Gene description for ARFGAP2
Gene name ADP-ribosylation factor GTPase activating protein 2
Gene symbol ARFGAP2
Other names/aliases IRZ
NBLA10535
ZFP289
ZNF289
Species Homo sapiens
 Database cross references - ARFGAP2
ExoCarta ExoCarta_84364
Vesiclepedia VP_84364
Entrez Gene 84364
HGNC 13504
MIM 606908
UniProt Q8N6H7  
 ARFGAP2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for ARFGAP2
Molecular Function
    GTPase activator activity GO:0005096 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    protein transport GO:0015031 IEA
    COPI coating of Golgi vesicle GO:0048205 IBA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified ARFGAP2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 434
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 435
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARFGAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 PXMP2  
Proximity Label-MS Homo sapiens
4 HOOK1  
Proximity Label-MS Homo sapiens
5 HSPA2 3306
Affinity Capture-MS Homo sapiens
6 ASF1A 25842
Affinity Capture-MS Homo sapiens
7 ACRBP  
Affinity Capture-MS Homo sapiens
8 EBAG9 9166
Proximity Label-MS Homo sapiens
9 MAD2L1 4085
Affinity Capture-MS Homo sapiens
10 ARF4 378
Proximity Label-MS Homo sapiens
11 FAM20C 56975
Affinity Capture-MS Homo sapiens
12 METTL7A 25840
Proximity Label-MS Homo sapiens
13 MARCKS 4082
Proximity Label-MS Homo sapiens
14 HSPA8 3312
Affinity Capture-MS Homo sapiens
15 XPO1 7514
Affinity Capture-MS Homo sapiens
16 GJA1 2697
Proximity Label-MS Homo sapiens
17 UNC45A 55898
Affinity Capture-MS Homo sapiens
18 SERPING1 710
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TSSK6 83983
Affinity Capture-MS Homo sapiens
20 RAB5A 5868
Proximity Label-MS Homo sapiens
21 EIF2AK2 5610
Co-fractionation Homo sapiens
22 ARF5 381
Proximity Label-MS Homo sapiens
23 GOLGA1  
Proximity Label-MS Homo sapiens
24 ARFGAP3 26286
Affinity Capture-MS Homo sapiens
25 PHKA2 5256
Affinity Capture-MS Homo sapiens
26 MIB1 57534
Proximity Label-MS Homo sapiens
27 TSG101 7251
Co-fractionation Homo sapiens
28 ARF3 377
Proximity Label-MS Homo sapiens
29 BAG2 9532
Affinity Capture-MS Homo sapiens
30 SQSTM1 8878
Proximity Label-MS Homo sapiens
31 ADI1 55256
Co-fractionation Homo sapiens
32 EMD 2010
Proximity Label-MS Homo sapiens
33 HSD17B10 3028
Co-fractionation Homo sapiens
34 OCM2  
Affinity Capture-MS Homo sapiens
35 HSD3B7 80270
Proximity Label-MS Homo sapiens
36 DCAF6 55827
Affinity Capture-MS Homo sapiens
37 STX4 6810
Proximity Label-MS Homo sapiens
38 DDX58 23586
Affinity Capture-RNA Homo sapiens
39 MKI67  
Affinity Capture-MS Homo sapiens
40 UBE2I 7329
Co-fractionation Homo sapiens
41 ESR1  
Affinity Capture-MS Homo sapiens
42 JAZF1  
Affinity Capture-MS Homo sapiens
43 MRPS18C  
Affinity Capture-MS Homo sapiens
44 PFN1 5216
Proximity Label-MS Homo sapiens
45 ERGIC2 51290
Proximity Label-MS Homo sapiens
46 MAPK6  
Affinity Capture-MS Homo sapiens
47 PRPH 5630
Proximity Label-MS Homo sapiens
48 TCF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 ZBTB2 57621
Affinity Capture-MS Homo sapiens
50 CDH1 999
Proximity Label-MS Homo sapiens
51 SEC61B 10952
Proximity Label-MS Homo sapiens
52 RPA3 6119
Proximity Label-MS Homo sapiens
53 BBS1 582
Affinity Capture-MS Homo sapiens
54 VCP 7415
Affinity Capture-MS Homo sapiens
55 FAM24B  
Affinity Capture-MS Homo sapiens
56 DUSP16  
Affinity Capture-MS Homo sapiens
57 B3GAT1  
Proximity Label-MS Homo sapiens
58 NUP153 9972
Co-fractionation Homo sapiens
59 ARF1 375
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
60 HSPA1A 3303
Affinity Capture-MS Homo sapiens
61 ANKRD49  
Affinity Capture-MS Homo sapiens
62 VRK2 7444
Affinity Capture-MS Homo sapiens
63 BICD2 23299
Proximity Label-MS Homo sapiens
64 RPA4  
Proximity Label-MS Homo sapiens
65 ACTB 60
Proximity Label-MS Homo sapiens
66 BCAR3 8412
Affinity Capture-MS Homo sapiens
67 ACTR1A 10121
Proximity Label-MS Homo sapiens
68 MILR1  
Affinity Capture-MS Homo sapiens
69 CLK3  
Affinity Capture-MS Homo sapiens
70 CCDC96  
Affinity Capture-MS Homo sapiens
71 SSR1 6745
Proximity Label-MS Homo sapiens
72 ATP5F1 515
Affinity Capture-MS Homo sapiens
73 DERL1 79139
Proximity Label-MS Homo sapiens
74 KDM3A  
Co-fractionation Homo sapiens
75 CAV1 857
Proximity Label-MS Homo sapiens
76 ELOVL5 60481
Proximity Label-MS Homo sapiens
77 CEPT1 10390
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 STX6 10228
Proximity Label-MS Homo sapiens
79 ARFGAP1 55738
Co-fractionation Homo sapiens
80 TATDN1 83940
Co-fractionation Homo sapiens
81 AHR 196
Affinity Capture-MS Homo sapiens
82 ATP2A1 487
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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