Gene description for PGAP1
Gene name post-GPI attachment to proteins 1
Gene symbol PGAP1
Other names/aliases Bst1
ISPD3024
MRT42
SPG67
Species Homo sapiens
 Database cross references - PGAP1
ExoCarta ExoCarta_80055
Vesiclepedia VP_80055
Entrez Gene 80055
HGNC 25712
MIM 611655
UniProt Q75T13  
 PGAP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PGAP1
Molecular Function
    hydrolase activity, acting on ester bonds GO:0016788 TAS
    phosphatidylinositol deacylase activity GO:0050185 IBA
    phosphatidylinositol deacylase activity GO:0050185 ISS
Biological Process
    GPI anchor biosynthetic process GO:0006506 IBA
    sensory perception of sound GO:0007605 IEA
    embryonic pattern specification GO:0009880 IEA
    anterior/posterior axis specification GO:0009948 IEA
    protein transport GO:0015031 IEA
    attachment of GPI anchor to protein GO:0016255 TAS
    forebrain regionalization GO:0021871 IEA
    positive regulation of ER to Golgi vesicle-mediated transport GO:1902953 ISS
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified PGAP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PGAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NRG1 3084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CLGN 1047
Affinity Capture-MS Homo sapiens
3 CHRNA4  
Affinity Capture-MS Homo sapiens
4 TTYH1  
Affinity Capture-MS Homo sapiens
5 CANX 821
Affinity Capture-MS Homo sapiens
6 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 MCOLN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HTR3C  
Affinity Capture-MS Homo sapiens
9 ATP1B4  
Affinity Capture-MS Homo sapiens
10 TECTA 7007
Affinity Capture-MS Homo sapiens
11 PLTP 5360
Affinity Capture-MS Homo sapiens
12 MAP1LC3A 84557
Affinity Capture-MS Homo sapiens
13 CHRNB4  
Affinity Capture-MS Homo sapiens
14 NCEH1 57552
Affinity Capture-MS Homo sapiens
15 TCTN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PTCHD3  
Affinity Capture-MS Homo sapiens
17 HYOU1 10525
Affinity Capture-MS Homo sapiens
18 ISLR 3671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 UPK1A 11045
Affinity Capture-MS Homo sapiens
20 SIRT3  
Affinity Capture-MS Homo sapiens
21 BTNL8  
Affinity Capture-MS Homo sapiens
22 NXPE2  
Affinity Capture-MS Homo sapiens
23 PON2 5445
Affinity Capture-MS Homo sapiens
24 ITM2B 9445
Affinity Capture-MS Homo sapiens
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