Gene description for MX1
Gene name MX dynamin-like GTPase 1
Gene symbol MX1
Other names/aliases IFI-78K
IFI78
MX
MxA
Species Homo sapiens
 Database cross references - MX1
ExoCarta ExoCarta_4599
Entrez Gene 4599
HGNC 7532
MIM 147150
UniProt P20591  
 MX1 identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Thymus 23844026    
 Gene ontology annotations for MX1
Molecular Function
    GTP binding GO:0005525 IEA
    protein binding GO:0005515 IPI
    GTPase activity GO:0003924 IEA
Biological Process
    cytokine-mediated signaling pathway GO:0019221 TAS
    signal transduction GO:0007165 TAS
    metabolic process GO:0008152 IEA
    response to virus GO:0009615 IMP
    response to type I interferon GO:0034340 TAS
    defense response GO:0006952 TAS
    negative regulation of viral genome replication GO:0045071 IDA
    type I interferon signaling pathway GO:0060337 TAS
    innate immune response GO:0045087 TAS
    defense response to virus GO:0051607 IEA
    apoptotic process GO:0006915 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    endoplasmic reticulum membrane GO:0005789 IEA
    nuclear membrane GO:0031965 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified MX1 in exosomes
1
Experiment ID 76
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|GAPDH|HSP90
EV Cytosolic markers
✔
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 140
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
LAMP2|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
3
Experiment ID 141
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
LAMP2|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BLM 641
Two-hybrid Homo sapiens
2 FANCA  
Two-hybrid Homo sapiens
3 TUBB2A 7280
Affinity Capture-MS Homo sapiens
4 DAXX  
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 MX1 4599
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MX1 is involved
PathwayEvidenceSource
Interferon alpha/beta signaling TAS Reactome
ISG15 antiviral mechanism TAS Reactome





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