Gene description for SEC24A
Gene name SEC24 family member A
Gene symbol SEC24A
Other names/aliases -
Species Homo sapiens
 Database cross references - SEC24A
ExoCarta ExoCarta_10802
Vesiclepedia VP_10802
Entrez Gene 10802
HGNC 10703
MIM 607183
UniProt O95486  
 SEC24A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SEC24A
Molecular Function
    SNARE binding GO:0000149 IBA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 IDA
Biological Process
    intracellular protein transport GO:0006886 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IMP
    regulation of cholesterol transport GO:0032374 IEA
    cholesterol homeostasis GO:0042632 IEA
    positive regulation of protein secretion GO:0050714 IEA
    COPII-coated vesicle cargo loading GO:0090110 IBA
    COPII-coated vesicle cargo loading GO:0090110 IDA
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    cytosol GO:0005829 TAS
    ER to Golgi transport vesicle membrane GO:0012507 TAS
    COPII vesicle coat GO:0030127 IBA
    COPII vesicle coat GO:0030127 IDA
    endoplasmic reticulum exit site GO:0070971 IBA
 Experiment description of studies that identified SEC24A in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SEC24A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRG 5793
Proximity Label-MS Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 MAN2B1 4125
Co-fractionation Homo sapiens
4 STAT5B 6777
Co-fractionation Homo sapiens
5 METTL7A 25840
Proximity Label-MS Homo sapiens
6 LAMP3  
Proximity Label-MS Homo sapiens
7 PDAP1 11333
Co-fractionation Homo sapiens
8 GJA1 2697
Proximity Label-MS Homo sapiens
9 S100A6 6277
Co-fractionation Homo sapiens
10 SEPHS2  
Co-fractionation Homo sapiens
11 TLE3  
Co-fractionation Homo sapiens
12 ROCK2 9475
Co-fractionation Homo sapiens
13 KIAA1598 57698
Co-fractionation Homo sapiens
14 ILF2 3608
Co-fractionation Homo sapiens
15 VPS26A 9559
Co-fractionation Homo sapiens
16 GTF2H2C  
Co-fractionation Homo sapiens
17 PARK2  
Affinity Capture-MS Homo sapiens
18 B3GAT1  
Proximity Label-MS Homo sapiens
19 C18orf25 147339
Co-fractionation Homo sapiens
20 TSNAX 7257
Co-fractionation Homo sapiens
21 SEC24C 9632
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
22 CREB1  
Co-fractionation Homo sapiens
23 SSR1 6745
Proximity Label-MS Homo sapiens
24 EWSR1 2130
Two-hybrid Homo sapiens
25 EPS15L1 58513
Co-fractionation Homo sapiens
26 SLC25A46 91137
Proximity Label-MS Homo sapiens
27 ARID5A  
Two-hybrid Homo sapiens
28 GTSE1 51512
Affinity Capture-MS Homo sapiens
29 SEC23A 10484
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
30 SNX27 81609
Affinity Capture-MS Homo sapiens
31 EBAG9 9166
Proximity Label-MS Homo sapiens
32 STMN2  
Co-fractionation Homo sapiens
33 NUP155 9631
Proximity Label-MS Homo sapiens
34 KIAA1715 80856
Proximity Label-MS Homo sapiens
35 PANX1 24145
Proximity Label-MS Homo sapiens
36 PRDX5 25824
Co-fractionation Homo sapiens
37 UCN3  
Affinity Capture-MS Homo sapiens
38 EMD 2010
Proximity Label-MS Homo sapiens
39 RUFY1 80230
Co-fractionation Homo sapiens
40 SRA1 10011
Affinity Capture-MS Homo sapiens
41 CXADR 1525
Proximity Label-MS Homo sapiens
42 CLNS1A 1207
Co-fractionation Homo sapiens
43 FAM118B  
Co-fractionation Homo sapiens
44 PSMD2 5708
Co-fractionation Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 PRRC1 133619
Affinity Capture-MS Homo sapiens
47 TTC9C 283237
Co-fractionation Homo sapiens
48 CSTB 1476
Co-fractionation Homo sapiens
49 ELOVL5 60481
Proximity Label-MS Homo sapiens
50 DNM1L 10059
Co-fractionation Homo sapiens
51 SEC61B 10952
Proximity Label-MS Homo sapiens
52 ASAP2 8853
Co-fractionation Homo sapiens
53 IGBP1 3476
Co-fractionation Homo sapiens
54 TRIP13 9319
Two-hybrid Homo sapiens
55 KIF1B 23095
Co-fractionation Homo sapiens
56 CBFA2T3  
Two-hybrid Homo sapiens
57 AHCYL1 10768
Co-fractionation Homo sapiens
58 XPO1 7514
Affinity Capture-MS Homo sapiens
59 NUBP2 10101
Co-fractionation Homo sapiens
60 SEC24D 9871
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
61 USP16  
Co-fractionation Homo sapiens
62 HSD3B7 80270
Proximity Label-MS Homo sapiens
63 CUL3 8452
Affinity Capture-MS Homo sapiens
64 STIM1 6786
Proximity Label-MS Homo sapiens
65 USP54 159195
Two-hybrid Homo sapiens
66 SASS6 163786
Proximity Label-MS Homo sapiens
67 TFG 10342
Two-hybrid Homo sapiens
68 ARFIP1 27236
Co-fractionation Homo sapiens
69 ZNF777  
Affinity Capture-MS Homo sapiens
70 RPA3 6119
Proximity Label-MS Homo sapiens
71 DYNC1LI1 51143
Proximity Label-MS Homo sapiens
72 ATP2A1 487
Proximity Label-MS Homo sapiens
73 SEC16A 9919
Affinity Capture-MS Homo sapiens
74 RAB9A 9367
Co-fractionation Homo sapiens
75 DSTN 11034
Co-fractionation Homo sapiens
76 STAT6 6778
Co-fractionation Homo sapiens
77 THADA 63892
Co-fractionation Homo sapiens
78 UBXN2B  
Affinity Capture-MS Homo sapiens
79 TACC3  
Co-fractionation Homo sapiens
80 STUB1 10273
Co-fractionation Homo sapiens
81 STAT4  
Proximity Label-MS Homo sapiens
82 SEC24B 10427
Negative Genetic Homo sapiens
Co-fractionation Homo sapiens
83 RNF43  
Proximity Label-MS Homo sapiens
84 PEF1 553115
Two-hybrid Homo sapiens
85 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
86 TTC1 7265
Co-fractionation Homo sapiens
87 ROCK1 6093
Co-fractionation Homo sapiens
88 ILF3 3609
Co-fractionation Homo sapiens
89 TP53 7157
Affinity Capture-MS Homo sapiens
90 USP34 9736
Co-fractionation Homo sapiens
91 KIAA0226L  
Affinity Capture-MS Homo sapiens
92 PCMT1 5110
Co-fractionation Homo sapiens
93 SEC23B 10483
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 GFOD1  
Affinity Capture-MS Homo sapiens
95 TRPC3  
Affinity Capture-MS Homo sapiens
96 UBE2C 11065
Co-fractionation Homo sapiens
97 NUP35 129401
Proximity Label-MS Homo sapiens
98 ERGIC1 57222
Proximity Label-MS Homo sapiens
99 TUBB6 84617
Co-fractionation Homo sapiens
100 ERGIC2 51290
Proximity Label-MS Homo sapiens
101 UNK  
Co-fractionation Homo sapiens
102 UBE2B 7320
Co-fractionation Homo sapiens
103 KCNJ10  
Affinity Capture-MS Homo sapiens
104 CYHR1  
Co-fractionation Homo sapiens
105 UPF1 5976
Co-fractionation Homo sapiens
106 SSSCA1 10534
Co-fractionation Homo sapiens
107 SNX2 6643
Co-fractionation Homo sapiens
108 NFIC 4782
Co-fractionation Homo sapiens
109 PSMD1 5707
Co-fractionation Homo sapiens
110 CAV1 857
Proximity Label-MS Homo sapiens
111 PTAR1 375743
Synthetic Lethality Homo sapiens
112 JMJD6 23210
Co-fractionation Homo sapiens
113 DERL1 79139
Proximity Label-MS Homo sapiens
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