Gene description for NOTCH3
Gene name notch 3
Gene symbol NOTCH3
Other names/aliases CADASIL
CASIL
IMF2
Species Homo sapiens
 Database cross references - NOTCH3
ExoCarta ExoCarta_4854
Vesiclepedia VP_4854
Entrez Gene 4854
HGNC 7883
MIM 600276
UniProt Q9UM47  
 NOTCH3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NOTCH3
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IEA
    signaling receptor activity GO:0038023 IMP
    identical protein binding GO:0042802 IPI
    cadherin binding GO:0045296 HDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    Notch signaling pathway GO:0007219 IBA
    axon guidance GO:0007411 IBA
    neuroblast differentiation GO:0014016 IEA
    forebrain development GO:0030900 IEA
    negative regulation of neuron differentiation GO:0045665 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of smooth muscle cell proliferation GO:0048661 IEA
    neuron fate commitment GO:0048663 IEA
    artery morphogenesis GO:0048844 IEA
    glomerular capillary formation GO:0072104 IEA
    positive regulation of miRNA transcription GO:1902895 IMP
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    extracellular region GO:0005576 TAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IBA
    receptor complex GO:0043235 IBA
    receptor complex GO:0043235 IDA
 Experiment description of studies that identified NOTCH3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NOTCH3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 LRRIQ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RXFP1  
Affinity Capture-MS Homo sapiens
4 DLK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CNTD1  
Reconstituted Complex Homo sapiens
6 ADAM32  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 XKR8  
Reconstituted Complex Homo sapiens
8 LGALS1 3956
Affinity Capture-MS Homo sapiens
9 PPIA 5478
Reconstituted Complex Homo sapiens
10 IFNGR1 3459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 RET 5979
Reconstituted Complex Homo sapiens
12 RABGGTB 5876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PTCRA  
Affinity Capture-Western Homo sapiens
14 MFAP5  
Affinity Capture-MS Homo sapiens
15 CTXN1  
Affinity Capture-MS Homo sapiens
16 CRIP2 1397
Reconstituted Complex Homo sapiens
17 CPNE6 9362
Reconstituted Complex Homo sapiens
18 DNAJC10 54431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CBFA2T2  
Reconstituted Complex Homo sapiens
20 SLC20A1 6574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 JAG1 182
Reconstituted Complex Homo sapiens
22 ZNF74  
Affinity Capture-MS Homo sapiens
23 ST14 6768
Affinity Capture-MS Homo sapiens
24 IGFL3  
Affinity Capture-MS Homo sapiens
25 C8orf76  
Reconstituted Complex Homo sapiens
26 RMI2  
Reconstituted Complex Homo sapiens
27 CSNK2B 1460
Two-hybrid Homo sapiens
28 IL7 3574
Affinity Capture-MS Homo sapiens
29 GSK3B 2932
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
30 NOTCH2 4853
Affinity Capture-MS Homo sapiens
31 TPT1 7178
Reconstituted Complex Homo sapiens
32 NEDD9 4739
Reconstituted Complex Homo sapiens
33 MANSC1 54682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 FBLN1 2192
Two-hybrid Homo sapiens
35 IGSF5  
Affinity Capture-MS Homo sapiens
36 BTF3L4 91408
Reconstituted Complex Homo sapiens
37 TSPAN3 10099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CBL 867
Affinity Capture-Western Homo sapiens
39 HIF1AN 55662
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CDH1 999
Proximity Label-MS Homo sapiens
41 ZFP41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 C1QL1  
Reconstituted Complex Homo sapiens
43 NTRK1 4914
Affinity Capture-MS Homo sapiens
44 DTX2 113878
Proximity Label-MS Homo sapiens
45 GRIN1 2902
Affinity Capture-MS Homo sapiens
46 JAG2  
Reconstituted Complex Homo sapiens
47 BTNL3  
Affinity Capture-MS Homo sapiens
48 ANKRD44 91526
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 PPP1R12B 4660
Affinity Capture-MS Homo sapiens
50 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 MARCH1  
Affinity Capture-MS Homo sapiens
52 BAG2 9532
Reconstituted Complex Homo sapiens
53 KCNK18  
Affinity Capture-MS Homo sapiens
54 TNNT2  
Reconstituted Complex Homo sapiens
55 MAML1  
Reconstituted Complex Homo sapiens
56 WDR25  
Reconstituted Complex Homo sapiens
57 PRG2  
Affinity Capture-MS Homo sapiens
58 CUL3 8452
Affinity Capture-MS Homo sapiens
59 EGFL7 51162
Two-hybrid Homo sapiens
60 SLC39A1 27173
Reconstituted Complex Homo sapiens
61 STRAP 11171
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
62 SNX19  
Reconstituted Complex Homo sapiens
63 KCNE3  
Affinity Capture-MS Homo sapiens
64 MKRN2 23609
Affinity Capture-RNA Homo sapiens
65 MYC  
Reconstituted Complex Homo sapiens
66 FBXO2 26232
Affinity Capture-MS Homo sapiens
67 GGT6  
Reconstituted Complex Homo sapiens
68 DLL1  
Reconstituted Complex Homo sapiens
69 SORBS3 10174
Reconstituted Complex Homo sapiens
70 ZNF224  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 ZNF707  
Affinity Capture-MS Homo sapiens
72 RBPJ 3516
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
73 LINC00839  
Protein-RNA Homo sapiens
74 ADAM33 80332
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 ABCG8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 SCGB3A1 92304
Reconstituted Complex Homo sapiens
77 TMEM108  
Reconstituted Complex Homo sapiens
78 PSMA1 5682
Affinity Capture-Western Homo sapiens
79 WWP2 11060
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
80 NOTCH1 4851
Phenotypic Suppression Homo sapiens
Affinity Capture-MS Homo sapiens
81 NTN5  
Affinity Capture-MS Homo sapiens
82 NEU2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 SNW1 22938
Reconstituted Complex Homo sapiens
84 ZNF408  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 SPTLC2 9517
Reconstituted Complex Homo sapiens
86 MPPED2  
Reconstituted Complex Homo sapiens
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