Gene description for NUP54
Gene name nucleoporin 54kDa
Gene symbol NUP54
Other names/aliases -
Species Homo sapiens
 Database cross references - NUP54
ExoCarta ExoCarta_53371
Vesiclepedia VP_53371
Entrez Gene 53371
HGNC 17359
MIM 607607
UniProt Q7Z3B4  
 NUP54 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NUP54
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of nuclear pore GO:0017056 IBA
    identical protein binding GO:0042802 IEA
    protein-containing complex binding GO:0044877 IEA
Biological Process
    protein targeting GO:0006605 IEA
    NLS-bearing protein import into nucleus GO:0006607 IBA
    nucleocytoplasmic transport GO:0006913 NAS
    nuclear pore organization GO:0006999 IBA
    protein localization to nuclear inner membrane GO:0036228 IBA
    regulation of protein import into nucleus GO:0042306 IEA
    mRNA transport GO:0051028 IEA
Subcellular Localization
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 TAS
    nuclear pore GO:0005643 NAS
    nuclear membrane GO:0031965 IEA
    nuclear pore central transport channel GO:0044613 IBA
 Experiment description of studies that identified NUP54 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NUP54
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HOMER3 9454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 BPIFA1 51297
Two-hybrid Homo sapiens
3 AP2B1 163
Two-hybrid Homo sapiens
4 Cul1 26965
Affinity Capture-MS Mus musculus
5 ETV6  
Proximity Label-MS Homo sapiens
6 KPNA1 3836
Reconstituted Complex Homo sapiens
7 NUP62 23636
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
8 KPNB1 3837
Reconstituted Complex Homo sapiens
9 APC  
Two-hybrid Homo sapiens
10 NUPL1 9818
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
11 SPSB4  
Affinity Capture-MS Homo sapiens
12 C9orf78 51759
Affinity Capture-MS Homo sapiens
13 TIGD6  
Affinity Capture-MS Homo sapiens
14 TRIM54  
Two-hybrid Homo sapiens
15 NDE1 54820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 EXOC1 55763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 C21orf91  
Two-hybrid Homo sapiens
18 NUP153 9972
Co-fractionation Homo sapiens
19 SMYD3 64754
Two-hybrid Homo sapiens
20 HGS 9146
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
21 CLIP1 6249
Affinity Capture-MS Homo sapiens
22 ADD1 118
Affinity Capture-MS Homo sapiens
23 OIP5  
Two-hybrid Homo sapiens
24 OBSL1 23363
Affinity Capture-MS Homo sapiens
25 TEX12  
Two-hybrid Homo sapiens
26 ZWINT  
Two-hybrid Homo sapiens
27 IFT20 90410
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 NUP155 9631
Proximity Label-MS Homo sapiens
29 NFKBID  
Two-hybrid Homo sapiens
30 XPO1 7514
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CPNE2 221184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CHMP7 91782
Affinity Capture-MS Homo sapiens
33 CENPH  
Two-hybrid Homo sapiens
34 PARK2  
Affinity Capture-MS Homo sapiens
35 MAPRE1 22919
Affinity Capture-MS Homo sapiens
36 HAUS8  
Two-hybrid Homo sapiens
37 VCP 7415
Affinity Capture-MS Homo sapiens
38 NTRK1 4914
Affinity Capture-MS Homo sapiens
39 RNF166  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 TCHP  
Two-hybrid Homo sapiens
41 AGR2 10551
Two-hybrid Homo sapiens
42 GDF3 9573
Affinity Capture-MS Homo sapiens
43 PKNOX2  
Two-hybrid Homo sapiens
44 COG4 25839
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 OGT 8473
Reconstituted Complex Homo sapiens
46 RAN 5901
Affinity Capture-MS Homo sapiens
47 TEKT1  
Two-hybrid Homo sapiens
48 NUMA1 4926
Affinity Capture-MS Homo sapiens
49 DCTN3 11258
Two-hybrid Homo sapiens
50 Nup98  
Affinity Capture-MS Mus musculus
51 KRT33B 3884
Two-hybrid Homo sapiens
52 NUP62CL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 C14orf119  
Two-hybrid Homo sapiens
54 NUP214 8021
Affinity Capture-MS Homo sapiens
55 HOMER1  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
56 KRT27 342574
Two-hybrid Homo sapiens
57 KRT15 3866
Two-hybrid Homo sapiens
58 CEP250 11190
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 Nup107  
Affinity Capture-MS Mus musculus
60 SOHLH1  
Two-hybrid Homo sapiens
61 CCDC85B  
Two-hybrid Homo sapiens
62 DGCR6  
Two-hybrid Homo sapiens
63 RANBP1 5902
Affinity Capture-MS Homo sapiens
64 Fign  
Affinity Capture-MS Mus musculus
65 RAE1 8480
Co-fractionation Homo sapiens
66 TPM3 7170
Two-hybrid Homo sapiens
67 CRNN 49860
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 EIF3F 8665
Two-hybrid Homo sapiens
69 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
70 NUTF2 10204
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
71 NUP205 23165
Cross-Linking-MS (XL-MS) Homo sapiens
72 WDFY2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 HAUS1  
Two-hybrid Homo sapiens
74 EXOC8 149371
Two-hybrid Homo sapiens
75 VPS37C 55048
Two-hybrid Homo sapiens
76 CCHCR1  
Two-hybrid Homo sapiens
77 BFSP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 HDAC1 3065
Affinity Capture-Western Homo sapiens
79 NUP35 129401
Proximity Label-MS Homo sapiens
80 NUP98 4928
Co-fractionation Homo sapiens
81 LPXN 9404
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 NUP107 57122
Affinity Capture-MS Homo sapiens
83 NEDD1 121441
Affinity Capture-MS Homo sapiens
84 C1orf109  
Two-hybrid Homo sapiens
85 CIT 11113
Affinity Capture-MS Homo sapiens
86 SERPINB2 5055
Affinity Capture-MS Homo sapiens
87 NDEL1 81565
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 WDR59 79726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 KRAS 3845
Negative Genetic Homo sapiens
90 SPEN 23013
Co-fractionation Homo sapiens
91 CDK16 5127
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NUP54 is involved
PathwayEvidenceSource
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic TAS Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Defective TPR may confer susceptibility towards thyroid papillary carcinoma (TPC) TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Disorders of transmembrane transporters TAS Reactome
Export of Viral Ribonucleoproteins from Nucleus TAS Reactome
Gene expression (Transcription) TAS Reactome
Gene Silencing by RNA TAS Reactome
Glucose metabolism TAS Reactome
Glycolysis TAS Reactome
HCMV Early Events TAS Reactome
HCMV Infection TAS Reactome
HCMV Late Events TAS Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle TAS Reactome
Host Interactions of HIV factors IEA Reactome
Host Interactions of HIV factors TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Influenza Infection TAS Reactome
Influenza Viral RNA Transcription and Replication TAS Reactome
Interactions of Rev with host cellular proteins IEA Reactome
Interactions of Rev with host cellular proteins TAS Reactome
Interactions of Vpr with host cellular proteins TAS Reactome
Interferon Signaling TAS Reactome
ISG15 antiviral mechanism TAS Reactome
Late Phase of HIV Life Cycle TAS Reactome
M Phase TAS Reactome
M Phase IEA Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome
Metabolism of non-coding RNA TAS Reactome
Metabolism of proteins TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA TAS Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prophase TAS Reactome
NEP/NS2 Interacts with the Cellular Export Machinery TAS Reactome
NS1 Mediated Effects on Host Pathways TAS Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Nuclear Envelope Breakdown TAS Reactome
Nuclear import of Rev protein IEA Reactome
Nuclear Pore Complex (NPC) Disassembly TAS Reactome
Post-translational protein modification TAS Reactome
Post-translational protein modification IEA Reactome
Postmitotic nuclear pore complex (NPC) reformation IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
Regulation of Glucokinase by Glucokinase Regulatory Protein TAS Reactome
Regulation of HSF1-mediated heat shock response TAS Reactome
Rev-mediated nuclear export of HIV RNA TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-2 activates/modulates innate and adaptive immune responses TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
SLC transporter disorders TAS Reactome
snRNP Assembly TAS Reactome
SUMO E3 ligases SUMOylate target proteins TAS Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation TAS Reactome
SUMOylation IEA Reactome
SUMOylation of chromatin organization proteins TAS Reactome
SUMOylation of DNA damage response and repair proteins TAS Reactome
SUMOylation of DNA replication proteins IEA Reactome
SUMOylation of DNA replication proteins TAS Reactome
SUMOylation of RNA binding proteins TAS Reactome
SUMOylation of SUMOylation proteins TAS Reactome
SUMOylation of ubiquitinylation proteins TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript TAS Reactome
Transport of Mature mRNA Derived from an Intronless Transcript TAS Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts TAS Reactome
Transport of Mature Transcript to Cytoplasm TAS Reactome
Transport of Ribonucleoproteins into the Host Nucleus TAS Reactome
Transport of the SLBP Dependant Mature mRNA TAS Reactome
Transport of the SLBP independent Mature mRNA TAS Reactome
tRNA processing TAS Reactome
tRNA processing in the nucleus TAS Reactome
Viral Infection Pathways TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Messenger RNA Synthesis TAS Reactome
Vpr-mediated nuclear import of PICs TAS Reactome





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