Gene description for L1CAM
Gene name L1 cell adhesion molecule
Gene symbol L1CAM
Other names/aliases CAML1
CD171
HSAS
HSAS1
MASA
MIC5
N-CAM-L1
N-CAML1
NCAM-L1
S10
SPG1
Species Homo sapiens
 Database cross references - L1CAM
ExoCarta ExoCarta_3897
Entrez Gene 3897
HGNC 6470
MIM 308840
UniProt P32004  
 L1CAM identified in exosomes derived from the following tissue/cell type
Glioma cells 19109410    
Malignant ascites 19188015    
Medulloblastoma cells 19109410    
Melanoma cells 25950383    
Melanoma cells 25950383    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for L1CAM
Molecular Function
    integrin binding GO:0005178 IEA
    sialic acid binding GO:0033691 IEA
    protein self-association GO:0043621 IEA
    identical protein binding GO:0042802 IEA
Biological Process
    homophilic cell adhesion via plasma membrane adhesion molecules GO:0007156 IEA
    homotypic cell-cell adhesion GO:0034109 IEA
    cell-cell adhesion mediated by integrin GO:0033631 IEA
    blood coagulation GO:0007596 TAS
    positive regulation of calcium-mediated signaling GO:0050850 IEA
    nervous system development GO:0007399 TAS
    leukocyte cell-cell adhesion GO:0007159 IEA
    leukocyte migration GO:0050900 TAS
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 IEA
    cell adhesion GO:0007155 NAS
    chemotaxis GO:0006935 TAS
    cell surface receptor signaling pathway GO:0007166 IEA
    axon guidance GO:0007411 TAS
    positive regulation of cell-cell adhesion GO:0022409 IEA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 IDA
    cell surface GO:0009986 IDA
    presynaptic membrane GO:0042734 IEA
    integral component of membrane GO:0016021 IEA
    terminal bouton GO:0043195 IEA
    external side of plasma membrane GO:0009897 IEA
 Experiment description of studies that identified L1CAM in exosomes
1
Experiment ID 28
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSC70
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Homo sapiens
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Glioma cells
Sample name X43,T
D456MG
H2159MG
D54MG
D247MG
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
2
Experiment ID 131
ISEV standards
✘ EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
EpCAM|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19188015    
Organism Homo sapiens
Experiment description Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P.
Journal name CL
Publication year 2009
Sample Malignant ascites
Sample name Malignant ascites - Ovarian cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 mg/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Cytofluorographic analysis
Annexin-V-FITC
Western blotting
FACS
3
Experiment ID 131
ISEV standards
✘ EV Biophysical techniques
✔
HSP70
EV Cytosolic markers
✔
EpCAM|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19188015    
Organism Homo sapiens
Experiment description Systemic presence and tumor-growth promoting effect of ovarian carcinoma released exosomes
Authors Keller S, Konig AK, Marme F, Runz S, Wolterink S, Koensgen D, Mustea A, Sehouli J, Altevogt P.
Journal name CL
Publication year 2009
Sample Malignant ascites
Sample name Malignant ascites - Ovarian cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.08-1.14 mg/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Cytofluorographic analysis
Annexin-V-FITC
Western blotting
FACS
4
Experiment ID 27
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19109410    
Organism Homo sapiens
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Medulloblastoma cells
Sample name D283MED
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
5
Experiment ID 259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for L1CAM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANK2  
Invivo Homo sapiens
2 ITGA5 3678
Reconstituted Complex Homo sapiens
3 EZR 7430
Reconstituted Complex Homo sapiens
4 ITGAV 3685
Reconstituted Complex Homo sapiens
5 AP2A1 160
Affinity Capture-Western Homo sapiens
6 NCAM1 4684
Invitro Homo sapiens
7 ANK1 286
Invivo Homo sapiens
8 NUMB 8650
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 PEA15  
Two-hybrid Homo sapiens
10 CSPG3  
Reconstituted Complex Homo sapiens
11 CNTN2  
Invitro Homo sapiens
12 CNTN1 1272
Invivo Homo sapiens
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