Gene description for LSM8
Gene name LSM8 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Gene symbol LSM8
Other names/aliases NAA38
Species Homo sapiens
 Database cross references - LSM8
ExoCarta ExoCarta_51691
Vesiclepedia VP_51691
Entrez Gene 51691
HGNC 20471
MIM 607288
UniProt O95777  
 LSM8 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for LSM8
Molecular Function
    RNA binding GO:0003723 HDA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    U6 snRNA binding GO:0017070 NAS
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IDA
    mRNA splicing, via spliceosome GO:0000398 NAS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 TAS
    U6 snRNP GO:0005688 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IBA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IDA
    U4/U6 x U5 tri-snRNP complex GO:0046540 IPI
    U2-type precatalytic spliceosome GO:0071005 IDA
    precatalytic spliceosome GO:0071011 IBA
    Lsm2-8 complex GO:0120115 IDA
 Experiment description of studies that identified LSM8 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LSM8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAB 7529
Co-fractionation Homo sapiens
2 MTHFR 4524
Two-hybrid Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 EXOSC6 118460
Two-hybrid Homo sapiens
5 EXOSC10 5394
Two-hybrid Homo sapiens
6 RBM5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
7 RPL32 6161
Affinity Capture-MS Homo sapiens
8 OLA1 29789
Affinity Capture-MS Homo sapiens
9 SEPT5 5413
Two-hybrid Homo sapiens
10 IGHMBP2 3508
Two-hybrid Homo sapiens
11 USP4 7375
Affinity Capture-MS Homo sapiens
12 SNRPE 6635
Affinity Capture-MS Homo sapiens
13 MTA2 9219
Co-fractionation Homo sapiens
14 Prpf8 192159
Affinity Capture-MS Mus musculus
15 THUMPD3 25917
Two-hybrid Homo sapiens
16 PDCD2 5134
Two-hybrid Homo sapiens
17 DCP1B  
Two-hybrid Homo sapiens
18 PHF11 51131
Two-hybrid Homo sapiens
19 USP15 9958
Affinity Capture-MS Homo sapiens
20 LSM2 57819
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
21 GTF2I 2969
Two-hybrid Homo sapiens
22 MGA  
Two-hybrid Homo sapiens
23 TALDO1 6888
Two-hybrid Homo sapiens
24 SNRPB 6628
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
25 ISOC2  
Two-hybrid Homo sapiens
26 ITSN2 50618
Two-hybrid Homo sapiens
27 ECHDC2 55268
Two-hybrid Homo sapiens
28 MAP4 4134
Co-fractionation Homo sapiens
29 DCP1A 55802
Two-hybrid Homo sapiens
30 LSM4 25804
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
31 DDX39A 10212
Co-fractionation Homo sapiens
32 EMD 2010
Affinity Capture-MS Homo sapiens
33 PRPF3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RPL24 6152
Two-hybrid Homo sapiens
35 HNRNPR 10236
Co-fractionation Homo sapiens
36 CPNE8 144402
Co-fractionation Homo sapiens
37 LSM5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
38 CSTF2 1478
Co-fractionation Homo sapiens
39 SMU1 55234
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
40 IFNLR1  
Two-hybrid Homo sapiens
41 RPL26L1 51121
Affinity Capture-MS Homo sapiens
42 FAM63A 55793
Two-hybrid Homo sapiens
43 EFTUD2 9343
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 PPIH 10465
Affinity Capture-MS Homo sapiens
45 SNRNP200 23020
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
46 LSM7  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
47 NME4 4833
Two-hybrid Homo sapiens
48 MRPL48  
Two-hybrid Homo sapiens
49 DBF4  
Two-hybrid Homo sapiens
50 ALYREF 10189
Affinity Capture-MS Homo sapiens
51 MYC  
Affinity Capture-MS Homo sapiens
52 SEPT10 151011
Two-hybrid Homo sapiens
53 LSM3 27258
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
54 GMPPA 29926
Two-hybrid Homo sapiens
55 UPF1 5976
Two-hybrid Homo sapiens
56 SNRPD1 6632
Two-hybrid Homo sapiens
57 USP39 10713
Affinity Capture-MS Homo sapiens
58 ARNT 405
Two-hybrid Homo sapiens
59 FERMT3 83706
Two-hybrid Homo sapiens
60 SART3 9733
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 SNRPN 6638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 LSM6 11157
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
63 PRPF4 9128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 KRAS 3845
Negative Genetic Homo sapiens
65 BCKDK 10295
Two-hybrid Homo sapiens
66 MPRIP 23164
Two-hybrid Homo sapiens
67 SNRPF 6636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 HSPA9 3313
Two-hybrid Homo sapiens
69 MYCN  
Affinity Capture-MS Homo sapiens
70 KCTD3  
Two-hybrid Homo sapiens
71 DPAGT1 1798
Two-hybrid Homo sapiens
72 GET4 51608
Co-fractionation Homo sapiens
73 PGD 5226
Two-hybrid Homo sapiens
74 RSC1A1  
Two-hybrid Homo sapiens
75 CDKN2A 1029
Co-fractionation Homo sapiens
76 SNRPD3 6634
Affinity Capture-MS Homo sapiens
77 COL23A1  
Two-hybrid Homo sapiens
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