|
 Gene ontology annotations for CD9 |
|
|
| Experiment description of studies that identified CD9 in sEVs |
| 1 |
| Experiment ID |
58 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
HSP70|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
17700640
|
| Organism |
Homo sapiens |
| Experiment description |
CD24 is a marker of exosomes secreted into urine and amniotic fluid. |
| Authors |
"Keller S, Rupp C, Stoeck A, Runz S, Fogel M, Lugert S, Hager HD, Abdel-Bakky MS, Gutwein P, Altevogt P" |
| Journal name |
KI
|
| Publication year |
2007 |
| Sample |
Amniotic fluid |
| Sample name |
Amniotic fluid |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.08-1.14 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 2 |
| Experiment ID |
110 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
HSP70|CD81|CD9|HLA-DR|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
21601258
|
| Organism |
Homo sapiens |
| Experiment description |
Loss of EpCAM expression in breast cancer derived serum exosomes: Role of proteolytic cleavage. |
| Authors |
"Rupp AK, Rupp C, Keller S, Brase JC, Ehehalt R, Fogel M, Moldenhauer G, Marme F, Sultmann H, Altevogt P." |
| Journal name |
GO
|
| Publication year |
2011 |
| Sample |
Ascites |
| Sample name |
Ascites - Liver cirrhosis |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.04-1.10 g/mL
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Immunoelctron microscopy |
|
|
| 3 |
| Experiment ID |
116 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21505438
|
| Organism |
Homo sapiens |
| Experiment description |
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. |
| Authors |
"Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÃÂ, Bernad A, Sánchez-Madrid F." |
| Journal name |
NATCOM
|
| Publication year |
2011 |
| Sample |
B cells |
| Sample name |
Raji |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Mass spectrometry
Microarray |
|
|
| 4 |
| Experiment ID |
76 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
|
Enriched markers |
|
✔
HSP90B1
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
Western blotting
FACS
|
| PubMed ID |
20224111
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomics analysis of bladder cancer exosomes. |
| Authors |
"Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A." |
| Journal name |
MCP
|
| Publication year |
2010 |
| Sample |
Bladder cancer cells |
| Sample name |
HT1376 |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS |
|
|
| 5 |
| Experiment ID |
42 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD86|TFRC|LAMP2|ITGA2B|CD63|CD9|MHCI|CD81
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
15908444
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal-like vesicles are present in human blood plasma. |
| Authors |
"Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C" |
| Journal name |
INTIMM
|
| Publication year |
2005 |
| Sample |
Bone marrow cells |
| Sample name |
PBMC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 6 |
| Experiment ID |
489 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 7 |
| Experiment ID |
490 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 8 |
| Experiment ID |
491 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 8 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 9 |
| Experiment ID |
492 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
Enriched markers |
|
✔
Canx
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 9 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 10 |
| Experiment ID |
397 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✔
GOLGA2|CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunofluorescence
Flow cytomtery |
|
|
| 11 |
| Experiment ID |
397 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✔
GOLGA2|CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immunofluorescence
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunofluorescence
Flow cytomtery |
|
|
| 12 |
| Experiment ID |
397 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✔
GOLGA2|CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunofluorescence
Flow cytomtery |
|
|
| 13 |
| Experiment ID |
398 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✔
GOLGA2|CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunofluorescence
Flow cytomtery |
|
|
| 14 |
| Experiment ID |
398 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✔
GOLGA2|CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immunofluorescence
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunofluorescence
Flow cytomtery |
|
|
| 15 |
| Experiment ID |
398 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✔
GOLGA2|CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immunofluorescence
Flow cytomtery |
|
|
| 16 |
| Experiment ID |
412 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX|ACTB
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 17 |
| Experiment ID |
412 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX|ACTB
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 18 |
| Experiment ID |
414 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 19 |
| Experiment ID |
414 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 20 |
| Experiment ID |
426 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 21 |
| Experiment ID |
426 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 22 |
| Experiment ID |
427 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 23 |
| Experiment ID |
522 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
FLOT1|CD9
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34112803
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution |
| Authors |
"Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 24 |
| Experiment ID |
523 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
FLOT1|CD9
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34112803
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution |
| Authors |
"Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 25 |
| Experiment ID |
527 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34112803
|
| Organism |
Mus musculus |
| Experiment description |
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution |
| Authors |
"Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
EO771 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 26 |
| Experiment ID |
528 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34112803
|
| Organism |
Mus musculus |
| Experiment description |
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution |
| Authors |
"Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
EO771 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 27 |
| Experiment ID |
529 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34112803
|
| Organism |
Mus musculus |
| Experiment description |
Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution |
| Authors |
"Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
EO771 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 28 |
| Experiment ID |
920 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 29 |
| Experiment ID |
921 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 30 |
| Experiment ID |
924 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|SDCBP|CD81|RAB35|LAMP2|FLOT1|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 31 |
| Experiment ID |
925 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 32 |
| Experiment ID |
926 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|FLOT1|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 33 |
| Experiment ID |
927 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|SDCBP|CD81|RAB35|LAMP2|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 34 |
| Experiment ID |
930 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|FLOT1|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 35 |
| Experiment ID |
931 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 36 |
| Experiment ID |
932 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|FLOT1|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 37 |
| Experiment ID |
933 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|FLOT1|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Breast cancer cells |
| Sample name |
MCF7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 38 |
| Experiment ID |
1331 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|ICAM1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34796683
|
| Organism |
Homo sapiens |
| Experiment description |
High TSPAN8 expression in epithelial cancer cell-derived small extracellular vesicles promote confined diffusion and pronounced uptake |
| Authors |
"Wang T, Wang X, Wang H, Li L, Zhang C, Xiang R, Tan X, Li Z, Jiang C, Zheng L, Xiao L, Yue S." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
T-47D |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 39 |
| Experiment ID |
1332 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|ICAM1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34796683
|
| Organism |
Homo sapiens |
| Experiment description |
High TSPAN8 expression in epithelial cancer cell-derived small extracellular vesicles promote confined diffusion and pronounced uptake |
| Authors |
"Wang T, Wang X, Wang H, Li L, Zhang C, Xiang R, Tan X, Li Z, Jiang C, Zheng L, Xiao L, Yue S." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
T-47D |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 40 |
| Experiment ID |
1333 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|EPCAM|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35918900
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) |
| Authors |
"Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectometry
Western blotting |
|
|
| 41 |
| Experiment ID |
1333 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|EPCAM|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35918900
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) |
| Authors |
"Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectometry
Western blotting |
|
|
| 42 |
| Experiment ID |
1334 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✔
ACTB
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35918900
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) |
| Authors |
"Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
LM2 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectometry |
|
|
| 43 |
| Experiment ID |
1335 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
EPCAM|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35918900
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) |
| Authors |
"Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectometry |
|
|
| 44 |
| Experiment ID |
1335 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
EPCAM|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35918900
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) |
| Authors |
"Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectometry |
|
|
| 45 |
| Experiment ID |
1336 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✔
ACTB
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35918900
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) |
| Authors |
"Bauzá-Martinez J, Armony G, Pronker MF, Wu W. " |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Breast cancer cells |
| Sample name |
LM2 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectometry |
|
|
| 46 |
| Experiment ID |
124 |
| MISEV standards |
|
✔
IEM|EM
|
Biophysical techniques |
|
✔
HSC70|CD63|CD9|CD81
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
21235781
|
| Organism |
Homo sapiens |
| Experiment description |
"Human saliva, plasma and breast milk exosomes contain RNA: uptake by macrophages." |
| Authors |
"Lasser C, Alikhani VS, Ekstrom K, Eldh M, Paredes PT, Bossios A, Sjostrand M, Gabrielsson S, Lotvall J, Valadi H." |
| Journal name |
JTM
|
| Publication year |
2011 |
| Sample |
Breast milk |
| Sample name |
Breast milk - Normal |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
FACS
Immunoelectron Microscopy
Western blotting |
|
|
| 47 |
| Experiment ID |
388 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33767144
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative characterization of extracellular vesicle uptake and content delivery within mammalian cells |
| Authors |
"Bonsergent E, Grisard E, Buchrieser J, Schwartz O, Théry C, Lavieu G." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 48 |
| Experiment ID |
789 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35649370
|
| Organism |
Homo sapiens |
| Experiment description |
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells |
| Authors |
"Matsui T, Sakamaki Y, Nakashima S, Fukuda M." |
| Journal name |
Cell Rep
|
| Publication year |
2022 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa |
| Isolation/purification methods |
Differential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 49 |
| Experiment ID |
790 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35649370
|
| Organism |
Homo sapiens |
| Experiment description |
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells |
| Authors |
"Matsui T, Sakamaki Y, Nakashima S, Fukuda M." |
| Journal name |
Cell Rep
|
| Publication year |
2022 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa |
| Isolation/purification methods |
Differential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 50 |
| Experiment ID |
791 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35649370
|
| Organism |
Homo sapiens |
| Experiment description |
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells |
| Authors |
"Matsui T, Sakamaki Y, Nakashima S, Fukuda M." |
| Journal name |
Cell Rep
|
| Publication year |
2022 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa |
| Isolation/purification methods |
Differential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 51 |
| Experiment ID |
792 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
HSP90AA1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35649370
|
| Organism |
Homo sapiens |
| Experiment description |
Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells |
| Authors |
"Matsui T, Sakamaki Y, Nakashima S, Fukuda M." |
| Journal name |
Cell Rep
|
| Publication year |
2022 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa |
| Isolation/purification methods |
Differential centrifugation
Filtration
Immunoaffinity capture
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 52 |
| Experiment ID |
1325 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|LAMP1
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
immuno-EM
|
| PubMed ID |
34282141
|
| Organism |
Homo sapiens |
| Experiment description |
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 |
| Authors |
"Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa - 200K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Immuno-EM |
|
|
| 53 |
| Experiment ID |
1326 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34282141
|
| Organism |
Homo sapiens |
| Experiment description |
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 |
| Authors |
"Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa - 200K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 54 |
| Experiment ID |
1327 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34282141
|
| Organism |
Homo sapiens |
| Experiment description |
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 |
| Authors |
"Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa - 200K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 55 |
| Experiment ID |
1328 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|SDCBP|CD81|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34282141
|
| Organism |
Homo sapiens |
| Experiment description |
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 |
| Authors |
"Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa - 200K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 56 |
| Experiment ID |
1329 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|SDCBP|LAMP1|CD81|CD9
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34282141
|
| Organism |
Homo sapiens |
| Experiment description |
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 |
| Authors |
"Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa - 200K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 57 |
| Experiment ID |
1330 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD63|SDCBP|CD81|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34282141
|
| Organism |
Homo sapiens |
| Experiment description |
Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9 |
| Authors |
"Mathieu M, Névo N, Jouve M, Valenzuela JI, Maurin M, Verweij FJ, Palmulli R, Lankar D, Dingli F, Loew D, Rubinstein E, Boncompain G, Perez F, Théry C." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Cervical cancer cells |
| Sample name |
HeLa - 200K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 58 |
| Experiment ID |
494 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Osteoarthritic cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 59 |
| Experiment ID |
494 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Osteoarthritic cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 60 |
| Experiment ID |
495 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Healthy cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 61 |
| Experiment ID |
496 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Healthy cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 62 |
| Experiment ID |
496 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Healthy cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 63 |
| Experiment ID |
497 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 64 |
| Experiment ID |
497 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 65 |
| Experiment ID |
498 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 66 |
| Experiment ID |
498 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
"Varela-EirÃn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 67 |
| Experiment ID |
1213 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
FLOT1|CD9|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colon cancer cells |
| Sample name |
DiFi-derived EGFR/CD81-positive sEVs - dim (FAVS) |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 68 |
| Experiment ID |
1214 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
EPCAM|FLOT1|CD9|SDCBP
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colon cancer cells |
| Sample name |
DiFi-derived EGFR/CD81-positive sEVs - bright (FAVS) |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 69 |
| Experiment ID |
20 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP90|CD63|CD81|LAMP1
|
Enriched markers |
|
✔
GOLGA2|cytochrome c
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
17956143
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of microvesicles derived from human colorectal cancer cells. |
| Authors |
"Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS" |
| Journal name |
JPR
|
| Publication year |
2007 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT29 |
| Isolation/purification methods |
Differential centrifugation
Sucrose density gradient
Diafiltration |
| Flotation density |
1.16 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LTQ]
Western blotting |
|
|
| 70 |
| Experiment ID |
21 |
| MISEV standards |
|
✔
EM|IEM
|
Biophysical techniques |
|
✔
Alix|TSG101|HSP70|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
|
| PubMed ID |
19837982
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. |
| Authors |
"Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" |
| Journal name |
MCP
|
| Publication year |
2009 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1215 |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.10-1.12 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [Orbitrap]
Western blotting |
|
|
| 71 |
| Experiment ID |
201 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Alix|TSG101|HSP70|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
22740476
|
| Organism |
Homo sapiens |
| Experiment description |
Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4. |
| Authors |
"Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ." |
| Journal name |
Electrophoresis
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
SW480 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 72 |
| Experiment ID |
207 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
|
✔
VDAC
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
DKO-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 73 |
| Experiment ID |
208 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
|
✔
VDAC
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
Dks-8 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 74 |
| Experiment ID |
209 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|HSP70|FLOT1
|
Enriched markers |
|
✔
VDAC
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23161513
|
| Organism |
Homo sapiens |
| Experiment description |
Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. |
| Authors |
"Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." |
| Journal name |
Mol Cell Proteomics
|
| Publication year |
2012 |
| Sample |
Colorectal cancer cells |
| Sample name |
DLD-1 |
| Isolation/purification methods |
Differential centrifugation
Filtration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 75 |
| Experiment ID |
223 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
25330373
|
| Organism |
Homo sapiens |
| Experiment description |
Deep sequencing of RNA from three different extracellular vesicle (EV) subtypes released from the human LIM1863 colon cancer cell line uncovers distinct miRNA-enrichment signatures |
| Authors |
"Ji H, Chen M, Greening DW, He W, Rai A, Zhang W, Simpson RJ" |
| Journal name |
PLoS One
|
| Publication year |
2014 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Immunobeads (EpCAM)
Immunobeads (A33) |
| Flotation density |
-
|
| Molecules identified in the study |
miRNA
Protein |
| Methods used in the study |
RT-PCR
Western blotting |
|
|
| 76 |
| Experiment ID |
282 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Ultracentrifugation - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 77 |
| Experiment ID |
283 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 78 |
| Experiment ID |
285 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 79 |
| Experiment ID |
286 |
| MISEV standards |
|
✔
CEM
|
Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
DLS
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ." |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 80 |
| Experiment ID |
326 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32438511
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes Derived from the Human Primary Colorectal Cancer Cell Line SW480 Orchestrate Fibroblast-Led Cancer Invasion |
| Authors |
"Rai A, Greening DW, Xu R, Suwakulsiri W, Simpson RJ." |
| Journal name |
Proteomics
|
| Publication year |
2020 |
| Sample |
Colorectal cancer cells |
| Sample name |
SW480 - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 81 |
| Experiment ID |
327 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32438511
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomes Derived from the Human Primary Colorectal Cancer Cell Line SW480 Orchestrate Fibroblast-Led Cancer Invasion |
| Authors |
"Rai A, Greening DW, Xu R, Suwakulsiri W, Simpson RJ." |
| Journal name |
Proteomics
|
| Publication year |
2020 |
| Sample |
Colorectal cancer cells |
| Sample name |
SW480 - Fraction 7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
OptiPrep density gradient centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 82 |
| Experiment ID |
336 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|EPCAM|TSG101
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 83 |
| Experiment ID |
336 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|EPCAM|TSG101
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 84 |
| Experiment ID |
337 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD81|EPCAM|TSG101
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 85 |
| Experiment ID |
337 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD81|EPCAM|TSG101
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 86 |
| Experiment ID |
338 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 87 |
| Experiment ID |
339 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
miR-chip array |
|
|
| 88 |
| Experiment ID |
340 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
miR-chip array |
|
|
| 89 |
| Experiment ID |
341 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
miR-chip array |
|
|
| 90 |
| Experiment ID |
342 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|EPCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT29 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Mass spectrometry
miR-chip array |
|
|
| 91 |
| Experiment ID |
343 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|EPCAM
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT29 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Mass spectrometry
miR-chip array |
|
|
| 92 |
| Experiment ID |
344 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
qPCR |
|
|
| 93 |
| Experiment ID |
345 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
qPCR |
|
|
| 94 |
| Experiment ID |
346 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29472616
|
| Organism |
Homo sapiens |
| Experiment description |
Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246 |
| Authors |
"Cooks T, Pateras IS, Jenkins LM, Patel KM, Robles AI, Morris J, Forshew T, Appella E, Gorgoulis VG, Harris CC." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 - Fraction 7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
qPCR |
|
|
| 95 |
| Experiment ID |
399 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immunofluorescence
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Immunofluorescence
Flow cytomtery |
|
|
| 96 |
| Experiment ID |
399 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Immunofluorescence
Flow cytomtery |
|
|
| 97 |
| Experiment ID |
400 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Immunofluorescence
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Immunofluorescence
Flow cytomtery |
|
|
| 98 |
| Experiment ID |
400 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
33738083
|
| Organism |
Homo sapiens |
| Experiment description |
Bioorthogonally surface-edited extracellular vesicles based on metabolic glycoengineering for CD44-mediated targeting of inflammatory diseases |
| Authors |
"Lim GT, You DG, Han HS, Lee H, Shin S, Oh BH, Kumar EKP, Um W, Kim CH, Han S, Lee S, Lim S, Yoon HY, Kim K, Kwon IC, Jo DG, Cho YW, Park JH" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Immunofluorescence
Flow cytomtery |
|
|
| 99 |
| Experiment ID |
763 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35443745
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal miR-146a-5p and miR-155-5p promote CXCL12/CXCR7-induced metastasis of colorectal cancer by crosstalk with cancer-associated fibroblasts |
| Authors |
"Wang D, Wang X, Song Y, Si M, Sun Y, Liu X, Cui S, Qu X, Yu X." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Ultracentrifugation
ExoQuick Kit |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
RNA sequencing
RT-qPCR |
|
|
| 100 |
| Experiment ID |
764 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD81|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35443745
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal miR-146a-5p and miR-155-5p promote CXCL12/CXCR7-induced metastasis of colorectal cancer by crosstalk with cancer-associated fibroblasts |
| Authors |
"Wang D, Wang X, Song Y, Si M, Sun Y, Liu X, Cui S, Qu X, Yu X." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Ultracentrifugation
ExoQuick Kit |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
RNA sequencing
RT-qPCR |
|
|
| 101 |
| Experiment ID |
1202 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|CD9|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1215 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 102 |
| Experiment ID |
1203 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
DiFi |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RNA sequencing |
|
|
| 103 |
| Experiment ID |
1203 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
DiFi |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RNA sequencing |
|
|
| 104 |
| Experiment ID |
1203 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
DiFi |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RNA sequencing |
|
|
| 105 |
| Experiment ID |
1207 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|CD9|FLOT1
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCA-7 SC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 106 |
| Experiment ID |
1340 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63|FLOT1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36068649
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway |
| Authors |
"Wei X, Ye J, Pei Y, Wang C, Yang H, Tian J, Si G, Ma Y, Wang K, Liu G" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT29 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 107 |
| Experiment ID |
1341 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|CD63|FLOT1|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36068649
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles from colorectal cancer cells promote metastasis via the NOD1 signalling pathway |
| Authors |
"Wei X, Ye J, Pei Y, Wang C, Yang H, Tian J, Si G, Ma Y, Wang K, Liu G" |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HCT116 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 108 |
| Experiment ID |
1342 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36063142
|
| Organism |
Homo sapiens |
| Experiment description |
Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL |
| Authors |
"Gupta K, Brown KA, Hsieh ML, Hoover BM, Wang J, Khoury MK, Pilli VSS, Beyer RSH, Voruganti NR, Chaudhary S, Roberts DS, Murphy RM, Hong S, Ge Y, Liu B." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT-29 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 109 |
| Experiment ID |
1343 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36063142
|
| Organism |
Homo sapiens |
| Experiment description |
Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL |
| Authors |
"Gupta K, Brown KA, Hsieh ML, Hoover BM, Wang J, Khoury MK, Pilli VSS, Beyer RSH, Voruganti NR, Chaudhary S, Roberts DS, Murphy RM, Hong S, Ge Y, Liu B." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT-29 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 110 |
| Experiment ID |
1344 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36063142
|
| Organism |
Homo sapiens |
| Experiment description |
Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL |
| Authors |
"Gupta K, Brown KA, Hsieh ML, Hoover BM, Wang J, Khoury MK, Pilli VSS, Beyer RSH, Voruganti NR, Chaudhary S, Roberts DS, Murphy RM, Hong S, Ge Y, Liu B." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT-29 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 111 |
| Experiment ID |
1345 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36063142
|
| Organism |
Homo sapiens |
| Experiment description |
Necroptosis is associated with Rab27-independent expulsion of extracellular vesicles containing RIPK3 and MLKL |
| Authors |
"Gupta K, Brown KA, Hsieh ML, Hoover BM, Wang J, Khoury MK, Pilli VSS, Beyer RSH, Voruganti NR, Chaudhary S, Roberts DS, Murphy RM, Hong S, Ge Y, Liu B." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Colorectal cancer cells |
| Sample name |
HT-29 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 112 |
| Experiment ID |
117 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✘
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
21505438
|
| Organism |
Homo sapiens |
| Experiment description |
Unidirectional transfer of microRNA-loaded exosomes from T cells to antigen-presenting cells. |
| Authors |
"Mittelbrunn M, Gutierrez-Vázquez C, Villarroya-Beltri C, González S, Sánchez-Cabo F, González MÃÂ, Bernad A, Sánchez-Madrid F." |
| Journal name |
NATCOM
|
| Publication year |
2011 |
| Sample |
Dendritic cells |
| Sample name |
Dendritic cells |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Mass spectrometry
Microarray |
|
|
| 113 |
| Experiment ID |
407 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 114 |
| Experiment ID |
407 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 115 |
| Experiment ID |
419 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 116 |
| Experiment ID |
419 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 117 |
| Experiment ID |
420 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 118 |
| Experiment ID |
420 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 119 |
| Experiment ID |
452 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35932288
|
| Organism |
Homo sapiens |
| Experiment description |
Specific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides |
| Authors |
"Zhu Z, Zhai Y, Hao Y, Wang Q, Han F, Zheng W, Hong J, Cui L, Jin W, Ma S, Yang L, Cheng G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 120 |
| Experiment ID |
453 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35932288
|
| Organism |
Homo sapiens |
| Experiment description |
Specific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides |
| Authors |
"Zhu Z, Zhai Y, Hao Y, Wang Q, Han F, Zheng W, Hong J, Cui L, Jin W, Ma S, Yang L, Cheng G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 121 |
| Experiment ID |
454 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35932288
|
| Organism |
Homo sapiens |
| Experiment description |
Specific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides |
| Authors |
"Zhu Z, Zhai Y, Hao Y, Wang Q, Han F, Zheng W, Hong J, Cui L, Jin W, Ma S, Yang L, Cheng G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 122 |
| Experiment ID |
455 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35932288
|
| Organism |
Homo sapiens |
| Experiment description |
Specific anti-glioma targeted-delivery strategy of engineered small extracellular vesicles dual-functionalised by Angiopep-2 and TAT peptides |
| Authors |
"Zhu Z, Zhai Y, Hao Y, Wang Q, Han F, Zheng W, Hong J, Cui L, Jin W, Ma S, Yang L, Cheng G." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 123 |
| Experiment ID |
514 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|FLOT2
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36214496
|
| Organism |
Homo sapiens |
| Experiment description |
Contaminating transfection complexes can masquerade as small extracellular vesicles and impair their delivery of RNA |
| Authors |
"McCann J, Sosa-Miranda CD, Guo H, Reshke R, Savard A, Zardini Buzatto A, Taylor JA, Li L, Gibbings DJ." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293 - fraction 3-4 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 124 |
| Experiment ID |
923 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD81|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
30918259
|
| Organism |
Homo sapiens |
| Experiment description |
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A |
| Authors |
"Im EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 125 |
| Experiment ID |
1275 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
|
Enriched markers |
|
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 126 |
| Experiment ID |
1275 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
|
Enriched markers |
|
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 127 |
| Experiment ID |
1276 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 128 |
| Experiment ID |
1277 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 129 |
| Experiment ID |
1278 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9|TSG101
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 130 |
| Experiment ID |
1279 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 4 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.127 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 131 |
| Experiment ID |
1280 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
|
Enriched markers |
|
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.151 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 132 |
| Experiment ID |
1280 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
|
Enriched markers |
|
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.151 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 133 |
| Experiment ID |
1281 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.175 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 134 |
| Experiment ID |
1282 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 135 |
| Experiment ID |
1283 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 136 |
| Experiment ID |
1284 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 137 |
| Experiment ID |
1285 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 138 |
| Experiment ID |
1286 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 139 |
| Experiment ID |
1287 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Clone 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 140 |
| Experiment ID |
1288 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Clone 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 141 |
| Experiment ID |
1289 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 142 |
| Experiment ID |
1290 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 143 |
| Experiment ID |
1291 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 144 |
| Experiment ID |
1292 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 145 |
| Experiment ID |
1293 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 146 |
| Experiment ID |
1294 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.151 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 147 |
| Experiment ID |
1295 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.175 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 148 |
| Experiment ID |
1296 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 5 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.151 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 149 |
| Experiment ID |
1297 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Fraction 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
1.175 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 150 |
| Experiment ID |
1298 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36706192
|
| Organism |
Homo sapiens |
| Experiment description |
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles |
| Authors |
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q." |
| Journal name |
Sci Adv
|
| Publication year |
2023 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 151 |
| Experiment ID |
226 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
GAPDH|CD9|FLOT1
|
Enriched markers |
|
✔
LMNA|H2AFX|ATP5A1|TOMM20
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
26027894
|
| Organism |
Homo sapiens |
| Experiment description |
"Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" |
| Authors |
"Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" |
| Journal name |
Journal of Extracellular Vesicles
|
| Publication year |
2015 |
| Sample |
Endothelial cells |
| Sample name |
HMEC-1 |
| Isolation/purification methods |
Differential ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA |
| Methods used in the study |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting |
|
|
| 152 |
| Experiment ID |
305 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD81|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33431899
|
| Organism |
Homo sapiens |
| Experiment description |
High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance |
| Authors |
"Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Faeces |
| Sample name |
Healthy volunteers |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
Lipid |
| Methods used in the study |
Western blotting
Mass spectrometry
Immunofluorescence
HPLC |
|
|
| 153 |
| Experiment ID |
306 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD81|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33431899
|
| Organism |
Homo sapiens |
| Experiment description |
High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance |
| Authors |
"Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG." |
| Journal name |
Nat Commun
|
| Publication year |
2021 |
| Sample |
Faeces |
| Sample name |
Type 2 diabetes patients |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
Lipid |
| Methods used in the study |
Western blotting
Mass spectrometry
Immunofluorescence
HPLC |
|
|
| 154 |
| Experiment ID |
1086 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29438363
|
| Organism |
Homo sapiens |
| Experiment description |
CD9-positive exosomes from cancer-associated fibroblasts stimulate the migration ability of scirrhous-type gastric cancer cells |
| Authors |
"Miki Y, Yashiro M, Okuno T, Kitayama K, Masuda G, Hirakawa K, Ohira M." |
| Journal name |
Br J Cancer
|
| Publication year |
2018 |
| Sample |
Fibroblasts |
| Sample name |
CaF64 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 155 |
| Experiment ID |
1087 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29438363
|
| Organism |
Homo sapiens |
| Experiment description |
CD9-positive exosomes from cancer-associated fibroblasts stimulate the migration ability of scirrhous-type gastric cancer cells |
| Authors |
"Miki Y, Yashiro M, Okuno T, Kitayama K, Masuda G, Hirakawa K, Ohira M." |
| Journal name |
Br J Cancer
|
| Publication year |
2018 |
| Sample |
Fibroblasts |
| Sample name |
CaF65 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 156 |
| Experiment ID |
1220 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
31792209
|
| Organism |
Homo sapiens |
| Experiment description |
Eliminating blood oncogenic exosomes into the small intestine with aptamer-functionalized nanoparticles |
| Authors |
"Xie X, Nie H, Zhou Y, Lian S, Mei H, Lu Y, Dong H, Li F, Li T, Li B, Wang J, Lin M, Wang C, Shao J, Gao Y, Chen J, Xie F, Jia L." |
| Journal name |
Nat Commun
|
| Publication year |
2019 |
| Sample |
Fibroblasts |
| Sample name |
human embryonic lung fibroblast (HELF) |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Flow cytometry |
|
|
| 157 |
| Experiment ID |
1337 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35917363
|
| Organism |
Homo sapiens |
| Experiment description |
CD9 mediates the uptake of extracellular vesicles from cancer-associated fibroblasts that promote pancreatic cancer cell aggressiveness |
| Authors |
"Nigri J, Leca J, Tubiana SS, Finetti P, Guillaumond F, Martinez S, Lac S, Iovanna JL, Audebert S, Camoin L, Vasseur S, Bertucci F, Tomasini R." |
| Journal name |
Sci Signal
|
| Publication year |
2022 |
| Sample |
Fibroblasts |
| Sample name |
CAF - Pancreatic ductal adenocarcinoma |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 158 |
| Experiment ID |
1338 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35917363
|
| Organism |
Homo sapiens |
| Experiment description |
CD9 mediates the uptake of extracellular vesicles from cancer-associated fibroblasts that promote pancreatic cancer cell aggressiveness |
| Authors |
"Nigri J, Leca J, Tubiana SS, Finetti P, Guillaumond F, Martinez S, Lac S, Iovanna JL, Audebert S, Camoin L, Vasseur S, Bertucci F, Tomasini R." |
| Journal name |
Sci Signal
|
| Publication year |
2022 |
| Sample |
Fibroblasts |
| Sample name |
CAF - Pancreatic ductal adenocarcinoma |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 159 |
| Experiment ID |
1348 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
FLOT1|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36266345
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression |
| Authors |
"Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J." |
| Journal name |
Nat Commun
|
| Publication year |
2022 |
| Sample |
Fibroblasts |
| Sample name |
MRC-5 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 160 |
| Experiment ID |
1349 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
FLOT1|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36266345
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression |
| Authors |
"Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J." |
| Journal name |
Nat Commun
|
| Publication year |
2022 |
| Sample |
Fibroblasts |
| Sample name |
MRC-5 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 161 |
| Experiment ID |
1350 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
FLOT1|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36266345
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression |
| Authors |
"Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J." |
| Journal name |
Nat Commun
|
| Publication year |
2022 |
| Sample |
Fibroblasts |
| Sample name |
BJ |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 162 |
| Experiment ID |
1351 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
FLOT1|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36266345
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-produced and aging-associated oncometabolite methylmalonic acid promotes cancer-associated fibroblast activation to drive metastatic progression |
| Authors |
"Li Z, Low V, Luga V, Sun J, Earlie E, Parang B, Shobana Ganesh K, Cho S, Endress J, Schild T, Hu M, Lyden D, Jin W, Guo C, Dephoure N, Cantley LC, Laughney AM, Blenis J." |
| Journal name |
Nat Commun
|
| Publication year |
2022 |
| Sample |
Fibroblasts |
| Sample name |
BJ |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 163 |
| Experiment ID |
405 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Foreskin fibroblasts |
| Sample name |
BJ |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 164 |
| Experiment ID |
405 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Foreskin fibroblasts |
| Sample name |
BJ |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry |
|
|
| 165 |
| Experiment ID |
363 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
|
✔
DCLK1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 166 |
| Experiment ID |
364 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
|
✔
DCLK1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 167 |
| Experiment ID |
365 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
Enriched markers |
|
✔
DCLK1
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 168 |
| Experiment ID |
539 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35986010
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12 |
| Authors |
"Bao Z, Zhang N, Niu W, Mu M, Zhang X, Hu S, Niu C." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Glial cells |
| Sample name |
HEB |
| Isolation/purification methods |
Centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
miRNA sequencing |
|
|
| 169 |
| Experiment ID |
401 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63|CD81
|
Enriched markers |
|
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Glioblastoma |
| Sample name |
U87 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Flow cytometry |
|
|
| 170 |
| Experiment ID |
402 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R." |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Glioblastoma |
| Sample name |
U87 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
mRNA |
| Methods used in the study |
RT-qPCR
Flow cytometry |
|
|
| 171 |
| Experiment ID |
366 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33982667
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal circRNA 0001445 promotes glioma progression through miRNA-127-5p/SNX5 pathway |
| Authors |
"Han Y, Liu Y, Zhang B, Yin G." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Glioblastoma cells |
| Sample name |
A172 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 172 |
| Experiment ID |
367 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|ICAM1
|
Enriched markers |
|
✔
CANX
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
33982667
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal circRNA 0001445 promotes glioma progression through miRNA-127-5p/SNX5 pathway |
| Authors |
"Han Y, Liu Y, Zhang B, Yin G." |
| Journal name |
Aging
|
| Publication year |
2021 |
| Sample |
Glioblastoma cells |
| Sample name |
U373 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 173 |
| Experiment ID |
538 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
35986010
|
| Organism |
Homo sapiens |
| Experiment description |
Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12 |
| Authors |
"Bao Z, Zhang N, Niu W, Mu M, Zhang X, Hu S, Niu C." |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Glioblastoma cells |
| Sample name |
GSCs |
| Isolation/purification methods |
Centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
miRNA sequencing |
|
|
| 174 |
| Experiment ID |
691 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|CD63|TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29335551
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer |
| Authors |
"Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Liver cancer cells |
| Sample name |
CSQT-2 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray
qRT-PCR |
|
|
| 175 |
| Experiment ID |
692 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|CD63|TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29335551
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer |
| Authors |
"Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Liver cancer cells |
| Sample name |
HCC-LM3 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray
qRT-PCR |
|
|
| 176 |
| Experiment ID |
693 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|CD63|TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29335551
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer |
| Authors |
"Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Liver cancer cells |
| Sample name |
HepG2 |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray
qRT-PCR |
|
|
| 177 |
| Experiment ID |
694 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|CD63|TSG101|CD9|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Western blotting
|
| PubMed ID |
29335551
|
| Organism |
Homo sapiens |
| Experiment description |
Tumor-derived exosomal miR-1247-3p induces cancer-associated fibroblast activation to foster lung metastasis of liver cancer |
| Authors |
"Fang T, Lv H, Lv G, Li T, Wang C, Han Q, Yu L, Su B, Guo L, Huang S, Cao D, Tang L, Tang S, Wu M, Yang W, Wang H." |
| Journal name |
Nat Commun
|
| Publication year |
2018 |
| Sample |
Liver cancer cells |
| Sample name |
MHCC-97L |
| Isolation/purification methods |
Filtration
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Microarray
qRT-PCR |
|
|
| 178 |
| Experiment ID |
1371 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
PLC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 179 |
| Experiment ID |
1372 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
PLC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 180 |
| Experiment ID |
1373 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97L |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 181 |
| Experiment ID |
1374 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97L |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 182 |
| Experiment ID |
1375 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
MIHA |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 183 |
| Experiment ID |
1376 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
MIHA |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 184 |
| Experiment ID |
1377 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97H |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 185 |
| Experiment ID |
1378 |
| MISEV standards |
|
|
Biophysical techniques |
|
✔
CD81|CD63|CD9|TSG101|Alix
|
Enriched markers |
|
✘
|
Negative markers |
|
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36631249
|
| Organism |
Homo sapiens |
| Experiment description |
S100A10 promotes HCC development and progression via transfer in extracellular vesicles and regulating their protein cargos |
| Authors |
"Wang X, Huang H, Sze KM, Wang J, Tian L, Lu J, Tsui YM, Ma HT, Lee E, Chen A, Lee J, Wang Y, Yam JWP, Cheung TT, Guan X, Ng IO." |
| Journal name |
Gut
|
| Publication year |
2023 |
| Sample |
Liver cancer cells |
| Sample name |
97H |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 186 |
| Experiment ID |
328 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32132711
|
| Organism |
Homo sapiens |
| Experiment description |
Decoy exosomes provide protection against bacterial toxins |
| Authors |
"Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K." |
| Journal name |
Nature
|
| Publication year |
2020 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Flow cytometry |
|
|
| 187 |
| Experiment ID |
328 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
32132711
|
| Organism |
Homo sapiens |
| Experiment description |
Decoy exosomes provide protection against bacterial toxins |
| Authors |
"Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K." |
| Journal name |
Nature
|
| Publication year |
2020 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Flow cytometry |
|
|
| 188 |
| Experiment ID |
329 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32132711
|
| Organism |
Homo sapiens |
| Experiment description |
Decoy exosomes provide protection against bacterial toxins |
| Authors |
"Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K." |
| Journal name |
Nature
|
| Publication year |
2020 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Flow cytometry |
|
|
| 189 |
| Experiment ID |
329 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|CD81
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Flow cytometry
|
| PubMed ID |
32132711
|
| Organism |
Homo sapiens |
| Experiment description |
Decoy exosomes provide protection against bacterial toxins |
| Authors |
"Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K." |
| Journal name |
Nature
|
| Publication year |
2020 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Flow cytometry |
|
|
| 190 |
| Experiment ID |
330 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32132711
|
| Organism |
Homo sapiens |
| Experiment description |
Decoy exosomes provide protection against bacterial toxins |
| Authors |
"Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K." |
| Journal name |
Nature
|
| Publication year |
2020 |
| Sample |
Lung cancer cells |
| Sample name |
A549 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 191 |
| Experiment ID |
331 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32132711
|
| Organism |
Homo sapiens |
| Experiment description |
Decoy exosomes provide protection against bacterial toxins |
| Authors |
"Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K." |
| Journal name |
Nature
|
| Publication year |
2020 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 192 |
| Experiment ID |
332 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
32132711
|
| Organism |
Homo sapiens |
| Experiment description |
Decoy exosomes provide protection against bacterial toxins |
| Authors |
"Keller MD, Ching KL, Liang FX, Dhabaria A, Tam K, Ueberheide BM, Unutmaz D, Torres VJ, Cadwell K." |
| Journal name |
Nature
|
| Publication year |
2020 |
| Sample |
Lung cancer cells |
| Sample name |
A549 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 193 |
| Experiment ID |
546 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
CD9|CD63|GAPDH
|
Enriched markers |
|
✔
GOLGA2
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 194 |
| Experiment ID |
547 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 195 |
| Experiment ID |
548 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 196 |
| Experiment ID |
549 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 197 |
| Experiment ID |
550 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 198 |
| Experiment ID |
551 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 199 |
| Experiment ID |
554 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 200 |
| Experiment ID |
555 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 201 |
| Experiment ID |
556 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD9|CD63
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1299 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 202 |
| Experiment ID |
560 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 203 |
| Experiment ID |
561 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 204 |
| Experiment ID |
562 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 205 |
| Experiment ID |
563 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 206 |
| Experiment ID |
564 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 207 |
| Experiment ID |
565 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 208 |
| Experiment ID |
566 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
NTA
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
36482876
|
| Organism |
Homo sapiens |
| Experiment description |
Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels |
| Authors |
"Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H." |
| Journal name |
J Extracell Vesicles
|
| Publication year |
2022 |
| Sample |
Lung cancer cells |
| Sample name |
H1975 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting |
|
|
| 209 |
| Experiment ID |
567 |
| MISEV standards |
|
✘
|
Biophysical techniques |
|
✔
CD63|CD9
|
Enriched markers |
|
✘
|
Negative markers |
|
✔
| | | | | |