Gene description for DNAJB8
Gene name DnaJ (Hsp40) homolog, subfamily B, member 8
Gene symbol DNAJB8
Other names/aliases CT156
DJ6
Species Homo sapiens
 Database cross references - DNAJB8
ExoCarta ExoCarta_165721
Vesiclepedia VP_165721
Entrez Gene 165721
HGNC 23699
MIM 611337
UniProt Q8NHS0  
 DNAJB8 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 35165262    
Colon cancer cells 35165262    
Colon cancer cells 35165262    
Colon cancer cells 35165262    
 Gene ontology annotations for DNAJB8
Molecular Function
    Hsp70 protein binding GO:0030544 IEA
    protein folding chaperone GO:0044183 IBA
    protein folding chaperone GO:0044183 IDA
    unfolded protein binding GO:0051082 IBA
    unfolded protein binding GO:0051082 IDA
    protein-folding chaperone binding GO:0051087 IBA
    protein-folding chaperone binding GO:0051087 IPI
Biological Process
    chaperone-mediated protein folding GO:0061077 IBA
    chaperone-mediated protein folding GO:0061077 IDA
    negative regulation of inclusion body assembly GO:0090084 IDA
Subcellular Localization
    nucleus GO:0005634 HDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified DNAJB8 in sEVs
1
Experiment ID 1265
MISEV standards
Biophysical techniques
CD63|CD81|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name SW480
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 1266
MISEV standards
Biophysical techniques
CD63|CD81|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name SW480 - Oxaliplatin-resistant
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
3
Experiment ID 1267
MISEV standards
Biophysical techniques
CD63|CD81|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name SW620
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 1268
MISEV standards
Biophysical techniques
CD63|CD81|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 35165262    
Organism Homo sapiens
Experiment description DNAJB8 in small extracellular vesicles promotes Oxaliplatin resistance through TP53/MDR1 pathway in colon cancer
Authors "Wang Z, Li Y, Mao R, Zhang Y, Wen J, Liu Q, Liu Y, Zhang T."
Journal name Cell Death Dis
Publication year 2022
Sample Colon cancer cells
Sample name SW620 - Oxaliplatin-resistant
Isolation/purification methods Differential centrifugation
Tangential flow filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for DNAJB8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STUB1 10273
Affinity Capture-MS Homo sapiens
2 DNAJC7 7266
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
3 IDI2  
Affinity Capture-MS Homo sapiens
4 KIAA0100  
Affinity Capture-MS Homo sapiens
5 SUGT1 10910
Affinity Capture-MS Homo sapiens
6 PI4KA 5297
Affinity Capture-MS Homo sapiens
7 HDAC4  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 FAR1 84188
Affinity Capture-MS Homo sapiens
10 DGCR14  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TTC26 79989
Proximity Label-MS Homo sapiens
13 RPL34 6164
Proximity Label-MS Homo sapiens
14 AIFM1 9131
Affinity Capture-MS Homo sapiens
15 HSPA6 3310
Affinity Capture-Western Homo sapiens
16 NDUFB10 4716
Affinity Capture-MS Homo sapiens
17 DNAJB12 54788
Affinity Capture-MS Homo sapiens
18 FDFT1 2222
Affinity Capture-MS Homo sapiens
19 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NDUFA9 4704
Affinity Capture-MS Homo sapiens
21 BAG2 9532
Affinity Capture-MS Homo sapiens
22 USP34 9736
Affinity Capture-MS Homo sapiens
23 BAG5 9529
Affinity Capture-MS Homo sapiens
24 HDAC6 10013
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 CDK5RAP2 55755
Proximity Label-MS Homo sapiens
26 DNAJB2 3300
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 KEAP1 9817
Affinity Capture-MS Homo sapiens
28 ASPM 259266
Affinity Capture-MS Homo sapiens
29 PACSIN2 11252
Proximity Label-MS Homo sapiens
30 HSPA1L 3305
Proximity Label-MS Homo sapiens
31 EMD 2010
Affinity Capture-MS Homo sapiens
32 RNF219  
Affinity Capture-MS Homo sapiens
33 GEMIN8 54960
Proximity Label-MS Homo sapiens
34 C9orf40  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 FKBP8 23770
Affinity Capture-MS Homo sapiens
36 TMEM263 90488
Affinity Capture-MS Homo sapiens
37 CAPRIN1 4076
Affinity Capture-MS Homo sapiens
38 PGAM5 192111
Affinity Capture-MS Homo sapiens
39 GRPEL1 80273
Affinity Capture-MS Homo sapiens
40 MAMDC2 256691
Affinity Capture-MS Homo sapiens
41 CHCHD3 54927
Affinity Capture-MS Homo sapiens
42 TUBB6 84617
Affinity Capture-MS Homo sapiens
43 TIMM50 92609
Affinity Capture-MS Homo sapiens
44 THAP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 AKAP8  
Affinity Capture-MS Homo sapiens
46 MLF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 FKBP5 2289
Affinity Capture-MS Homo sapiens
48 MLLT11  
Affinity Capture-MS Homo sapiens
49 NDUFA5 4698
Affinity Capture-MS Homo sapiens
50 ESRRB  
Affinity Capture-MS Homo sapiens
51 ERF  
Affinity Capture-MS Homo sapiens
52 YME1L1 10730
Affinity Capture-MS Homo sapiens
53 LRRK2 120892
Affinity Capture-Western Homo sapiens
54 HSPA2 3306
Affinity Capture-MS Homo sapiens
55 RGPD5  
Affinity Capture-MS Homo sapiens
56 DNAJB6 10049
Affinity Capture-MS Homo sapiens
57 NACC2  
Affinity Capture-MS Homo sapiens
58 SMARCAD1  
Proximity Label-MS Homo sapiens
59 HSPA1A 3303
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
60 HSPA4 3308
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
61 CBX6  
Affinity Capture-MS Homo sapiens
62 SIRT2 22933
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
63 ZBTB20 26137
Affinity Capture-MS Homo sapiens
64 NDUFAF4 29078
Affinity Capture-MS Homo sapiens
65 LCN1 3933
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 TUBA1C 84790
Affinity Capture-MS Homo sapiens
67 SEH1L 81929
Proximity Label-MS Homo sapiens
68 HAX1  
Affinity Capture-MS Homo sapiens
69 FAM83H 286077
Affinity Capture-MS Homo sapiens
70 HSPA9 3313
Affinity Capture-MS Homo sapiens
71 DNAJB1 3337
Affinity Capture-MS Homo sapiens
72 NOL9 79707
Proximity Label-MS Homo sapiens
73 STIP1 10963
Proximity Label-MS Homo sapiens
74 PFDN5 5204
Proximity Label-MS Homo sapiens
75 HERC2 8924
Proximity Label-MS Homo sapiens
76 SCO2  
Affinity Capture-MS Homo sapiens
77 HLA-C 3107
Affinity Capture-MS Homo sapiens
78 CDC123 8872
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DNAJB8 is involved
No pathways found





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