Gene description for GABARAP
Gene name GABA(A) receptor-associated protein
Gene symbol GABARAP
Other names/aliases ATG8A
GABARAP-a
MM46
Species Homo sapiens
 Database cross references - GABARAP
ExoCarta ExoCarta_11337
Vesiclepedia VP_11337
Entrez Gene 11337
HGNC 4067
MIM 605125
UniProt O95166  
 GABARAP identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GABARAP
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IEA
    phosphatidylethanolamine binding GO:0008429 IBA
    phosphatidylethanolamine binding GO:0008429 IDA
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin protein ligase binding GO:0031625 IPI
    beta-tubulin binding GO:0048487 IDA
    GABA receptor binding GO:0050811 IBA
    GABA receptor binding GO:0050811 IPI
Biological Process
    autophagosome assembly GO:0000045 IBA
    microtubule cytoskeleton organization GO:0000226 IEA
    mitophagy GO:0000423 IBA
    protein targeting GO:0006605 TAS
    cellular response to nitrogen starvation GO:0006995 IBA
    chemical synaptic transmission GO:0007268 TAS
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IDA
    protein transport GO:0015031 IEA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IMP
    regulation of Rac protein signal transduction GO:0035020 IMP
    autophagosome maturation GO:0097352 IBA
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IEA
    positive regulation of protein K48-linked ubiquitination GO:1902524 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    autophagosome membrane GO:0000421 IBA
    autophagosome membrane GO:0000421 IDA
    autophagosome membrane GO:0000421 TAS
    lysosome GO:0005764 IEA
    autophagosome GO:0005776 IDA
    smooth endoplasmic reticulum GO:0005790 IEA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    microtubule associated complex GO:0005875 IEA
    plasma membrane GO:0005886 IEA
    axoneme GO:0005930 ISS
    actin cytoskeleton GO:0015629 IEA
    cytoplasmic vesicle GO:0031410 IEA
    sperm midpiece GO:0097225 IEA
    GABA-ergic synapse GO:0098982 IEA
 Experiment description of studies that identified GABARAP in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GABARAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GABARAPL1 23710
Affinity Capture-MS Homo sapiens
2 MAP1LC3C  
Affinity Capture-MS Homo sapiens
3 TCEB1 6921
Co-fractionation Homo sapiens
4 CHRNA4  
Affinity Capture-MS Homo sapiens
5 GJA1 2697
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
6 SRPK2 6733
Biochemical Activity Homo sapiens
7 MTX1 4580
Reconstituted Complex Homo sapiens
8 MTMR14 64419
Affinity Capture-MS Homo sapiens
9 CALCOCO1 57658
Affinity Capture-Western Homo sapiens
10 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
11 BNIP3L  
Affinity Capture-Western Homo sapiens
12 FNIP1  
Affinity Capture-MS Homo sapiens
13 ATG4C  
Reconstituted Complex Homo sapiens
14 MEFV  
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
15 ATG13 9776
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 MAP1LC3B 81631
Affinity Capture-MS Homo sapiens
17 GABRG2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
18 ATXN7L1 222255
Affinity Capture-MS Homo sapiens
19 CALCOCO2  
Affinity Capture-Western Homo sapiens
20 CCPG1 9236
Affinity Capture-MS Homo sapiens
21 TBC1D15 64786
Affinity Capture-MS Homo sapiens
22 NCOA7  
Affinity Capture-MS Homo sapiens
23 KATNB1  
Co-fractionation Homo sapiens
24 ATG7 10533
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
25 ATG4D  
Reconstituted Complex Homo sapiens
26 WDFY3 23001
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 RAD50 10111
Co-fractionation Homo sapiens
28 NEDD4 4734
Affinity Capture-MS Homo sapiens
29 TFRC 7037
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
30 DVL2 1856
Reconstituted Complex Homo sapiens
31 KDR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
32 CCT2 10576
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
33 TP53INP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Two-hybrid Homo sapiens
34 RWDD2A  
Affinity Capture-MS Homo sapiens
35 TCP1 6950
Affinity Capture-Western Homo sapiens
36 ATG4A  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
37 MIB1 57534
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
38 SAMM50 25813
Reconstituted Complex Homo sapiens
39 RB1CC1 9821
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
40 TGDS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 AMFR 267
Affinity Capture-MS Homo sapiens
42 NBR1 4077
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
43 TSPAN3 10099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 OPTN 10133
Reconstituted Complex Homo sapiens
45 ULK2  
Protein-peptide Homo sapiens
46 CARM1 10498
Co-fractionation Homo sapiens
47 GABARAP 11337
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
48 TRIM21 6737
Reconstituted Complex Homo sapiens
49 ULK1  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
50 FNBP1 23048
Affinity Capture-MS Homo sapiens
51 HDAC5 10014
Two-hybrid Homo sapiens
52 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
53 SRPK1 6732
Biochemical Activity Homo sapiens
54 TSR2  
Two-hybrid Homo sapiens
55 FOXN4  
Affinity Capture-MS Homo sapiens
56 STK3 6788
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
57 RAB6A 5870
Cross-Linking-MS (XL-MS) Homo sapiens
58 TECPR2  
Affinity Capture-MS Homo sapiens
59 IPO5 3843
Affinity Capture-MS Homo sapiens
60 TAX1BP1 8887
Reconstituted Complex Homo sapiens
61 AMBRA1  
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
62 CCT6A 908
Affinity Capture-Western Homo sapiens
63 ATG12  
Affinity Capture-MS Homo sapiens
64 PRKCZ 5590
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
65 SNX18 112574
Reconstituted Complex Homo sapiens
66 PI4K2A 55361
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 DYX1C1  
Affinity Capture-MS Homo sapiens
68 MUL1  
Affinity Capture-Western Homo sapiens
69 RPA3 6119
Proximity Label-MS Homo sapiens
70 STBD1 8987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 PCM1 5108
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
72 STK4 6789
Affinity Capture-Western Homo sapiens
73 MAPK15  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
74 TBC1D25  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
75 GBAS 2631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 ANKFY1 51479
Affinity Capture-MS Homo sapiens
77 NUFIP2 57532
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
78 KEAP1 9817
Affinity Capture-MS Homo sapiens
79 GABARAPL2 11345
Affinity Capture-MS Homo sapiens
80 TTBK1  
Affinity Capture-MS Homo sapiens
81 TUBB3 10381
Two-hybrid Homo sapiens
82 SQSTM1 8878
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
83 G3BP1 10146
Reconstituted Complex Homo sapiens
84 ATG101  
Two-hybrid Homo sapiens
85 FKBP4 2288
Affinity Capture-MS Homo sapiens
86 MLH1 4292
Two-hybrid Homo sapiens
87 FNIP2  
Affinity Capture-Western Homo sapiens
88 DDX58 23586
Affinity Capture-RNA Homo sapiens
89 FLCN 201163
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
90 SEC62 7095
Affinity Capture-MS Homo sapiens
91 ATG4B 23192
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
92 NEK9 91754
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
93 ATG3 64422
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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