Gene description for EIF2AK4
Gene name eukaryotic translation initiation factor 2 alpha kinase 4
Gene symbol EIF2AK4
Other names/aliases GCN2
PVOD2
Species Homo sapiens
 Database cross references - EIF2AK4
ExoCarta ExoCarta_440275
Vesiclepedia VP_440275
Entrez Gene 440275
HGNC 19687
MIM 609280
UniProt Q9P2K8  
 EIF2AK4 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for EIF2AK4
Molecular Function
    tRNA binding GO:0000049 ISS
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IBA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IDA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IMP
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 ISS
    ribosomal protein S6 kinase activity GO:0004711 IEA
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    DNA damage checkpoint signaling GO:0000077 IEA
    positive regulation of defense response to virus by host GO:0002230 ISS
    adaptive immune response GO:0002250 IEA
    T cell activation involved in immune response GO:0002286 ISS
    positive regulation of adaptive immune response GO:0002821 ISS
    chromatin remodeling GO:0006338 IEA
    regulation of translational initiation GO:0006446 ISS
    protein phosphorylation GO:0006468 ISS
    learning GO:0007612 ISS
    long-term memory GO:0007616 ISS
    regulation of translational initiation by eIF2 alpha phosphorylation GO:0010998 IMP
    regulation of translational initiation by eIF2 alpha phosphorylation GO:0010998 ISS
    viral translation GO:0019081 ISS
    negative regulation of translational initiation in response to stress GO:0032057 IBA
    negative regulation of translational initiation in response to stress GO:0032057 ISS
    negative regulation of CREB transcription factor activity GO:0032792 ISS
    cellular response to amino acid starvation GO:0034198 IBA
    cellular response to amino acid starvation GO:0034198 IMP
    cellular response to UV GO:0034644 ISS
    eiF2alpha phosphorylation in response to endoplasmic reticulum stress GO:0036492 IMP
    negative regulation by host of viral genome replication GO:0044828 ISS
    negative regulation of neuron differentiation GO:0045665 ISS
    negative regulation of translational initiation GO:0045947 ISS
    protein autophosphorylation GO:0046777 ISS
    defense response to virus GO:0051607 IEA
    regulation of feeding behavior GO:0060259 ISS
    cellular response to cold GO:0070417 IMP
    positive regulation of translational initiation in response to starvation GO:0071264 ISS
    GCN2-mediated signaling GO:0140469 IDA
    GCN2-mediated signaling GO:0140469 IMP
    GCN2-mediated signaling GO:0140469 ISS
    positive regulation of long-term synaptic potentiation GO:1900273 ISS
    neuron projection extension GO:1990138 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IBA
    cytosolic ribosome GO:0022626 ISS
 Experiment description of studies that identified EIF2AK4 in exosomes
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for EIF2AK4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FTL 2512
Affinity Capture-MS Homo sapiens
2 ARRB2 409
Affinity Capture-MS Homo sapiens
3 TMPO 7112
Affinity Capture-MS Homo sapiens
4 FAM153B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 Edc4  
Affinity Capture-MS Mus musculus
6 S100P 6286
Affinity Capture-MS Homo sapiens
7 PSMC3 5702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 DHDH  
Affinity Capture-MS Homo sapiens
9 USP46 64854
Affinity Capture-MS Homo sapiens
10 KIF14 9928
Affinity Capture-MS Homo sapiens
11 PRPS2 5634
Affinity Capture-MS Homo sapiens
12 CIB2  
Affinity Capture-MS Homo sapiens
13 DNAJA2 10294
Affinity Capture-MS Homo sapiens
14 GGNBP1  
Two-hybrid Homo sapiens
15 PPP1CC 5501
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PPP1CA 5499
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 EN1  
Affinity Capture-MS Homo sapiens
18 HDHD1  
Affinity Capture-MS Homo sapiens
19 PGM2L1 283209
Affinity Capture-MS Homo sapiens
20 SRRM1 10250
Cross-Linking-MS (XL-MS) Homo sapiens
21 TGM2 7052
Affinity Capture-MS Homo sapiens
22 TKT 7086
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PALM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 KLHL34  
Affinity Capture-MS Homo sapiens
25 MAGEA9  
Affinity Capture-MS Homo sapiens
26 MDM4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TARS 6897
Co-fractionation Homo sapiens
28 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
29 TRAP1 10131
Affinity Capture-Western Homo sapiens
30 CROT 54677
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 C6orf141  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PPP4R1L  
Affinity Capture-MS Homo sapiens
33 Sept11  
Affinity Capture-MS Mus musculus
34 CDC16 8881
Affinity Capture-MS Homo sapiens
35 SREBF2 6721
Negative Genetic Homo sapiens
36 LRPPRC 10128
Affinity Capture-MS Homo sapiens
37 METTL21B  
Affinity Capture-MS Homo sapiens
38 CRYL1 51084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 ALDH3B1 221
Affinity Capture-MS Homo sapiens
40 FBXW7  
Affinity Capture-MS Homo sapiens
41 LDLR 3949
Positive Genetic Homo sapiens
42 MATR3 9782
Affinity Capture-MS Homo sapiens
43 NUP210P1  
Affinity Capture-MS Homo sapiens
44 UBXN6 80700
Affinity Capture-MS Homo sapiens
45 DUSP16  
Affinity Capture-MS Homo sapiens
46 DEF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 AK9  
Affinity Capture-MS Homo sapiens
48 PIPSL 266971
Affinity Capture-MS Homo sapiens
49 CAPN2 824
Affinity Capture-MS Homo sapiens
50 KLHL14 57565
Affinity Capture-MS Homo sapiens
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