Gene description for GRB10
Gene name growth factor receptor-bound protein 10
Gene symbol GRB10
Other names/aliases GRB-IR
Grb-10
IRBP
MEG1
RSS
Species Homo sapiens
 Database cross references - GRB10
ExoCarta ExoCarta_2887
Vesiclepedia VP_2887
Entrez Gene 2887
HGNC 4564
MIM 601523
UniProt Q13322  
 GRB10 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for GRB10
Molecular Function
    insulin receptor binding GO:0005158 IBA
    insulin receptor binding GO:0005158 ISS
    protein binding GO:0005515 IPI
    signaling receptor complex adaptor activity GO:0030159 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    insulin receptor signaling pathway GO:0008286 IBA
    insulin receptor signaling pathway GO:0008286 ISS
    gene expression GO:0010467 IEA
    negative regulation of Wnt signaling pathway GO:0030178 IBA
    negative regulation of Wnt signaling pathway GO:0030178 IDA
    positive regulation of vascular endothelial growth factor receptor signaling pathway GO:0030949 IDA
    response to insulin GO:0032868 ISS
    positive regulation of phosphorylation GO:0042327 ISS
    negative regulation of glycogen biosynthetic process GO:0045719 ISS
    negative regulation of D-glucose import GO:0046325 ISS
    negative regulation of insulin receptor signaling pathway GO:0046627 IBA
    negative regulation of insulin receptor signaling pathway GO:0046627 ISS
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    ERK1 and ERK2 cascade GO:0070371 IEA
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
    vascular associated smooth muscle cell migration GO:1904738 IEA
Subcellular Localization
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified GRB10 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for GRB10
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 ZMYND19  
Two-hybrid Homo sapiens
3 NRN1  
Affinity Capture-MS Homo sapiens
4 CHFR  
Reconstituted Complex Homo sapiens
5 GIGYF2 26058
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
6 KPNA3 3839
Two-hybrid Homo sapiens
7 ERBB2 2064
Reconstituted Complex Homo sapiens
8 NEDD4 4734
Affinity Capture-Western Homo sapiens
9 MAGEA3  
Affinity Capture-MS Homo sapiens
10 RILP 83547
Affinity Capture-MS Homo sapiens
11 DENND5B  
Co-fractionation Homo sapiens
12 CCNYL1 151195
Affinity Capture-MS Homo sapiens
13 BLK 640
Two-hybrid Homo sapiens
14 SHANK2 22941
Affinity Capture-Western Homo sapiens
15 ERBB4 2066
Reconstituted Complex Homo sapiens
16 YWHAE 7531
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
17 ZDHHC17 23390
Two-hybrid Homo sapiens
18 IQCB1  
Affinity Capture-MS Homo sapiens
19 RQCD1 9125
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
20 KIT 3815
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
21 YWHAZ 7534
Affinity Capture-MS Homo sapiens
22 EPHB1 2047
Two-hybrid Homo sapiens
23 ABL1 25
Reconstituted Complex Homo sapiens
24 INSR 3643
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
25 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
Reconstituted Complex Homo sapiens
26 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
27 GRB10 2887
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 RET 5979
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
29 IGF1R 3480
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
30 DDX58 23586
Affinity Capture-RNA Homo sapiens
31 MKI67  
Affinity Capture-MS Homo sapiens
32 MAP2K1 5604
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
33 APP 351
Reconstituted Complex Homo sapiens
34 MEGF10 84466
Two-hybrid Homo sapiens
35 TRIM55  
Two-hybrid Homo sapiens
36 NFKBID  
Two-hybrid Homo sapiens
37 XPO1 7514
Affinity Capture-MS Homo sapiens
38 NOA1  
Two-hybrid Homo sapiens
39 KDR  
Two-hybrid Homo sapiens
40 CCNB1 891
Negative Genetic Homo sapiens
41 AANAT  
Two-hybrid Homo sapiens
42 TNK1  
Two-hybrid Homo sapiens
43 FYN 2534
Biochemical Activity Homo sapiens
44 HECW2  
Affinity Capture-MS Homo sapiens
45 GIGYF1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
46 FARS2 10667
Two-hybrid Homo sapiens
47 ENTHD2  
Two-hybrid Homo sapiens
48 KANK2 25959
Two-hybrid Homo sapiens
49 TEKT4  
Two-hybrid Homo sapiens
50 WDR59 79726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 RAF1 5894
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
52 GHR  
Affinity Capture-Western Homo sapiens
53 SRC 6714
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
54 PIK3R1 5295
Affinity Capture-Western Homo sapiens
55 AHNAK2 113146
Affinity Capture-MS Homo sapiens
56 YWHAG 7532
Affinity Capture-MS Homo sapiens
57 NARS 4677
Co-fractionation Homo sapiens
58 AKT1 207
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
59 JAK2 3717
Affinity Capture-Western Homo sapiens
60 IRS1 3667
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
61 PPP2R2D 55844
Affinity Capture-MS Homo sapiens
62 S100A2 6273
Affinity Capture-MS Homo sapiens
63 NEDD4L 23327
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
64 TRIM63  
Two-hybrid Homo sapiens
65 IRS2 8660
Affinity Capture-Western Homo sapiens
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