Gene description for PAK6
Gene name p21 protein (Cdc42/Rac)-activated kinase 6
Gene symbol PAK6
Other names/aliases PAK5
Species Homo sapiens
 Database cross references - PAK6
ExoCarta ExoCarta_56924
Entrez Gene 56924
HGNC 16061
MIM 608110
UniProt Q9NQU5  
 PAK6 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocytes 26054723    
 Gene ontology annotations for PAK6
Molecular Function
    Rac GTPase binding GO:0048365 IBA
    protein binding GO:0005515 IPI
    protein serine/threonine kinase activity GO:0004674 IBA
    ATP binding GO:0005524 IEA
Biological Process
    regulation of transcription, DNA-templated GO:0006355 TAS
    regulation of apoptotic process GO:0042981 IBA
    apoptotic process GO:0006915 TAS
    stress-activated protein kinase signaling cascade GO:0031098 IBA
    regulation of mitotic cell cycle GO:0007346 IBA
    cytoskeleton organization GO:0007010 TAS
    Rho protein signal transduction GO:0007266 IBA
    signal transduction by protein phosphorylation GO:0023014 IBA
    cell migration GO:0016477 IBA
    regulation of MAPK cascade GO:0043408 IBA
    actin cytoskeleton organization GO:0030036 IBA
    activation of protein kinase activity GO:0032147 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    nucleus GO:0005634 IEA
 Experiment description of studies that identified PAK6 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for PAK6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAQ 10971
Affinity Capture-MS Homo sapiens
2 AR 367
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 ESR1  
Reconstituted Complex Homo sapiens
4 CDC42 998
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
5 AKT1 207
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which PAK6 is involved
PathwayEvidenceSource
Activation of Rac TAS Reactome





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