Experiment description of studies that identified EGFL7 in sEVs

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

5 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for EGFL7

Protein Interactor ExoCarta ID Identification method PubMed Species 1 LY86   Affinity Capture-MS Homo sapiens 2 LRP3   Affinity Capture-MS Homo sapiens 3 UBR4 23352 Affinity Capture-MS Homo sapiens 4 CCL3   Affinity Capture-MS Homo sapiens 5 VWCE   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 RAB6B 51560 Affinity Capture-MS Homo sapiens 7 SPAG8   Two-hybrid Homo sapiens 8 HSPA5 3309 Affinity Capture-MS Homo sapiens 9 NOTCH3 4854 Two-hybrid Homo sapiens 10 GRN 2896 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 Notch2 18129 Affinity Capture-Western Mus musculus 12 LDLRAD1   Affinity Capture-MS Homo sapiens 13 GFI1B   Two-hybrid Homo sapiens 14 KIF14 9928 Affinity Capture-MS Homo sapiens 15 LIPH 200879 Affinity Capture-MS Homo sapiens 16 NOTCH2 4853 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 17 SMARCD1 6602 Two-hybrid Homo sapiens 18 NRSN1   Affinity Capture-MS Homo sapiens 19 LTBR 4055 Affinity Capture-MS Homo sapiens 20 C7orf34   Affinity Capture-MS Homo sapiens 21 HPN   Affinity Capture-MS Homo sapiens 22 AURKA 6790 Affinity Capture-MS Homo sapiens 23 ADAM33 80332 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 IFI30   Affinity Capture-MS Homo sapiens 25 PLAUR 5329 Affinity Capture-MS Homo sapiens 26 ASGR2   Affinity Capture-MS Homo sapiens 27 PRG2   Affinity Capture-MS Homo sapiens 28 CUL3 8452 Affinity Capture-MS Homo sapiens 29 KIF20A 10112 Affinity Capture-MS Homo sapiens 30 MFAP5   Affinity Capture-MS Homo sapiens 31 Cenpe   Affinity Capture-MS Mus musculus 32 VHL   Affinity Capture-MS Homo sapiens 33 HEXIM2   Two-hybrid Homo sapiens 34 UBR3   Affinity Capture-MS Homo sapiens 35 DLK1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 KLRG2 346689 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 37 HOXA1 3198 Two-hybrid Homo sapiens 38 GNE 10020 Two-hybrid Homo sapiens 39 RCN1 5954 Affinity Capture-MS Homo sapiens 40 A2M 2 Affinity Capture-MS Homo sapiens 41 FN1 2335 Affinity Capture-MS Homo sapiens 42 NELL1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 43 ADAM21   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 44 NOTCH4   Two-hybrid Homo sapiens 45 PROZ 8858 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 Notch1   Affinity Capture-Western Mus musculus 47 PLD3 23646 Affinity Capture-MS Homo sapiens 48 WDR76   Affinity Capture-MS Homo sapiens 49 Notch3   Affinity Capture-Western Mus musculus 50 ROCK2 9475 Affinity Capture-MS Homo sapiens 51 HLA-DPA1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 52 CRLF1 9244 Affinity Capture-MS Homo sapiens 53 RASSF10   Two-hybrid Homo sapiens 54 PIGT 51604 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 55 Eef1a1 13627 Affinity Capture-MS Mus musculus 56 OLFM2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 57 PNMA2   Affinity Capture-MS Homo sapiens 58 EGFL8   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 59 NELL2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 60 SLURP1   Affinity Capture-MS Homo sapiens 61 HGS 9146 Two-hybrid Homo sapiens 62 NOTCH1 4851 Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens 63 FBLN5 10516 Affinity Capture-MS Homo sapiens 64 CLTC 1213 Affinity Capture-MS Homo sapiens 65 KCMF1 56888 Affinity Capture-MS Homo sapiens 66 ST14 6768 Affinity Capture-MS Homo sapiens 67 SPINT2 10653 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 68 Notch4   Affinity Capture-Western Mus musculus 69 EFEMP1 2202 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 70 Smn1 20595 Affinity Capture-MS Mus musculus 71 DGCR2 9993 Affinity Capture-MS Homo sapiens 72 PLA2G10   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which EGFL7 is involved

No pathways found