Gene description for WASF1
Gene name WAS protein family, member 1
Gene symbol WASF1
Other names/aliases SCAR1
WAVE
WAVE1
Species Homo sapiens
 Database cross references - WASF1
ExoCarta ExoCarta_8936
Vesiclepedia VP_8936
Entrez Gene 8936
HGNC 12732
MIM 605035
UniProt Q92558  
 WASF1 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for WASF1
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IMP
    protein kinase A regulatory subunit binding GO:0034237 IBA
    protein kinase A binding GO:0051018 IPI
    Arp2/3 complex binding GO:0071933 IBA
Biological Process
    receptor-mediated endocytosis GO:0006898 ISS
    mitochondrion organization GO:0007005 IMP
    Rac protein signal transduction GO:0016601 IMP
    actin cytoskeleton organization GO:0030036 IBA
    actin cytoskeleton organization GO:0030036 IMP
    actin filament polymerization GO:0030041 TAS
    positive regulation of neurotrophin TRK receptor signaling pathway GO:0051388 IEA
    protein-containing complex assembly GO:0065003 NAS
    lamellipodium morphogenesis GO:0072673 IMP
    dendrite extension GO:0097484 IEA
    modification of postsynaptic actin cytoskeleton GO:0098885 IEA
    cellular response to brain-derived neurotrophic factor stimulus GO:1990416 ISS
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 IBA
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 IMP
Subcellular Localization
    mitochondrial outer membrane GO:0005741 IEA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 TAS
    focal adhesion GO:0005925 IEA
    actin cytoskeleton GO:0015629 TAS
    lamellipodium GO:0030027 IBA
    SCAR complex GO:0031209 IBA
    SCAR complex GO:0031209 IMP
    protein-containing complex GO:0032991 IDA
    postsynapse GO:0098794 IEA
 Experiment description of studies that identified WASF1 in exosomes
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WASF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 CSNK1G3 1456
Affinity Capture-MS Homo sapiens
3 WASF2 10163
Affinity Capture-MS Homo sapiens
4 TIMM50 92609
Affinity Capture-MS Homo sapiens
5 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 FNBP1L 54874
Co-fractionation Homo sapiens
7 NCK1 4690
Affinity Capture-MS Homo sapiens
8 WASF3 10810
Affinity Capture-MS Homo sapiens
9 NYAP2  
Reconstituted Complex Homo sapiens
10 MAPKAPK2 9261
Reconstituted Complex Homo sapiens
11 CGB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 AIPL1  
Affinity Capture-MS Homo sapiens
13 IFT20 90410
Two-hybrid Homo sapiens
14 NR3C1 2908
Proximity Label-MS Homo sapiens
15 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 NTRK1 4914
Affinity Capture-MS Homo sapiens
17 ACTG1 71
Affinity Capture-MS Homo sapiens
18 PFN1 5216
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
19 ZNF746  
Affinity Capture-MS Homo sapiens
20 YWHAE 7531
Affinity Capture-MS Homo sapiens
21 ARHGAP12  
Affinity Capture-MS Homo sapiens
22 RYK 6259
Affinity Capture-MS Homo sapiens
23 ZNF511  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ABL1 25
Affinity Capture-Western Homo sapiens
25 CDH23 64072
Affinity Capture-MS Homo sapiens
26 SORBS2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 UBXN11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 DNMBP 23268
Affinity Capture-MS Homo sapiens
29 CYFIP2 26999
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
30 NHSL1  
Affinity Capture-MS Homo sapiens
31 Nhsl1  
Affinity Capture-MS Mus musculus
32 TXNDC9 10190
Co-fractionation Homo sapiens
33 ABI3 51225
Two-hybrid Homo sapiens
34 LINC00238  
Two-hybrid Homo sapiens
35 ATP6V1C2 245973
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 LMO2  
Two-hybrid Homo sapiens
37 MPDZ  
Affinity Capture-MS Homo sapiens
38 ABI2 10152
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 NCKAP1 10787
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 TCF4  
Affinity Capture-MS Homo sapiens
41 PTPRE 5791
Proximity Label-MS Homo sapiens
42 YWHAZ 7534
Affinity Capture-MS Homo sapiens
43 AMZ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PTPN12 5782
Affinity Capture-Western Homo sapiens
45 ING5  
Two-hybrid Homo sapiens
46 ACTR3 10096
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
47 GRB2 2885
Two-hybrid Homo sapiens
48 OSTF1 26578
Two-hybrid Homo sapiens
49 TRIP10 9322
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
50 TRAF2 7186
Two-hybrid Homo sapiens
51 KIAA1522 57648
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 CYFIP1 23191
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
53 CCDC53 51019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 ABI1 10006
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 HSF2BP  
Two-hybrid Homo sapiens
57 BRK1 55845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 ZC3H15 55854
Co-fractionation Homo sapiens
59 BAIAP2 10458
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
60 RHOB 388
Proximity Label-MS Homo sapiens
61 NXN 64359
Affinity Capture-MS Homo sapiens
62 CBLC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 NHSL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 WASL 8976
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which WASF1 is involved
PathwayEvidenceSource
Disease TAS Reactome
Disease IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis TAS Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
FCGR3A-mediated phagocytosis TAS Reactome
FCGR3A-mediated phagocytosis IEA Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
Leishmania infection TAS Reactome
Leishmania infection IEA Reactome
Leishmania phagocytosis TAS Reactome
Leishmania phagocytosis IEA Reactome
Parasite infection TAS Reactome
Parasite infection IEA Reactome
Parasitic Infection Pathways TAS Reactome
Parasitic Infection Pathways IEA Reactome
RAC1 GTPase cycle TAS Reactome
RAC3 GTPase cycle TAS Reactome
Regulation of actin dynamics for phagocytic cup formation TAS Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
RHO GTPase cycle TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate WASPs and WAVEs TAS Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by VEGF TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome





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