Gene description for ERRFI1
Gene name ERBB receptor feedback inhibitor 1
Gene symbol ERRFI1
Other names/aliases GENE-33
MIG-6
MIG6
RALT
Species Homo sapiens
 Database cross references - ERRFI1
ExoCarta ExoCarta_54206
Entrez Gene 54206
HGNC 18185
MIM 608069
UniProt Q9UJM3  
 ERRFI1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for ERRFI1
Molecular Function
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    GTPase activator activity GO:0005096 TAS
Biological Process
    negative regulation of protein autophosphorylation GO:0031953 IDA
    lung vasculature development GO:0060426 ISS
    negative regulation of epidermal growth factor-activated receptor activity GO:0007175 ISS
    response to stress GO:0006950 TAS
    skin morphogenesis GO:0043589 ISS
    positive regulation of GTPase activity GO:0043547 TAS
    regulation of keratinocyte differentiation GO:0045616 ISS
    lung alveolus development GO:0048286 ISS
    lung epithelium development GO:0060428 ISS
Subcellular Localization
    cytoplasm GO:0005737 ISS
    nucleus GO:0005634 IEA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 ISS
 Experiment description of studies that identified ERRFI1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ERRFI1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDC42 998
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which ERRFI1 is involved
No pathways found





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