Gene description for SDHAF2
Gene name succinate dehydrogenase complex assembly factor 2
Gene symbol SDHAF2
Other names/aliases C11orf79
PGL2
SDH5
Species Homo sapiens
 Database cross references - SDHAF2
ExoCarta ExoCarta_54949
Entrez Gene 54949
HGNC 26034
MIM 613019
UniProt Q9NX18  
 SDHAF2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for SDHAF2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mitochondrial electron transport, succinate to ubiquinone GO:0006121 IMP
    protein dephosphorylation GO:0006470 IGI
    negative regulation of epithelial to mesenchymal transition GO:0010719 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 IGI
    protein-FAD linkage GO:0018293 IMP
Subcellular Localization
    mitochondrial matrix GO:0005759 IEA
    mitochondrion GO:0005739 IDA
 Experiment description of studies that identified SDHAF2 in exosomes
1
Experiment ID 234
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for SDHAF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MDS025  
Two-hybrid Homo sapiens
2 IMMT 10989
Two-hybrid Homo sapiens
3 EEF1A1 1915
Two-hybrid Homo sapiens
4 IKBKAP 8518
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SDHAF2 is involved
No pathways found





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