Gene description for GCA
Gene name grancalcin, EF-hand calcium binding protein
Gene symbol GCA
Other names/aliases GCL
Species Homo sapiens
 Database cross references - GCA
ExoCarta ExoCarta_25801
Vesiclepedia VP_25801
Entrez Gene 25801
HGNC 15990
MIM 607030
UniProt P28676  
 GCA identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for GCA
Molecular Function
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IPI
    protein heterodimerization activity GO:0046982 IPI
Biological Process
    membrane fusion GO:0061025 TAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    azurophil granule lumen GO:0035578 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified GCA in sEVs
1
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for GCA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MTSS1L 92154
Affinity Capture-MS Homo sapiens
2 MAGEA8  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 ATXN1 6310
Two-hybrid Homo sapiens
4 CIR1  
Affinity Capture-MS Homo sapiens
5 BPIFA1 51297
Two-hybrid Homo sapiens
6 GCA 25801
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
7 DUS4L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TRAF2 7186
Two-hybrid Homo sapiens
9 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PRNP 5621
Affinity Capture-MS Homo sapiens
11 TMEM255A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 FBXL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 C11orf49  
Two-hybrid Homo sapiens
14 STMN4  
Affinity Capture-MS Homo sapiens
15 NEU4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CDC37 11140
Affinity Capture-MS Homo sapiens
17 ICMT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 FGB 2244
Affinity Capture-MS Homo sapiens
19 PSMB4 5692
Two-hybrid Homo sapiens
20 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
21 RBFOX1 54715
Two-hybrid Homo sapiens
22 DDX58 23586
Affinity Capture-RNA Homo sapiens
23 RHBDD1 84236
Affinity Capture-MS Homo sapiens
24 GOLGA8A 23015
Affinity Capture-MS Homo sapiens
25 CEACAM16  
Affinity Capture-MS Homo sapiens
26 SYPL2  
Affinity Capture-MS Homo sapiens
27 ABHD11 83451
Two-hybrid Homo sapiens
28 MYH9 4627
Affinity Capture-MS Homo sapiens
29 SAR1B 51128
Affinity Capture-MS Homo sapiens
30 C10orf55  
Two-hybrid Homo sapiens
31 LCP1 3936
Affinity Capture-Western Homo sapiens
32 AIP 9049
Co-fractionation Homo sapiens
33 RAB5C 5878
Affinity Capture-MS Homo sapiens
34 PARVA 55742
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CALCOCO2  
Two-hybrid Homo sapiens
36 MFAP4 4239
Affinity Capture-MS Homo sapiens
37 EPB41L5 57669
Affinity Capture-MS Homo sapiens
38 KLK10 5655
Affinity Capture-MS Homo sapiens
39 CYP3A43  
Affinity Capture-MS Homo sapiens
40 GNG8  
Affinity Capture-MS Homo sapiens
41 SRI 6717
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
42 TCEB3B  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GCA is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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