Gene description for CASP9
Gene name caspase 9, apoptosis-related cysteine peptidase
Gene symbol CASP9
Other names/aliases APAF-3
APAF3
ICE-LAP6
MCH6
PPP1R56
Species Homo sapiens
 Database cross references - CASP9
ExoCarta ExoCarta_842
Entrez Gene 842
HGNC 1511
MIM 602234
UniProt P55211  
 CASP9 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
 Gene ontology annotations for CASP9
Molecular Function
    cysteine-type endopeptidase activity GO:0004197 TAS
    protein binding GO:0005515 IPI
    cysteine-type endopeptidase activity involved in apoptotic signaling pathway GO:0097199 IBA
    peptidase activity GO:0008233 IDA
    protein kinase binding GO:0019901 IDA
    SH3 domain binding GO:0017124 IDA
    enzyme activator activity GO:0008047 TAS
Biological Process
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IMP
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    response to cobalt ion GO:0032025 IEA
    programmed cell death GO:0012501 TAS
    response to estradiol GO:0032355 IEA
    cellular response to DNA damage stimulus GO:0006974 IDA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    extrinsic apoptotic signaling pathway GO:0097191 TAS
    aging GO:0007568 IEA
    signal transduction in response to DNA damage GO:0042770 IDA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    cellular response to UV GO:0034644 IDA
    glial cell apoptotic process GO:0034349 IEA
    positive regulation of neuron apoptotic process GO:0043525 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    response to antibiotic GO:0046677 IEA
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    response to lipopolysaccharide GO:0032496 IEA
    cellular response to dexamethasone stimulus GO:0071549 IEA
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 TAS
    regulation of response to DNA damage stimulus GO:2001020 IMP
    innate immune response GO:0045087 TAS
    platelet formation GO:0030220 TAS
    apoptotic process GO:0006915 TAS
    activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c GO:0008635 TAS
Subcellular Localization
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    apoptosome GO:0043293 IDA
 Experiment description of studies that identified CASP9 in exosomes
1
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 81
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
 Protein-protein interactions for CASP9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Biochemical Activity Homo sapiens
2 DCC  
Affinity Capture-Western Homo sapiens
3 NOD1 10392
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
4 GZMB  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
5 BIRC2  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 BCL2L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
7 CYCS  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
8 BIRC7  
Affinity Capture-Western Homo sapiens
9 XIAP  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
10 CASP9 842
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 BIRC3  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 APAF1 317
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 BIRC1  
Far Western Homo sapiens
14 NALP1  
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
15 BIRC8  
Reconstituted Complex Homo sapiens
16 BIRC5  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
17 CASP10  
Invitro Homo sapiens
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