Gene description for ANP32E
Gene name acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
Gene symbol ANP32E
Other names/aliases LANP-L
LANPL
Species Homo sapiens
 Database cross references - ANP32E
ExoCarta ExoCarta_81611
Vesiclepedia VP_81611
Entrez Gene 81611
HGNC 16673
MIM 609611
UniProt Q9BTT0  
 ANP32E identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for ANP32E
Molecular Function
    protein binding GO:0005515 IPI
    phosphatase inhibitor activity GO:0019212 IBA
    phosphatase inhibitor activity GO:0019212 ISS
    histone binding GO:0042393 IBA
    histone binding GO:0042393 IDA
    protein folding chaperone GO:0044183 EXP
    histone chaperone activity GO:0140713 IDA
Biological Process
    chromatin organization GO:0006325 IEA
    protein folding GO:0006457 IEA
    regulation of apoptotic process GO:0042981 IBA
Subcellular Localization
    Swr1 complex GO:0000812 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISS
    synaptic vesicle membrane GO:0030672 IEA
    cytoplasmic vesicle GO:0031410 ISS
    postsynaptic density membrane GO:0098839 IEA
    postsynaptic endosome membrane GO:0098895 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified ANP32E in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
12
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ANP32E
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD3EAP  
Proximity Label-MS Homo sapiens
2 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
3 CLPP 8192
Proximity Label-MS Homo sapiens
4 VPS35 55737
Co-fractionation Homo sapiens
5 BRD7  
Affinity Capture-MS Homo sapiens
6 EIF3G 8666
Co-fractionation Homo sapiens
7 HIST1H2BE 8344
Affinity Capture-MS Homo sapiens
8 HIST1H2BO 8348
Affinity Capture-MS Homo sapiens
9 RNASEH2B  
Co-fractionation Homo sapiens
10 SERBP1 26135
Affinity Capture-MS Homo sapiens
11 DNAJB6 10049
Proximity Label-MS Homo sapiens
12 SARS 6301
Co-fractionation Homo sapiens
13 ANP32A 8125
Co-fractionation Homo sapiens
14 RPS24 6229
Proximity Label-MS Homo sapiens
15 RPS2 6187
Co-fractionation Homo sapiens
16 KPNA1 3836
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 UBA5 79876
Affinity Capture-MS Homo sapiens
18 TPGS1  
Affinity Capture-MS Homo sapiens
19 UNK  
Affinity Capture-RNA Homo sapiens
20 ATP6V1C1 528
Co-fractionation Homo sapiens
21 PARD3 56288
Affinity Capture-MS Homo sapiens
22 RPL27 6155
Cross-Linking-MS (XL-MS) Homo sapiens
23 HIST1H2BG 8339
Affinity Capture-MS Homo sapiens
24 SRP9 6726
Affinity Capture-MS Homo sapiens
25 ACTC1 70
Proximity Label-MS Homo sapiens
26 NOP56 10528
Proximity Label-MS Homo sapiens
27 KPNA6 23633
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 FOXC1  
Affinity Capture-MS Homo sapiens
29 KPNA5 3841
Affinity Capture-MS Homo sapiens
30 AHCYL2 23382
Co-fractionation Homo sapiens
31 UBC 7316
Reconstituted Complex Homo sapiens
32 HIST3H2BB 128312
Affinity Capture-MS Homo sapiens
33 PTPN2 5771
Affinity Capture-MS Homo sapiens
34 MEPCE 56257
Affinity Capture-MS Homo sapiens
35 WHSC1 7468
Proximity Label-MS Homo sapiens
36 HIST1H2AG 8969
Affinity Capture-MS Homo sapiens
37 SNX6 58533
Co-fractionation Homo sapiens
38 NIFK 84365
Proximity Label-MS Homo sapiens
39 PBRM1 55193
Affinity Capture-MS Homo sapiens
40 PAPPA 5069
Co-fractionation Homo sapiens
41 ESR1  
Affinity Capture-MS Homo sapiens
42 CBX3 11335
Proximity Label-MS Homo sapiens
43 DMAP1 55929
Co-fractionation Homo sapiens
44 DDX23 9416
Proximity Label-MS Homo sapiens
45 HIST1H2BD 3017
Affinity Capture-MS Homo sapiens
46 E2F4  
Affinity Capture-MS Homo sapiens
47 RDX 5962
Co-fractionation Homo sapiens
48 FOS 2353
Reconstituted Complex Homo sapiens
49 PRC1 9055
Affinity Capture-MS Homo sapiens
50 HIST1H2BA 255626
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 KIF23 9493
Affinity Capture-MS Homo sapiens
52 DNAJC9 23234
Proximity Label-MS Homo sapiens
53 TPI1 7167
Co-fractionation Homo sapiens
54 RPL31 6160
Proximity Label-MS Homo sapiens
55 CCT2 10576
Cross-Linking-MS (XL-MS) Homo sapiens
56 HIST2H2AB 317772
Affinity Capture-MS Homo sapiens
57 SMAD2 4087
Reconstituted Complex Homo sapiens
58 SNX2 6643
Co-fractionation Homo sapiens
59 PARP1 142
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 POLR1D 51082
Affinity Capture-MS Homo sapiens
61 FYTTD1  
Affinity Capture-MS Homo sapiens
62 POLR1E  
Affinity Capture-MS Homo sapiens
63 H2AFZ 3015
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 VPS72  
Co-fractionation Homo sapiens
65 PMAIP1  
Affinity Capture-MS Homo sapiens
66 UCHL5 51377
Reconstituted Complex Homo sapiens
67 HIST1H2BI 8346
Affinity Capture-MS Homo sapiens
68 CEBPA  
Proximity Label-MS Homo sapiens
69 HIST1H2AB 8335
Affinity Capture-MS Homo sapiens
70 AHCYL1 10768
Co-fractionation Homo sapiens
71 ATG7 10533
Co-fractionation Homo sapiens
72 MGMT 4255
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ANP32E is involved
No pathways found





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