|
 Gene ontology annotations for COPZ1 |
|
|
| Experiment description of studies that identified COPZ1 in exosomes |
| 1 |
| Experiment ID |
207 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|HSP70
|
EV Cytosolic markers |
|
✔
FLOT1
|
EV Membrane markers |
|
✔
VDAC
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
DKO-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 2 |
| Experiment ID |
208 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|HSP70
|
EV Cytosolic markers |
|
✔
FLOT1
|
EV Membrane markers |
|
✔
VDAC
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
Dks-8 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 3 |
| Experiment ID |
209 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
TSG101|HSP70
|
EV Cytosolic markers |
|
✔
FLOT1
|
EV Membrane markers |
|
✔
VDAC
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ. |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
DLD-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 4 |
| Experiment ID |
236 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
EV Cytosolic markers |
|
✘
|
EV Membrane markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✔
qNano
|
EV Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
RNA Sequencing
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocellular carcinoma cells |
| Sample name |
MHCC97L |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing |
|
|
| 5 |
| Experiment ID |
191 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
Alix
|
EV Cytosolic markers |
|
✔
CD81|CD9
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
20124223
|
| Organism |
Homo sapiens |
| Experiment description |
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. |
| Authors |
Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK. |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2010 |
| Sample |
Squamous carcinoma cells |
| Sample name |
Squamous carcinoma cell (A431) |
| Isolation/purification methods |
Differential centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 6 |
| Experiment ID |
217 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101
|
EV Cytosolic markers |
|
✔
CD81|CD9|CD63
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23844026
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of human thymic exosomes. |
| Authors |
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. |
| Journal name |
PLoS One
|
| Publication year |
2013 |
| Sample |
Thymus |
| Sample name |
Normal-Thymus |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| Protein-protein interactions for COPZ1 |
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| Pathways in which COPZ1 is involved |
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