Gene description for PCLO
Gene name piccolo presynaptic cytomatrix protein
Gene symbol PCLO
Other names/aliases ACZ
Species Homo sapiens
 Database cross references - PCLO
ExoCarta ExoCarta_27445
Vesiclepedia VP_27445
Entrez Gene 27445
HGNC 13406
MIM 604918
UniProt Q9Y6V0  
 PCLO identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
 Gene ontology annotations for PCLO
Molecular Function
    calcium ion binding GO:0005509 ISS
    profilin binding GO:0005522 ISS
    calcium-dependent phospholipid binding GO:0005544 ISS
    structural constituent of presynaptic active zone GO:0098882 IBA
Biological Process
    cytoskeleton organization GO:0007010 ISS
    synaptic vesicle exocytosis GO:0016079 NAS
    regulation of exocytosis GO:0017157 IEA
    insulin secretion GO:0030073 IEA
    protein localization to synapse GO:0035418 IBA
    maintenance of presynaptic active zone structure GO:0048790 IEA
    synaptic vesicle clustering GO:0097091 IEA
    presynaptic actin cytoskeleton organization GO:0099140 IEA
    presynaptic active zone assembly GO:1904071 IBA
Subcellular Localization
    cytoskeleton GO:0005856 NAS
    postsynaptic density GO:0014069 IEA
    axon GO:0030424 IBA
    synapse GO:0045202 ISS
    cytoskeleton of presynaptic active zone GO:0048788 IBA
    cytoskeleton of presynaptic active zone GO:0048788 TAS
    extracellular exosome GO:0070062 HDA
    glutamatergic synapse GO:0098978 IBA
    GABA-ergic synapse GO:0098982 IBA
 Experiment description of studies that identified PCLO in sEVs
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
3
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for PCLO
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST1H2BL 8340
Cross-Linking-MS (XL-MS) Homo sapiens
2 OTC 5009
Proximity Label-MS Homo sapiens
3 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
4 KIF14 9928
Affinity Capture-MS Homo sapiens
5 EIF3C 8663
Cross-Linking-MS (XL-MS) Homo sapiens
6 KCTD10 83892
Affinity Capture-MS Homo sapiens
7 PDIA4 9601
Cross-Linking-MS (XL-MS) Homo sapiens
8 HIST2H2BF 440689
Cross-Linking-MS (XL-MS) Homo sapiens
9 GIT2 9815
Two-hybrid Homo sapiens
10 ERC2 26059
Affinity Capture-Western Homo sapiens
11 HIST1H2BJ 8970
Cross-Linking-MS (XL-MS) Homo sapiens
12 DPPA2  
Cross-Linking-MS (XL-MS) Homo sapiens
13 KIF20A 10112
Affinity Capture-MS Homo sapiens
14 THRAP3 9967
Cross-Linking-MS (XL-MS) Homo sapiens
15 LOC102724334 102724334
Cross-Linking-MS (XL-MS) Homo sapiens
16 CTBP1 1487
Proximity Label-MS Homo sapiens
17 ECE1 1889
Cross-Linking-MS (XL-MS) Homo sapiens
18 HIST1H2BO 8348
Cross-Linking-MS (XL-MS) Homo sapiens
19 PSMD14 10213
Affinity Capture-MS Homo sapiens
20 UFL1 23376
Affinity Capture-MS Homo sapiens
21 DPPA4  
Affinity Capture-MS Homo sapiens
22 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
23 TMEM132D  
Two-hybrid Homo sapiens
24 GIT1 28964
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
25 DUOX2 50506
Cross-Linking-MS (XL-MS) Homo sapiens
26 KIF23 9493
Affinity Capture-MS Homo sapiens
27 RPS6 6194
Cross-Linking-MS (XL-MS) Homo sapiens
28 HNRNPAB 3182
Cross-Linking-MS (XL-MS) Homo sapiens
29 FBXW8 26259
Affinity Capture-MS Homo sapiens
30 OBSL1 23363
Affinity Capture-MS Homo sapiens
31 CEP72  
Affinity Capture-MS Homo sapiens
32 SURF1  
Proximity Label-MS Homo sapiens
33 HCLS1 3059
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml



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