Gene description for EEA1
Gene name early endosome antigen 1
Gene symbol EEA1
Other names/aliases MST105
MSTP105
ZFYVE2
Species Homo sapiens
 Database cross references - EEA1
ExoCarta ExoCarta_8411
Entrez Gene 8411
HGNC 3185
MIM 605070
UniProt Q15075  
 EEA1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for EEA1
Molecular Function
    1-phosphatidylinositol binding GO:0005545 IDA
    calmodulin binding GO:0005516 NAS
    zinc ion binding GO:0008270 TAS
    GTP-dependent protein binding GO:0030742 IDA
    protein homodimerization activity GO:0042803 IDA
    protein binding GO:0005515 IPI
Biological Process
    vesicle fusion GO:0006906 IMP
    synaptic vesicle to endosome fusion GO:0016189 TAS
    endocytosis GO:0006897 IMP
    early endosome to late endosome transport GO:0045022 NAS
Subcellular Localization
    extrinsic component of plasma membrane GO:0019897 IDA
    cytosol GO:0005829 IDA
    cytoplasm GO:0005737 TAS
    extracellular exosome GO:0070062 IDA
    cytoplasmic vesicle GO:0031410 IEA
    axonal spine GO:0044308 IEA
    membrane GO:0016020 TAS
    early endosome membrane GO:0031901 IEA
    serine-pyruvate aminotransferase complex GO:0005969 IEA
    early endosome GO:0005769 IDA
    recycling endosome GO:0055037 IEA
 Experiment description of studies that identified EEA1 in exosomes
1
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for EEA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB22A 57403
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
2 RAB5C 5878
Invitro Homo sapiens
3 RABEP1  
Co-fractionation Homo sapiens
4 RAB5B 5869
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 STX12 23673
Reconstituted Complex Homo sapiens
6 NSF 4905
Co-fractionation Homo sapiens
7 EEA1 8411
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
8 RAB5A 5868
FRET Homo sapiens
View the network image/svg+xml
 Pathways in which EEA1 is involved
PathwayEvidenceSource
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome





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