Gene description for TIMP3
Gene name TIMP metallopeptidase inhibitor 3
Gene symbol TIMP3
Other names/aliases HSMRK222
K222
K222TA2
SFD
Species Homo sapiens
 Database cross references - TIMP3
ExoCarta ExoCarta_7078
Vesiclepedia VP_7078
Entrez Gene 7078
HGNC 11822
MIM 188826
UniProt P35625  
 TIMP3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TIMP3
Molecular Function
    protein binding GO:0005515 IPI
    metalloendopeptidase inhibitor activity GO:0008191 IBA
    metalloendopeptidase inhibitor activity GO:0008191 TAS
    metal ion binding GO:0046872 IEA
Biological Process
    visual perception GO:0007601 IEA
    response to hormone GO:0009725 IBA
    response to cytokine GO:0034097 IBA
    negative regulation of membrane protein ectodomain proteolysis GO:0051045 IBA
    negative regulation of membrane protein ectodomain proteolysis GO:0051045 IMP
    negative regulation of metalloendopeptidase activity GO:1904684 ISS
Subcellular Localization
    extracellular region GO:0005576 TAS
    basement membrane GO:0005604 IEA
    extracellular space GO:0005615 IBA
    nucleus GO:0005634 HDA
    extracellular matrix GO:0031012 IBA
    platelet dense granule lumen GO:0031089 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified TIMP3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
11
Experiment ID 260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
13
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TIMP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MMP2 4313
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 LY6H  
Affinity Capture-MS Homo sapiens
3 VWCE  
Affinity Capture-MS Homo sapiens
4 DKK3 27122
Affinity Capture-MS Homo sapiens
5 PPM1B 5495
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 MYCN  
Affinity Capture-MS Homo sapiens
8 COLEC11 78989
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 POGLUT1 56983
Affinity Capture-MS Homo sapiens
10 LRP1B 53353
Affinity Capture-MS Homo sapiens
11 CCL3  
Affinity Capture-MS Homo sapiens
12 DEFA1 1667
Affinity Capture-MS Homo sapiens
13 SORL1 6653
Affinity Capture-MS Homo sapiens
14 NOTCH2 4853
Affinity Capture-MS Homo sapiens
15 DDX3Y 8653
Affinity Capture-MS Homo sapiens
16 LDLRAD1  
Affinity Capture-MS Homo sapiens
17 PRKAR1B  
Affinity Capture-MS Homo sapiens
18 C7orf34  
Affinity Capture-MS Homo sapiens
19 HECTD3 79654
Affinity Capture-MS Homo sapiens
20 HPN  
Affinity Capture-MS Homo sapiens
21 LRP2 4036
Affinity Capture-MS Homo sapiens
22 AKAP11 11215
Affinity Capture-MS Homo sapiens
23 WFDC6  
Affinity Capture-MS Homo sapiens
24 IFI30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CDC42EP1 11135
Affinity Capture-MS Homo sapiens
26 PDGFRA 5156
Affinity Capture-MS Homo sapiens
27 ASGR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 COLEC10 10584
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 FREM2 341640
Affinity Capture-MS Homo sapiens
30 RLN1  
Affinity Capture-MS Homo sapiens
31 IGFBP1  
Affinity Capture-MS Homo sapiens
32 ADPGK 83440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CDH23 64072
Affinity Capture-MS Homo sapiens
34 KLRG2 346689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 SLURP1  
Affinity Capture-MS Homo sapiens
36 CELA3B  
Affinity Capture-MS Homo sapiens
37 PRKACB 5567
Affinity Capture-MS Homo sapiens
38 APP 351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 SEL1L 6400
Affinity Capture-MS Homo sapiens
40 NELL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ADAM17 6868
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
42 PRKACG  
Affinity Capture-MS Homo sapiens
43 UBR4 23352
Affinity Capture-MS Homo sapiens
44 COL6A1 1291
Affinity Capture-MS Homo sapiens
45 MSMB  
Affinity Capture-MS Homo sapiens
46 FUT11 170384
Affinity Capture-MS Homo sapiens
47 CCL3L1  
Affinity Capture-MS Homo sapiens
48 C1QTNF7  
Affinity Capture-MS Homo sapiens
49 CSN1S1 1446
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 LDLR 3949
Affinity Capture-MS Homo sapiens
51 MYC  
Affinity Capture-MS Homo sapiens
52 LRP5 4041
Affinity Capture-MS Homo sapiens
53 MMP3 4314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 ADAMTS7  
Affinity Capture-MS Homo sapiens
55 SCGB2A2  
Affinity Capture-MS Homo sapiens
56 CBWD1 55871
Affinity Capture-MS Homo sapiens
57 DEFA5  
Affinity Capture-MS Homo sapiens
58 CRP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 P4HB 5034
Affinity Capture-MS Homo sapiens
60 ZZEF1 23140
Affinity Capture-MS Homo sapiens
61 FRAS1 80144
Affinity Capture-MS Homo sapiens
62 ZUFSP 221302
Affinity Capture-MS Homo sapiens
63 Pcsk5  
Affinity Capture-Western Mus musculus
64 B3GNT2 10678
Affinity Capture-MS Homo sapiens
65 CLPB 81570
Affinity Capture-MS Homo sapiens
66 COA7  
Affinity Capture-MS Homo sapiens
67 ST14 6768
Affinity Capture-MS Homo sapiens
68 POLE  
Affinity Capture-MS Homo sapiens
69 DGCR2 9993
Affinity Capture-MS Homo sapiens
70 TOR3A 64222
Affinity Capture-MS Homo sapiens
71 PASK  
Affinity Capture-MS Homo sapiens
72 MCM3AP  
Affinity Capture-MS Homo sapiens
73 TMEM59 9528
Affinity Capture-MS Homo sapiens
74 TIMP2 7077
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 MMP14 4323
Affinity Capture-MS Homo sapiens
76 FAM136A 84908
Affinity Capture-MS Homo sapiens
77 TMPRSS13  
Affinity Capture-MS Homo sapiens
78 PPM1A 5494
Affinity Capture-MS Homo sapiens
79 TMED10 10972
Affinity Capture-MS Homo sapiens
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