Gene description for KRT27
Gene name keratin 27, type I
Gene symbol KRT27
Other names/aliases KRT25C
Species Homo sapiens
 Database cross references - KRT27
ExoCarta ExoCarta_342574
Vesiclepedia VP_342574
Entrez Gene 342574
HGNC 30841
UniProt Q7Z3Y8  
 KRT27 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 19837982    
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for KRT27
Molecular Function
    structural molecule activity GO:0005198 IEA
    protein binding GO:0005515 IPI
Biological Process
    epithelial cell differentiation GO:0030855 IBA
    hair follicle morphogenesis GO:0031069 IBA
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IBA
    intermediate filament GO:0005882 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KRT27 in exosomes
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KRT27
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT76 51350
Two-hybrid Homo sapiens
2 KRT77 374454
Two-hybrid Homo sapiens
3 SUFU 51684
Two-hybrid Homo sapiens
4 SLIRP 81892
Two-hybrid Homo sapiens
5 CENPH  
Affinity Capture-MS Homo sapiens
6 DPPA3  
Two-hybrid Homo sapiens
7 CCDC88B 283234
Affinity Capture-MS Homo sapiens
8 KRT6B 3854
Two-hybrid Homo sapiens
9 KRT1 3848
Two-hybrid Homo sapiens
10 ATG5 9474
Affinity Capture-MS Homo sapiens
11 NFE2L1  
Affinity Capture-MS Homo sapiens
12 SCNM1  
Two-hybrid Homo sapiens
13 ZC3H14  
Two-hybrid Homo sapiens
14 GFAP 2670
Two-hybrid Homo sapiens
15 NUPL1 9818
Two-hybrid Homo sapiens
16 NAV1 89796
Affinity Capture-MS Homo sapiens
17 KRT4 3851
Two-hybrid Homo sapiens
18 GAS8  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
19 BFSP2  
Two-hybrid Homo sapiens
20 ZNF80  
Two-hybrid Homo sapiens
21 TTC23  
Two-hybrid Homo sapiens
22 VPS51 738
Affinity Capture-MS Homo sapiens
23 PBX3  
Two-hybrid Homo sapiens
24 MTMR9 66036
Two-hybrid Homo sapiens
25 HAPLN2  
Two-hybrid Homo sapiens
26 RTP5  
Two-hybrid Homo sapiens
27 ZNF3  
Two-hybrid Homo sapiens
28 HOXB9  
Two-hybrid Homo sapiens
29 BLOC1S1  
Affinity Capture-MS Homo sapiens
30 SPDL1  
Affinity Capture-MS Homo sapiens
31 INA 9118
Affinity Capture-MS Homo sapiens
32 SNAPIN 23557
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
33 LKAAEAR1  
Two-hybrid Homo sapiens
34 CEP95  
Two-hybrid Homo sapiens
35 GOLGA3 2802
Affinity Capture-MS Homo sapiens
36 RASAL3 64926
Two-hybrid Homo sapiens
37 PPP1R18 170954
Two-hybrid Homo sapiens
38 CCDC138  
Two-hybrid Homo sapiens
39 POLL  
Two-hybrid Homo sapiens
40 HGS 9146
Two-hybrid Homo sapiens
41 CCDC53 51019
Two-hybrid Homo sapiens
42 OIP5  
Two-hybrid Homo sapiens
43 SEMA4C 54910
Two-hybrid Homo sapiens
44 KRT5 3852
Two-hybrid Homo sapiens
45 MBD3L2  
Two-hybrid Homo sapiens
46 KRT78 196374
Two-hybrid Homo sapiens
47 USP33  
Affinity Capture-MS Homo sapiens
48 KRT72 140807
Two-hybrid Homo sapiens
49 KRT83  
Two-hybrid Homo sapiens
50 SERPINA1 5265
Affinity Capture-MS Homo sapiens
51 HAUS1  
Affinity Capture-MS Homo sapiens
52 MGC50722  
Two-hybrid Homo sapiens
53 LOH12CR1 118426
Affinity Capture-MS Homo sapiens
54 CCDC185  
Two-hybrid Homo sapiens
55 RCOR3  
Two-hybrid Homo sapiens
56 HAUS4  
Affinity Capture-MS Homo sapiens
57 C1orf216  
Two-hybrid Homo sapiens
58 AIF1  
Affinity Capture-MS Homo sapiens
59 XRCC4  
Two-hybrid Homo sapiens
60 HSPBP1 23640
Two-hybrid Homo sapiens
61 KRT8 3856
Two-hybrid Homo sapiens
62 KIAA1217 56243
Two-hybrid Homo sapiens
63 FMNL2 114793
Two-hybrid Homo sapiens
64 C10orf32  
Affinity Capture-MS Homo sapiens
65 C19orf44  
Two-hybrid Homo sapiens
66 BEX2  
Two-hybrid Homo sapiens
67 TRIML2  
Two-hybrid Homo sapiens
68 NDUFAF3 25915
Two-hybrid Homo sapiens
69 CEP55 55165
Affinity Capture-MS Homo sapiens
70 PRPH 5630
Two-hybrid Homo sapiens
71 MOS 4342
Two-hybrid Homo sapiens
72 RNF39  
Two-hybrid Homo sapiens
73 FAM110A  
Two-hybrid Homo sapiens
74 RABEP1 9135
Affinity Capture-MS Homo sapiens
75 KRT6A 3853
Two-hybrid Homo sapiens
76 EXOC4 60412
Affinity Capture-MS Homo sapiens
77 ATG16L1 55054
Affinity Capture-MS Homo sapiens
78 FAM107A  
Two-hybrid Homo sapiens
79 DTNBP1  
Affinity Capture-MS Homo sapiens
80 BLOC1S5 63915
Affinity Capture-MS Homo sapiens
81 TXLNG  
Affinity Capture-MS Homo sapiens
82 SPAG7  
Affinity Capture-MS Homo sapiens
83 WDYHV1  
Two-hybrid Homo sapiens
84 DTNB 1838
Two-hybrid Homo sapiens
85 GSTK1 373156
Two-hybrid Homo sapiens
86 SYNC  
Affinity Capture-MS Homo sapiens
87 KRT75 9119
Two-hybrid Homo sapiens
88 ZNF580  
Two-hybrid Homo sapiens
89 CCDC101  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
90 ARHGEF6 9459
Two-hybrid Homo sapiens
91 CCDC22 28952
Two-hybrid Homo sapiens
92 KRT2 3849
Two-hybrid Homo sapiens
93 ZNF581  
Two-hybrid Homo sapiens
94 CUL2 8453
Affinity Capture-MS Homo sapiens
95 COG5 10466
Affinity Capture-MS Homo sapiens
96 TSC1 7248
Affinity Capture-MS Homo sapiens
97 C16orf59 80178
Two-hybrid Homo sapiens
98 TXLNB  
Two-hybrid Homo sapiens
99 CYLD  
Affinity Capture-MS Homo sapiens
100 GPS2  
Two-hybrid Homo sapiens
101 SMARCC2 6601
Two-hybrid Homo sapiens
102 KRT85  
Two-hybrid Homo sapiens
103 SDHAF1  
Two-hybrid Homo sapiens
104 GAN 8139
Affinity Capture-MS Homo sapiens
105 KRT3 3850
Two-hybrid Homo sapiens
106 COX5B 1329
Two-hybrid Homo sapiens
107 LMNTD2  
Two-hybrid Homo sapiens
108 CCNC  
Two-hybrid Homo sapiens
109 KRT86  
Two-hybrid Homo sapiens
110 PNN 5411
Two-hybrid Homo sapiens
111 CCDC186 55088
Affinity Capture-MS Homo sapiens
112 KLHL38  
Two-hybrid Homo sapiens
113 SSX3  
Two-hybrid Homo sapiens
114 EPPK1 83481
Affinity Capture-MS Homo sapiens
115 SLC22A5 6584
Two-hybrid Homo sapiens
116 DUSP26 78986
Two-hybrid Homo sapiens
117 PRKD1 5587
Affinity Capture-MS Homo sapiens
118 HOOK3 84376
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
119 GADD45GIP1  
Two-hybrid Homo sapiens
120 KRT82 3888
Two-hybrid Homo sapiens
121 SASS6 163786
Affinity Capture-MS Homo sapiens
122 TNIP2  
Affinity Capture-MS Homo sapiens
123 ARFIP1 27236
Two-hybrid Homo sapiens
124 CCDC151  
Two-hybrid Homo sapiens
125 HAUS5 23354
Affinity Capture-MS Homo sapiens
126 KRT81  
Two-hybrid Homo sapiens
127 MORN4  
Two-hybrid Homo sapiens
128 NEFL 4747
Affinity Capture-MS Homo sapiens
129 NEFM 4741
Affinity Capture-MS Homo sapiens
130 GOLGA1  
Two-hybrid Homo sapiens
131 PHF21A  
Affinity Capture-MS Homo sapiens
132 CGN  
Affinity Capture-MS Homo sapiens
133 MATR3 9782
Two-hybrid Homo sapiens
134 USHBP1  
Two-hybrid Homo sapiens
135 AMOT  
Two-hybrid Homo sapiens
136 L1TD1  
Affinity Capture-MS Homo sapiens
137 HAUS3  
Affinity Capture-MS Homo sapiens
138 TNNT1  
Two-hybrid Homo sapiens
139 TXLNA 200081
Affinity Capture-MS Homo sapiens
140 ZNF669  
Two-hybrid Homo sapiens
141 NUP54 53371
Two-hybrid Homo sapiens
142 GNG13  
Two-hybrid Homo sapiens
143 CCDC68  
Two-hybrid Homo sapiens
144 BCAS2 10286
Two-hybrid Homo sapiens
145 BLOC1S2 282991
Affinity Capture-MS Homo sapiens
146 C21orf58  
Two-hybrid Homo sapiens
147 VPS37C 55048
Two-hybrid Homo sapiens
148 CCHCR1  
Two-hybrid Homo sapiens
149 TEX28  
Two-hybrid Homo sapiens
150 CCDC146  
Two-hybrid Homo sapiens
151 BFSP1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
152 KLHDC3  
Affinity Capture-MS Homo sapiens
153 TSKS  
Two-hybrid Homo sapiens
154 KRT71 112802
Two-hybrid Homo sapiens
155 CEP250 11190
Affinity Capture-MS Homo sapiens
156 THAP7  
Two-hybrid Homo sapiens
157 KRT79 338785
Two-hybrid Homo sapiens
158 EXOC3L1  
Two-hybrid Homo sapiens
159 KRT6C 286887
Two-hybrid Homo sapiens
160 RNF38  
Affinity Capture-MS Homo sapiens
161 ABI2 10152
Two-hybrid Homo sapiens
162 PCDHB14  
Two-hybrid Homo sapiens
163 TBRG4 9238
Two-hybrid Homo sapiens
164 KRT20 54474
Two-hybrid Homo sapiens
165 KRT74 121391
Two-hybrid Homo sapiens
166 SPAG5 10615
Affinity Capture-MS Homo sapiens
167 TEAD4  
Two-hybrid Homo sapiens
168 AARD  
Two-hybrid Homo sapiens
169 KANK2 25959
Two-hybrid Homo sapiens
170 KXD1 79036
Affinity Capture-MS Homo sapiens
171 CFAP58 159686
Two-hybrid Homo sapiens
172 ICA1L  
Two-hybrid Homo sapiens
173 CCBE1 147372
Two-hybrid Homo sapiens
174 SSC5D 284297
Two-hybrid Homo sapiens
175 SUOX  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which KRT27 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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