Gene description for NAA25
Gene name N(alpha)-acetyltransferase 25, NatB auxiliary subunit
Gene symbol NAA25
Other names/aliases C12orf30
MDM20
NAP1
Species Homo sapiens
 Database cross references - NAA25
ExoCarta ExoCarta_80018
Vesiclepedia VP_80018
Entrez Gene 80018
HGNC 25783
MIM 612755
UniProt Q14CX7  
 NAA25 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NAA25
Molecular Function
    protein binding GO:0005515 IPI
    acetyltransferase activator activity GO:0010698 IBA
Biological Process
    cytoskeleton organization GO:0007010 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    NatB complex GO:0031416 IBA
    NatB complex GO:0031416 IPI
 Experiment description of studies that identified NAA25 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NAA25
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 AP2S1 1175
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 RHOF 54509
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ZBTB2 57621
Affinity Capture-MS Homo sapiens
6 REG1B  
Affinity Capture-MS Homo sapiens
7 CXCR4 7852
Affinity Capture-MS Homo sapiens
8 GPR17 2840
Affinity Capture-MS Homo sapiens
9 PARP9 83666
Co-fractionation Homo sapiens
10 NAA20  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
11 TBC1D9  
Affinity Capture-MS Homo sapiens
12 CHMP4C 92421
Affinity Capture-MS Homo sapiens
13 PSMB7 5695
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 TNFRSF8 943
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ABL1 25
Co-fractionation Homo sapiens
16 SMAD4  
Affinity Capture-MS Homo sapiens
17 EGFR 1956
Negative Genetic Homo sapiens
18 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
19 MILR1  
Affinity Capture-MS Homo sapiens
20 PRKAR2B 5577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 APP 351
Reconstituted Complex Homo sapiens
22 CDK2 1017
Affinity Capture-MS Homo sapiens
23 ARHGAP44  
Affinity Capture-MS Homo sapiens
24 FGFR1OP2  
Affinity Capture-MS Homo sapiens
25 BBS1 582
Affinity Capture-MS Homo sapiens
26 PAK4 10298
Co-fractionation Homo sapiens
27 NTRK1 4914
Affinity Capture-MS Homo sapiens
28 MND1  
Affinity Capture-MS Homo sapiens
29 AHR 196
Affinity Capture-MS Homo sapiens
30 SPRTN  
Affinity Capture-MS Homo sapiens
31 CENPM  
Affinity Capture-MS Homo sapiens
32 NRBF2  
Affinity Capture-MS Homo sapiens
33 LUC7L2 51631
Affinity Capture-MS Homo sapiens
34 BRD4 23476
Affinity Capture-MS Homo sapiens
35 RPA3 6119
Proximity Label-MS Homo sapiens
36 AKAP1 8165
Affinity Capture-MS Homo sapiens
37 SUZ12  
Affinity Capture-MS Homo sapiens
38 REG1A  
Affinity Capture-MS Homo sapiens
39 CACYBP 27101
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which NAA25 is involved
No pathways found





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