1

218

Affinity Capture-MS

Homo sapiens

ZDHHC19

Affinity Capture-MS

Homo sapiens

B3GNT2

10678

Affinity Capture-MS

Homo sapiens

OR2A4

79541

Affinity Capture-MS

Homo sapiens

TNFRSF19

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

OR13C3

Affinity Capture-MS

Homo sapiens

CUL2

8453

Affinity Capture-MS

Homo sapiens

DDX3X

1654

Affinity Capture-MS

Homo sapiens

AQP12B

Affinity Capture-MS

Homo sapiens

CCR1

Affinity Capture-MS

Homo sapiens

ATP6AP2

10159

Affinity Capture-MS

Homo sapiens

SCN3B

Affinity Capture-MS

Homo sapiens

FIBIN

Affinity Capture-MS

Homo sapiens

STX17

55014

Affinity Capture-MS

Homo sapiens

P2RX6

Affinity Capture-MS

Homo sapiens

RIPPLY3

Affinity Capture-MS

Homo sapiens

CYLD

Affinity Capture-MS

Homo sapiens

MECP2

4204

Affinity Capture-MS

Homo sapiens

MLLT6

Affinity Capture-MS

Homo sapiens

UCHL5

51377

Reconstituted Complex

Homo sapiens

FLG2

388698

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which FLG is involved

Pathway	Evidence	Source
Developmental Biology	TAS	Reactome
Developmental Cell Lineages	TAS	Reactome
Differentiation of keratinocytes in interfollicular epidermis in mammalian skin	TAS	Reactome
Formation of the cornified envelope	TAS	Reactome
Keratinization	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here