Gene description for TMEM67
Gene name transmembrane protein 67
Gene symbol TMEM67
Other names/aliases JBTS6
MECKELIN
MKS3
NPHP11
TNEM67
Species Homo sapiens
 Database cross references - TMEM67
ExoCarta ExoCarta_91147
Vesiclepedia VP_91147
Entrez Gene 91147
HGNC 28396
MIM 609884
UniProt Q5HYA8  
 TMEM67 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TMEM67
Molecular Function
    protein binding GO:0005515 IPI
    filamin binding GO:0031005 IPI
    unfolded protein binding GO:0051082 IPI
Biological Process
    negative regulation of centrosome duplication GO:0010826 IMP
    non-canonical Wnt signaling pathway GO:0035567 IMP
    ERAD pathway GO:0036503 IMP
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 IMP
    cilium assembly GO:0060271 ISS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IDA
    centrosome GO:0005813 IDA
    cytoplasmic vesicle membrane GO:0030659 IDA
    ciliary transition zone GO:0035869 IBA
    ciliary transition zone GO:0035869 IDA
    MKS complex GO:0036038 ISS
    ciliary membrane GO:0060170 IDA
    ciliary membrane GO:0060170 TAS
 Experiment description of studies that identified TMEM67 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TMEM67
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 LYZL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 UPK2 7379
Affinity Capture-MS Homo sapiens
4 CST5 1473
Affinity Capture-MS Homo sapiens
5 HTR3A  
Affinity Capture-MS Homo sapiens
6 HSD17B11 51170
Proximity Label-MS Homo sapiens
7 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HSPA5 3309
Affinity Capture-Western Homo sapiens
9 BTNL2  
Affinity Capture-MS Homo sapiens
10 WFDC6  
Affinity Capture-MS Homo sapiens
11 TMEM30A 55754
Affinity Capture-MS Homo sapiens
12 CLGN 1047
Affinity Capture-MS Homo sapiens
13 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
14 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SIGLECL1  
Affinity Capture-MS Homo sapiens
16 HLA-G 3135
Affinity Capture-MS Homo sapiens
17 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 NDP  
Affinity Capture-MS Homo sapiens
19 DEFB109P1B  
Affinity Capture-MS Homo sapiens
20 IGFL1 374918
Affinity Capture-MS Homo sapiens
21 TMPRSS11B 132724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 DUOXA2  
Affinity Capture-MS Homo sapiens
23 HYOU1 10525
Affinity Capture-MS Homo sapiens
24 GP9 2815
Affinity Capture-MS Homo sapiens
25 CD1B 910
Affinity Capture-MS Homo sapiens
26 SCGB1D4  
Affinity Capture-MS Homo sapiens
27 CD300E  
Affinity Capture-MS Homo sapiens
28 ADAM33 80332
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 DEFB110  
Affinity Capture-MS Homo sapiens
30 ASIC4  
Affinity Capture-MS Homo sapiens
31 CD70 970
Affinity Capture-MS Homo sapiens
32 CFC1  
Affinity Capture-MS Homo sapiens
33 FAM19A4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 DEFB121  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 TMEM30B 161291
Affinity Capture-MS Homo sapiens
36 CD1A 909
Affinity Capture-MS Homo sapiens
37 ATP1B4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 CUL3 8452
Affinity Capture-MS Homo sapiens
39 ITM2C 81618
Affinity Capture-MS Homo sapiens
40 SYNE2 23224
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
41 HLA-DRA 3122
Affinity Capture-MS Homo sapiens
42 HPN  
Affinity Capture-MS Homo sapiens
43 CD79A  
Affinity Capture-MS Homo sapiens
44 PSCA 8000
Affinity Capture-MS Homo sapiens
45 SPINK4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SLC22A9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ELSPBP1  
Affinity Capture-MS Homo sapiens
48 SFTPC  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
49 CD27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 DEFB136  
Affinity Capture-MS Homo sapiens
51 MSMB  
Affinity Capture-MS Homo sapiens
52 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
53 TM2D3  
Affinity Capture-MS Homo sapiens
54 BRICD5  
Affinity Capture-MS Homo sapiens
55 HLA-DRB3 3125
Affinity Capture-MS Homo sapiens
56 CST11  
Affinity Capture-MS Homo sapiens
57 CD1E  
Affinity Capture-MS Homo sapiens
58 CDHR4  
Affinity Capture-MS Homo sapiens
59 CLEC2B  
Affinity Capture-MS Homo sapiens
60 FAM19A5 25817
Affinity Capture-MS Homo sapiens
61 DEFB135  
Affinity Capture-MS Homo sapiens
62 DEFB123  
Affinity Capture-MS Homo sapiens
63 TCTN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 ATP1B1 481
Affinity Capture-MS Homo sapiens
65 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 GML  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 MUC1 4582
Affinity Capture-MS Homo sapiens
68 NRN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 FAM19A2 338811
Affinity Capture-MS Homo sapiens
70 ST14 6768
Affinity Capture-MS Homo sapiens
71 HLA-DRB1 3123
Affinity Capture-MS Homo sapiens
72 HLA-C 3107
Affinity Capture-MS Homo sapiens
73 CANX 821
Affinity Capture-MS Homo sapiens
74 SDF2L1 23753
Affinity Capture-MS Homo sapiens
75 PATE1  
Affinity Capture-MS Homo sapiens
76 APOM 55937
Affinity Capture-MS Homo sapiens
77 IGFL3  
Affinity Capture-MS Homo sapiens
78 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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