Gene description for IFT88
Gene name intraflagellar transport 88
Gene symbol IFT88
Other names/aliases D13S1056E
DAF19
TG737
TTC10
hTg737
Species Homo sapiens
 Database cross references - IFT88
ExoCarta ExoCarta_8100
Vesiclepedia VP_8100
Entrez Gene 8100
HGNC 20606
MIM 600595
UniProt Q13099  
 IFT88 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IFT88
Molecular Function
    protein binding GO:0005515 IPI
    kinesin binding GO:0019894 IBA
Biological Process
    kidney development GO:0001822 IBA
    response to silicon dioxide GO:0034021 IEA
    intraciliary anterograde transport GO:0035720 NAS
    intraciliary transport GO:0042073 IBA
    positive regulation of cilium assembly GO:0045724 IMP
    inner ear receptor cell stereocilium organization GO:0060122 IBA
    cilium assembly GO:0060271 ISS
    cilium assembly GO:0060271 NAS
    regulation of cilium assembly GO:1902017 ISS
    non-motile cilium assembly GO:1905515 IBA
    regulation of autophagosome assembly GO:2000785 ISS
Subcellular Localization
    manchette GO:0002177 ISS
    centrosome GO:0005813 IDA
    centriole GO:0005814 IBA
    centriole GO:0005814 IDA
    centriole GO:0005814 ISS
    cytosol GO:0005829 TAS
    cilium GO:0005929 IDA
    cilium GO:0005929 ISS
    cilium GO:0005929 NAS
    cilium GO:0005929 TAS
    intraciliary transport particle B GO:0030992 IPI
    intraciliary transport particle B GO:0030992 ISS
    motile cilium GO:0031514 ISS
    ciliary basal body GO:0036064 IBA
    ciliary basal body GO:0036064 IDA
    ciliary basal body GO:0036064 ISS
    sperm flagellum GO:0036126 ISS
    ciliary tip GO:0097542 TAS
    ciliary base GO:0097546 IBA
    non-motile cilium GO:0097730 IBA
    sperm head-tail coupling apparatus GO:0120212 ISS
 Experiment description of studies that identified IFT88 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IFT88
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IFT80 57560
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
2 ZWINT  
Affinity Capture-MS Homo sapiens
3 CCDC151  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 IFT27 11020
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 TTC30A 92104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 Cdk1 12534
Affinity Capture-MS Mus musculus
7 ICK  
Affinity Capture-MS Homo sapiens
8 PRRC2A 7916
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 TTC26 79989
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
10 Myh9 17886
Affinity Capture-MS Mus musculus
11 Tmed10 68581
Affinity Capture-MS Mus musculus
12 IFT20 90410
Affinity Capture-MS Homo sapiens
13 CLUAP1 23059
Affinity Capture-MS Homo sapiens
14 TFPI2 7980
Affinity Capture-MS Homo sapiens
15 UBXN10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
16 Dennd6a  
Affinity Capture-MS Mus musculus
17 TRAF3IP1 26146
Affinity Capture-MS Homo sapiens
18 HSPB11 51668
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CDKN2AIP  
Affinity Capture-MS Homo sapiens
20 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SMC6  
Affinity Capture-MS Homo sapiens
22 FKBP4 2288
Cross-Linking-MS (XL-MS) Homo sapiens
23 VHL  
Two-hybrid Homo sapiens
24 Rock1  
Affinity Capture-MS Mus musculus
25 TTC30B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 Tuba3a 22144
Affinity Capture-MS Mus musculus
27 ABTB2 25841
Affinity Capture-MS Homo sapiens
28 IFT46 56912
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 VCP 7415
Affinity Capture-MS Homo sapiens
30 FGF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
32 IFT52 51098
Affinity Capture-MS Homo sapiens
33 HNRNPAB 3182
Cross-Linking-MS (XL-MS) Homo sapiens
34 IFT172 26160
Affinity Capture-MS Homo sapiens
35 Junb  
Affinity Capture-MS Mus musculus
36 ZC2HC1C  
Affinity Capture-MS Homo sapiens
37 IFT81 28981
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
38 IFT22 64792
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 IFT74 80173
Affinity Capture-MS Homo sapiens
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