Gene description for PSEN2
Gene name presenilin 2
Gene symbol PSEN2
Other names/aliases AD3L
AD4
CMD1V
PS2
STM2
Species Homo sapiens
 Database cross references - PSEN2
ExoCarta ExoCarta_5664
Vesiclepedia VP_5664
Entrez Gene 5664
HGNC 9509
MIM 600759
UniProt P49810  
 PSEN2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PSEN2
Molecular Function
    protein binding GO:0005515 IPI
    aspartic endopeptidase activity, intramembrane cleaving GO:0042500 IBA
Biological Process
    response to hypoxia GO:0001666 IEA
    membrane protein ectodomain proteolysis GO:0006509 IBA
    membrane protein ectodomain proteolysis GO:0006509 IDA
    Notch signaling pathway GO:0007219 IBA
    Notch receptor processing GO:0007220 TAS
    protein processing GO:0016485 IBA
    protein processing GO:0016485 IDA
    amyloid-beta formation GO:0034205 IBA
    amyloid-beta formation GO:0034205 IDA
    intracellular signal transduction GO:0035556 IEA
    amyloid precursor protein catabolic process GO:0042987 IDA
    amyloid precursor protein catabolic process GO:0042987 TAS
    calcium ion homeostasis GO:0055074 IBA
    regulation of calcium import into the mitochondrion GO:0110097 IMP
    mitochondrion-endoplasmic reticulum membrane tethering GO:1990456 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    kinetochore GO:0000776 IDA
    nuclear inner membrane GO:0005637 IDA
    early endosome GO:0005769 IEA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IDA
    centrosome GO:0005813 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    synaptic vesicle GO:0008021 IEA
    membrane GO:0016020 HDA
    protein-containing complex GO:0032991 IDA
    presynaptic membrane GO:0042734 IEA
    gamma-secretase complex GO:0070765 IBA
    gamma-secretase complex GO:0070765 IDA
 Experiment description of studies that identified PSEN2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PSEN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FBXL12 54850
Two-hybrid Homo sapiens
2 APP 351
Two-hybrid Homo sapiens
3 TSPAN5 10098
Affinity Capture-MS Homo sapiens
4 ART3  
Affinity Capture-MS Homo sapiens
5 NPLOC4 55666
Affinity Capture-MS Homo sapiens
6 FAF2 23197
Affinity Capture-MS Homo sapiens
7 HPS1  
Two-hybrid Homo sapiens
8 PSEN1 5663
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 NCSTN 23385
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 CLK1  
Affinity Capture-MS Homo sapiens
11 RNF115  
Affinity Capture-MS Homo sapiens
12 ECD  
Two-hybrid Homo sapiens
13 PSENEN  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 AAMP 14
Two-hybrid Homo sapiens
15 NOS3  
Two-hybrid Homo sapiens
16 GCDH 2639
Two-hybrid Homo sapiens
17 GFAP 2670
Two-hybrid Homo sapiens
18 ERGIC3 51614
Affinity Capture-MS Homo sapiens
19 SPC24 147841
Two-hybrid Homo sapiens
20 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 PPP5C 5536
Affinity Capture-MS Homo sapiens
22 APBA3  
Affinity Capture-Western Homo sapiens
23 CEP55 55165
Two-hybrid Homo sapiens
24 RNF32  
Two-hybrid Homo sapiens
25 ELAVL1 1994
Two-hybrid Homo sapiens
26 IFIT5 24138
Two-hybrid Homo sapiens
27 DLK1  
Affinity Capture-MS Homo sapiens
28 CHKA 1119
Two-hybrid Homo sapiens
29 KCNIP3  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 SRI 6717
Affinity Capture-Western Homo sapiens
31 DYNC1H1 1778
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
32 PDCD4 27250
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
33 ACTG1 71
Two-hybrid Homo sapiens
34 RHEB 6009
Two-hybrid Homo sapiens
35 BCL2L1 598
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
36 KCNIP4 80333
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
37 KIF22  
Affinity Capture-MS Homo sapiens
38 APBA2 321
Affinity Capture-Western Homo sapiens
39 APH1B 83464
Affinity Capture-Western Homo sapiens
40 EXOC6 54536
Two-hybrid Homo sapiens
41 FLNB 2317
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
42 CIB1 10519
Reconstituted Complex Homo sapiens
Phenotypic Enhancement Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
43 DNAJB1 3337
Two-hybrid Homo sapiens
44 EID1  
Two-hybrid Homo sapiens
45 APH1A 51107
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
46 ALG5 29880
Affinity Capture-MS Homo sapiens
47 IFIT3 3437
Two-hybrid Homo sapiens
48 IRF3 3661
Affinity Capture-MS Homo sapiens
49 CAPN1 823
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
50 UBQLN1 29979
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
51 MAST1  
Two-hybrid Homo sapiens
52 ICAM5 7087
Reconstituted Complex Homo sapiens
53 FHL2 2274
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
54 APBA1  
Affinity Capture-Western Homo sapiens
55 PSEN2 5664
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
56 MGEA5 10724
Two-hybrid Homo sapiens
57 ECSIT 51295
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
58 LIPF  
Two-hybrid Homo sapiens
59 EVI5L  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PSEN2 is involved
PathwayEvidenceSource
Activated NOTCH1 Transmits Signal to the Nucleus IEA Reactome
Axon guidance TAS Reactome
Cell death signalling via NRAGE, NRIF and NADE TAS Reactome
Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants TAS Reactome
Constitutive Signaling by NOTCH1 PEST Domain Mutants TAS Reactome
Death Receptor Signaling TAS Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
EPH-ephrin mediated repulsion of cells TAS Reactome
EPH-Ephrin signaling TAS Reactome
Nervous system development TAS Reactome
Noncanonical activation of NOTCH3 TAS Reactome
NOTCH2 Activation and Transmission of Signal to the Nucleus IEA Reactome
NOTCH3 Activation and Transmission of Signal to the Nucleus TAS Reactome
NOTCH4 Activation and Transmission of Signal to the Nucleus IEA Reactome
NRIF signals cell death from the nucleus TAS Reactome
Nuclear signaling by ERBB4 TAS Reactome
p75 NTR receptor-mediated signalling TAS Reactome
Regulated proteolysis of p75NTR TAS Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by ERBB4 TAS Reactome
Signaling by NOTCH IEA Reactome
Signaling by NOTCH TAS Reactome
Signaling by NOTCH1 IEA Reactome
Signaling by NOTCH1 HD+PEST Domain Mutants in Cancer TAS Reactome
Signaling by NOTCH1 in Cancer TAS Reactome
Signaling by NOTCH1 PEST Domain Mutants in Cancer TAS Reactome
Signaling by NOTCH2 IEA Reactome
Signaling by NOTCH3 TAS Reactome
Signaling by NOTCH4 IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by TGFB family members TAS Reactome
Signaling by TGFBR3 TAS Reactome
TGFBR3 PTM regulation TAS Reactome





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