Gene description for Kif23
Gene name kinesin family member 23
Gene symbol Kif23
Other names/aliases 3110001D19Rik
C87313
CHO1
Knsl5
MKLP-1
MKLP1
Species Mus musculus
 Database cross references - Kif23
ExoCarta ExoCarta_71819
Vesiclepedia VP_71819
Entrez Gene 71819
UniProt E9Q5G3  
 Kif23 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Kif23
Molecular Function
    microtubule motor activity GO:0003777 IBA
    microtubule motor activity GO:0003777 IEA
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IEA
    microtubule binding GO:0008017 ISO
    ATP hydrolysis activity GO:0016887 IBA
Biological Process
    mitotic cytokinesis GO:0000281 ISO
    mitotic cytokinesis GO:0000281 ISS
    microtubule-based movement GO:0007018 IBA
    microtubule-based movement GO:0007018 IEA
    positive regulation of cytokinesis GO:0032467 ISO
    mitotic spindle midzone assembly GO:0051256 IBA
    mitotic spindle midzone assembly GO:0051256 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    centrosome GO:0005813 ISO
    spindle GO:0005819 ISO
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    microtubule GO:0005874 IEA
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    intercellular bridge GO:0045171 IDA
    mitotic spindle GO:0072686 IEA
    mitotic spindle GO:0072686 ISO
    Flemming body GO:0090543 IEA
    Flemming body GO:0090543 ISO
    centralspindlin complex GO:0097149 ISO
 Experiment description of studies that identified Kif23 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Kif23
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRR14L 253143
Affinity Capture-MS Homo sapiens
2 CCAR1 55749
Affinity Capture-MS Homo sapiens
3 Cenpi  
Co-fractionation Mus musculus
4 Pde4dip  
Affinity Capture-MS Mus musculus
5 Fbxo5  
Co-fractionation Mus musculus
6 Prr11  
Co-fractionation Mus musculus
7 Aspm  
Co-fractionation Mus musculus
8 IFT20 90410
Affinity Capture-MS Homo sapiens
9 Smc2 14211
Co-fractionation Mus musculus
10 PAWR 5074
Affinity Capture-MS Homo sapiens
11 1110037F02Rik 66185
Co-fractionation Mus musculus
12 RACGAP1 29127
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 Twist1  
Proximity Label-MS Mus musculus
14 TRAF3IP1 26146
Affinity Capture-MS Homo sapiens
15 CD2AP 23607
Affinity Capture-MS Homo sapiens
16 Nup93  
Co-fractionation Mus musculus
17 Tex14  
Co-fractionation Mus musculus
18 Ncaph  
Co-fractionation Mus musculus
19 LATS1  
Biochemical Activity Homo sapiens
20 PHB2 11331
Affinity Capture-MS Homo sapiens
21 Cobll1 319876
Co-fractionation Mus musculus
22 MICAL3 57553
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 KIF23 9493
Affinity Capture-MS Homo sapiens
24 Cenpk  
Co-fractionation Mus musculus
25 Mki67 17345
Co-fractionation Mus musculus
26 Smarca5 93762
Co-fractionation Mus musculus
27 SHCBP1 79801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 Cenpu  
Co-fractionation Mus musculus
29 Smc4 70099
Co-fractionation Mus musculus
30 LATS2 26524
Biochemical Activity Homo sapiens
31 Zwilch  
Co-fractionation Mus musculus
View the network image/svg+xml



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