Gene description for ASCC3
Gene name activating signal cointegrator 1 complex subunit 3
Gene symbol ASCC3
Other names/aliases ASC1p200
HELIC1
RNAH
Species Homo sapiens
 Database cross references - ASCC3
ExoCarta ExoCarta_10973
Vesiclepedia VP_10973
Entrez Gene 10973
HGNC 18697
MIM 614217
UniProt Q8N3C0  
 ASCC3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for ASCC3
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    ATP hydrolysis activity GO:0016887 IDA
    double-stranded DNA helicase activity GO:0036121 IEA
    3'-5' DNA helicase activity GO:0043138 IBA
    3'-5' DNA helicase activity GO:0043138 IDA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    DNA alkylation repair GO:0006307 IDA
    DNA alkylation repair GO:0006307 NAS
    chromatin remodeling GO:0006338 IEA
    DNA duplex unwinding GO:0032508 IDA
    DNA duplex unwinding GO:0032508 NAS
    ribosome disassembly GO:0032790 IDA
    ribosome disassembly GO:0032790 NAS
    rescue of stalled ribosome GO:0072344 IDA
    rescue of stalled ribosome GO:0072344 IMP
    chromatin looping GO:0140588 IEA
    ribosome-associated ubiquitin-dependent protein catabolic process GO:1990116 IMP
    ribosome-associated ubiquitin-dependent protein catabolic process GO:1990116 NAS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
    nuclear speck GO:0016607 IEA
    cytosolic ribosome GO:0022626 IDA
    activating signal cointegrator 1 complex GO:0099053 IDA
    DNA repair complex GO:1990391 IPI
 Experiment description of studies that identified ASCC3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ASCC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ISG15 9636
Affinity Capture-MS Homo sapiens
3 LGR4 55366
Affinity Capture-MS Homo sapiens
4 CIB2  
Affinity Capture-MS Homo sapiens
5 USP10 9100
Affinity Capture-MS Homo sapiens
6 HDHD1  
Affinity Capture-MS Homo sapiens
7 UBC 7316
Reconstituted Complex Homo sapiens
8 RIOK3 8780
Affinity Capture-MS Homo sapiens
9 CAND1 55832
Affinity Capture-MS Homo sapiens
10 RPS3 6188
Affinity Capture-MS Homo sapiens
11 HCCS 3052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 RPS20 6224
Proximity Label-MS Homo sapiens
13 IMPDH2 3615
Proximity Label-MS Homo sapiens
14 EIF3B 8662
Affinity Capture-MS Homo sapiens
15 FBXW7  
Affinity Capture-MS Homo sapiens
16 B4GALT2  
Affinity Capture-MS Homo sapiens
17 IQGAP1 8826
Affinity Capture-MS Homo sapiens
18 NPM1 4869
Affinity Capture-MS Homo sapiens
19 BRPF3  
Affinity Capture-MS Homo sapiens
20 GSPT1 2935
Affinity Capture-MS Homo sapiens
21 HSP90B1 7184
Cross-Linking-MS (XL-MS) Homo sapiens
22 PVALB  
Affinity Capture-MS Homo sapiens
23 DGCR2 9993
Affinity Capture-MS Homo sapiens
24 SRP68 6730
Affinity Capture-MS Homo sapiens
25 DNAJC7 7266
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 ATG16L1 55054
Affinity Capture-MS Homo sapiens
27 DDX6 1656
Affinity Capture-MS Homo sapiens
28 RPS11 6205
Affinity Capture-MS Homo sapiens
29 RBM8A 9939
Affinity Capture-MS Homo sapiens
30 Rrp1  
Two-hybrid Mus musculus
31 FAM96A  
Affinity Capture-MS Homo sapiens
32 MIB1 57534
Proximity Label-MS Homo sapiens
33 PLBD1 79887
Affinity Capture-MS Homo sapiens
34 CD74 972
Affinity Capture-MS Homo sapiens
35 KLHL34  
Affinity Capture-MS Homo sapiens
36 RBM4B  
Affinity Capture-MS Homo sapiens
37 PURG  
Affinity Capture-MS Homo sapiens
38 Srp72  
Affinity Capture-MS Mus musculus
39 RPS10 6204
Affinity Capture-MS Homo sapiens
40 NT5C 30833
Affinity Capture-MS Homo sapiens
41 NTRK1 4914
Affinity Capture-MS Homo sapiens
42 ENTPD1 953
Affinity Capture-MS Homo sapiens
43 ALKBH3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 THBS3 7059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ASCC2 84164
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
46 TTC9C 283237
Affinity Capture-MS Homo sapiens
47 ENO1 2023
Affinity Capture-RNA Homo sapiens
48 HECTD1 25831
Affinity Capture-MS Homo sapiens
49 ARPC5 10092
Affinity Capture-MS Homo sapiens
50 HSPB8 26353
Affinity Capture-MS Homo sapiens
51 UBXN6 80700
Affinity Capture-MS Homo sapiens
52 LOC388882  
Affinity Capture-MS Homo sapiens
53 ZNF252P-AS1  
Affinity Capture-MS Homo sapiens
54 EIF3G 8666
Affinity Capture-MS Homo sapiens
55 SLX4  
Affinity Capture-MS Homo sapiens
56 ABT1 29777
Affinity Capture-MS Homo sapiens
57 RPS2 6187
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 BAG2 9532
Affinity Capture-MS Homo sapiens
59 ASCC1 51008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
60 SIRT7  
Affinity Capture-MS Homo sapiens
61 NPM3 10360
Affinity Capture-MS Homo sapiens
62 CUL3 8452
Affinity Capture-MS Homo sapiens
63 Rrbp1  
Affinity Capture-MS Mus musculus
64 EGFR 1956
Negative Genetic Homo sapiens
65 METTL21B  
Affinity Capture-MS Homo sapiens
66 RBBP4 5928
Affinity Capture-MS Homo sapiens
67 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
68 NXF1 10482
Affinity Capture-RNA Homo sapiens
69 DGKZ  
Affinity Capture-MS Homo sapiens
70 PSPC1 55269
Affinity Capture-MS Homo sapiens
71 HECW2  
Affinity Capture-MS Homo sapiens
72 RPS13 6207
Affinity Capture-MS Homo sapiens
73 PABPC5 140886
Affinity Capture-MS Homo sapiens
74 GOLGA1  
Proximity Label-MS Homo sapiens
75 Rpl35 66489
Affinity Capture-MS Mus musculus
76 NOG 9241
Affinity Capture-MS Homo sapiens
77 C9orf72  
Affinity Capture-MS Homo sapiens
78 ZNF263  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 CCDC8  
Affinity Capture-MS Homo sapiens
80 RPS16 6217
Affinity Capture-MS Homo sapiens
81 EIF3F 8665
Affinity Capture-MS Homo sapiens
82 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
83 SIRT6  
Affinity Capture-MS Homo sapiens
84 SKIL  
Two-hybrid Homo sapiens
85 UBXN4 23190
Cross-Linking-MS (XL-MS) Homo sapiens
86 PDCD6IP 10015
Cross-Linking-MS (XL-MS) Homo sapiens
87 TRIM52  
Affinity Capture-MS Homo sapiens
88 SQSTM1 8878
Proximity Label-MS Homo sapiens
89 G3BP1 10146
Affinity Capture-MS Homo sapiens
90 CAPRIN1 4076
Affinity Capture-MS Homo sapiens
91 G3BP2 9908
Affinity Capture-MS Homo sapiens
92 OTUD6B 51633
Affinity Capture-MS Homo sapiens
93 CDK1 983
Affinity Capture-MS Homo sapiens
94 DNAJA2 10294
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 PPP4R1L  
Affinity Capture-MS Homo sapiens
96 MOV10 4343
Affinity Capture-RNA Homo sapiens
97 INF2 64423
Affinity Capture-MS Homo sapiens
98 TRIP4 9325
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 RPS6 6194
Affinity Capture-MS Homo sapiens
100 BEX1  
Affinity Capture-MS Homo sapiens
101 BRD1 23774
Affinity Capture-MS Homo sapiens
102 HSD17B7P2  
Affinity Capture-MS Homo sapiens
103 PTGES3 10728
Affinity Capture-MS Homo sapiens
104 GNB2L1 10399
Affinity Capture-MS Homo sapiens
105 SRP9 6726
Affinity Capture-MS Homo sapiens
106 RPA3 6119
Proximity Label-MS Homo sapiens
107 NOTCH1 4851
Affinity Capture-MS Homo sapiens
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