Gene description for USP12
Gene name ubiquitin specific peptidase 12
Gene symbol USP12
Other names/aliases UBH1
USP12L1
Species Homo sapiens
 Database cross references - USP12
ExoCarta ExoCarta_219333
Vesiclepedia VP_219333
Entrez Gene 219333
HGNC 20485
UniProt O75317  
 USP12 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for USP12
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 IMP
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 IDA
    cysteine-type deubiquitinase activity GO:0004843 TAS
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    proteolysis GO:0006508 IEA
    protein deubiquitination GO:0016579 IDA
    regulation of protein stability GO:0031647 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified USP12 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for USP12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 PHLPP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 HIST2H2BE 8349
Biochemical Activity Homo sapiens
5 PHLPP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 MECP2 4204
Affinity Capture-MS Homo sapiens
7 MAVS 57506
Affinity Capture-Western Homo sapiens
8 LAT 27040
Proximity Label-MS Homo sapiens
9 CBLB 868
Proximity Label-MS Homo sapiens
10 NUP160 23279
Affinity Capture-MS Homo sapiens
11 MMP2 4313
Affinity Capture-MS Homo sapiens
12 GRB2 2885
Proximity Label-MS Homo sapiens
13 WDR77 79084
Affinity Capture-MS Homo sapiens
14 USP46 64854
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 HMGB1 3146
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
16 CLASP2 23122
Affinity Capture-MS Homo sapiens
17 SGOL1  
Affinity Capture-MS Homo sapiens
18 RNF19B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TSPYL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 IGDCC4  
Affinity Capture-MS Homo sapiens
21 PPM1B 5495
Affinity Capture-Western Homo sapiens
22 RAD51AP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 OPALIN  
Affinity Capture-MS Homo sapiens
24 VSX2 338917
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CD6 923
Affinity Capture-MS Homo sapiens
26 CX3CL1  
Affinity Capture-MS Homo sapiens
27 YWHAB 7529
Affinity Capture-MS Homo sapiens
28 TRAT1 50852
Proximity Label-MS Homo sapiens
29 UBC 7316
Reconstituted Complex Homo sapiens
30 DMWD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 GPR161  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 WDR26 80232
Affinity Capture-MS Homo sapiens
33 APP 351
Reconstituted Complex Homo sapiens
34 DHX8 1659
Affinity Capture-MS Homo sapiens
35 GORASP1 64689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 WDR48 57599
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
37 HSPB9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 HIST2H2AC 8338
Biochemical Activity Homo sapiens
39 WDR20 91833
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
40 USP1 7398
Affinity Capture-MS Homo sapiens
41 USP39 10713
Affinity Capture-MS Homo sapiens
42 ZAP70 7535
Proximity Label-MS Homo sapiens
43 PNMA2  
Affinity Capture-MS Homo sapiens
44 EPHA2 1969
Proximity Label-MS Homo sapiens
45 EP300 2033
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
46 AR 367
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
47 YWHAG 7532
Affinity Capture-MS Homo sapiens
48 PHF8  
Affinity Capture-MS Homo sapiens
49 ITCH 83737
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
50 MDK 4192
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 NOTCH1 4851
Biochemical Activity Homo sapiens
52 HSPB8 26353
Affinity Capture-MS Homo sapiens
53 SDF4 51150
Affinity Capture-MS Homo sapiens
54 VSX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 UCHL5 51377
Affinity Capture-MS Homo sapiens
56 PAIP1 10605
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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