Gene description for CASP9
Gene name caspase 9, apoptosis-related cysteine peptidase
Gene symbol CASP9
Other names/aliases APAF-3
APAF3
ICE-LAP6
MCH6
PPP1R56
Species Homo sapiens
 Database cross references - CASP9
ExoCarta ExoCarta_842
Vesiclepedia VP_842
Entrez Gene 842
HGNC 1511
MIM 602234
UniProt P55211  
 CASP9 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CASP9
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 IBA
    cysteine-type endopeptidase activity GO:0004197 IDA
    cysteine-type endopeptidase activity GO:0004197 IMP
    cysteine-type endopeptidase activity GO:0004197 TAS
    protein binding GO:0005515 IPI
    enzyme activator activity GO:0008047 TAS
    peptidase activity GO:0008233 IDA
    SH3 domain binding GO:0017124 IDA
    protein kinase binding GO:0019901 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    response to hypoxia GO:0001666 IEA
    kidney development GO:0001822 IEA
    response to ischemia GO:0002931 IEA
    apoptotic process GO:0006915 IBA
    apoptotic process GO:0006915 IMP
    apoptotic process GO:0006915 TAS
    DNA damage response GO:0006974 IDA
    DNA damage response GO:0006974 IMP
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IBA
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IMP
    protein processing GO:0016485 IEA
    platelet formation GO:0030220 TAS
    response to cobalt ion GO:0032025 IEA
    response to estradiol GO:0032355 IEA
    response to lipopolysaccharide GO:0032496 IEA
    glial cell apoptotic process GO:0034349 IEA
    cellular response to UV GO:0034644 IDA
    signal transduction in response to DNA damage GO:0042770 IDA
    positive regulation of apoptotic process GO:0043065 IMP
    positive regulation of neuron apoptotic process GO:0043525 IBA
    fibroblast apoptotic process GO:0044346 IEA
    neuron apoptotic process GO:0051402 IEA
    protein maturation GO:0051604 IDA
    cellular response to dexamethasone stimulus GO:0071549 IEA
    leukocyte apoptotic process GO:0071887 IEA
    intrinsic apoptotic signaling pathway GO:0097193 IC
    intrinsic apoptotic signaling pathway GO:0097193 IDA
    intrinsic apoptotic signaling pathway GO:0097193 NAS
    epithelial cell apoptotic process GO:1904019 IEA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 TAS
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    caspase complex GO:0008303 IPI
    protein-containing complex GO:0032991 IDA
    apoptosome GO:0043293 IDA
    apoptosome GO:0043293 IPI
 Experiment description of studies that identified CASP9 in sEVs
1
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CASP9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 SLFN11 91607
Affinity Capture-MS Homo sapiens
3 APAF1 317
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 BIRC2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
5 HSD17B4 3295
Two-hybrid Homo sapiens
6 XIAP  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 RNF34  
Affinity Capture-Western Homo sapiens
8 BAX 581
Co-fractionation Homo sapiens
9 CASP7  
Biochemical Activity Homo sapiens
10 MAPK1 5594
Biochemical Activity Homo sapiens
11 CRYAB 1410
Affinity Capture-Western Homo sapiens
12 CASP8 841
Biochemical Activity Homo sapiens
Phenotypic Suppression Homo sapiens
Biochemical Activity Homo sapiens
13 CASP2  
Phenotypic Suppression Homo sapiens
14 SQSTM1 8878
Affinity Capture-Western Homo sapiens
15 ABL1 25
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 NAIP  
Far Western Homo sapiens
17 MSTO1 55154
Two-hybrid Homo sapiens
18 JUN 3725
Biochemical Activity Homo sapiens
19 BIRC6 57448
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
20 BCL2L1 598
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
21 VCP 7415
Affinity Capture-Western Homo sapiens
22 NOD1 10392
Affinity Capture-Western Homo sapiens
23 CASP3 836
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
24 CASP10  
Biochemical Activity Homo sapiens
25 NLRP1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 DCC  
Affinity Capture-Western Homo sapiens
27 CASP12 100506742
Biochemical Activity Homo sapiens
28 BICD2 23299
Proximity Label-MS Homo sapiens
29 RNF2  
Biochemical Activity Homo sapiens
30 CYCS 54205
Co-localization Homo sapiens
31 BIRC7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
32 BIRC8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
33 PTCH1  
Affinity Capture-Western Homo sapiens
34 BIRC5  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
35 ATG7 10533
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 BIRC3 330
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
View the network image/svg+xml
 Pathways in which CASP9 is involved
PathwayEvidenceSource
Activation of caspases through apoptosome-mediated cleavage TAS Reactome
AKT phosphorylates targets in the cytosol TAS Reactome
Apoptosis TAS Reactome
Apoptosis IEA Reactome
Apoptotic factor-mediated response TAS Reactome
Apoptotic factor-mediated response IEA Reactome
Caspase activation via Dependence Receptors in the absence of ligand TAS Reactome
Caspase activation via extrinsic apoptotic signalling pathway TAS Reactome
Constitutive Signaling by AKT1 E17K in Cancer TAS Reactome
Cytochrome c-mediated apoptotic response TAS Reactome
Cytochrome c-mediated apoptotic response IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Formation of apoptosome TAS Reactome
Formation of apoptosome IEA Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Intracellular signaling by second messengers TAS Reactome
Intrinsic Pathway for Apoptosis TAS Reactome
Intrinsic Pathway for Apoptosis IEA Reactome
NOD1/2 Signaling Pathway TAS Reactome
Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PIP3 activates AKT signaling TAS Reactome
Programmed Cell Death TAS Reactome
Programmed Cell Death IEA Reactome
Regulation of the apoptosome activity IEA Reactome
Regulation of the apoptosome activity TAS Reactome
Signal Transduction TAS Reactome
SMAC (DIABLO) binds to IAPs IEA Reactome
SMAC(DIABLO)-mediated dissociation of IAP:caspase complexes IEA Reactome
SMAC, XIAP-regulated apoptotic response IEA Reactome





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