Gene description for GUSB
Gene name glucuronidase, beta
Gene symbol GUSB
Other names/aliases BG
MPS7
Species Homo sapiens
 Database cross references - GUSB
ExoCarta ExoCarta_2990
Vesiclepedia VP_2990
Entrez Gene 2990
HGNC 4696
MIM 611499
UniProt P08236  
 GUSB identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 15326289    
Urine 19056867    
Urine 25452312    
 Gene ontology annotations for GUSB
Molecular Function
    beta-glucuronidase activity GO:0004566 IBA
    beta-glucuronidase activity GO:0004566 TAS
    signaling receptor binding GO:0005102 IBA
    signaling receptor binding GO:0005102 IPI
    protein domain specific binding GO:0019904 IPI
    carbohydrate binding GO:0030246 IBA
Biological Process
    carbohydrate metabolic process GO:0005975 IEA
    glycosaminoglycan catabolic process GO:0006027 TAS
    heparan sulfate proteoglycan catabolic process GO:0030200 IEA
    chondroitin sulfate catabolic process GO:0030207 IEA
    hyaluronan catabolic process GO:0030214 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    membrane GO:0016020 HDA
    azurophil granule lumen GO:0035578 TAS
    lysosomal lumen GO:0043202 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    extracellular exosome GO:0070062 HDA
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified GUSB in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
8
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
9
Experiment ID 238
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25452312    
Organism Homo sapiens
Experiment description N-linked (N-) glycoproteomics of urimary exosomes
Authors "Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R."
Journal name Mol Cell Proteomics
Publication year 2014
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GUSB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 ATG9A 79065
Proximity Label-MS Homo sapiens
3 LY6H  
Affinity Capture-MS Homo sapiens
4 GPIHBP1  
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 BTNL2  
Affinity Capture-MS Homo sapiens
7 LILRA5  
Affinity Capture-MS Homo sapiens
8 BCAN  
Affinity Capture-MS Homo sapiens
9 KLK15  
Affinity Capture-MS Homo sapiens
10 CEACAM8 1088
Affinity Capture-MS Homo sapiens
11 CPOX 1371
Co-fractionation Homo sapiens
12 UGT1A10  
Affinity Capture-MS Homo sapiens
13 TMEM87A 25963
Affinity Capture-MS Homo sapiens
14 ALPPL2 251
Affinity Capture-MS Homo sapiens
15 TCTN1  
Affinity Capture-MS Homo sapiens
16 DPEP2  
Affinity Capture-MS Homo sapiens
17 PDIA4 9601
Proximity Label-MS Homo sapiens
18 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 GIT2 9815
Two-hybrid Homo sapiens
21 GGH 8836
Affinity Capture-MS Homo sapiens
22 TRGV3  
Affinity Capture-MS Homo sapiens
23 PDGFRA 5156
Affinity Capture-MS Homo sapiens
24 PRG2  
Affinity Capture-MS Homo sapiens
25 MFAP4 4239
Affinity Capture-MS Homo sapiens
26 ECEL1  
Affinity Capture-MS Homo sapiens
27 RLN1  
Affinity Capture-MS Homo sapiens
28 CBLN4  
Affinity Capture-MS Homo sapiens
29 LY9 4063
Affinity Capture-MS Homo sapiens
30 FAHD1 81889
Co-fractionation Homo sapiens
31 LYZL1  
Affinity Capture-MS Homo sapiens
32 FBXO6 26270
Affinity Capture-MS Homo sapiens
33 SLURP1  
Affinity Capture-MS Homo sapiens
34 NCR3  
Affinity Capture-MS Homo sapiens
35 IL5RA  
Affinity Capture-MS Homo sapiens
36 ELSPBP1  
Affinity Capture-MS Homo sapiens
37 HOXA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SH2D3A  
Affinity Capture-MS Homo sapiens
39 VRK1 7443
Affinity Capture-MS Homo sapiens
40 IGLL5 100423062
Affinity Capture-MS Homo sapiens
41 C1QB 713
Affinity Capture-MS Homo sapiens
42 SIRPD  
Affinity Capture-MS Homo sapiens
43 IDS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 SDF2L1 23753
Affinity Capture-MS Homo sapiens
45 FIBIN  
Affinity Capture-MS Homo sapiens
46 CDK4 1019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 FBXO2 26232
Affinity Capture-MS Homo sapiens
48 SCGB2A2  
Affinity Capture-MS Homo sapiens
49 C1orf54  
Affinity Capture-MS Homo sapiens
50 ADAMTS4  
Affinity Capture-MS Homo sapiens
51 ALPP 250
Affinity Capture-MS Homo sapiens
52 C1QTNF9B  
Affinity Capture-MS Homo sapiens
53 EPHA2 1969
Proximity Label-MS Homo sapiens
54 CDHR4  
Affinity Capture-MS Homo sapiens
55 DNAJC3 5611
Proximity Label-MS Homo sapiens
56 EDN3  
Affinity Capture-MS Homo sapiens
57 NXPH3  
Affinity Capture-MS Homo sapiens
58 ISLR 3671
Affinity Capture-MS Homo sapiens
59 ZBTB42  
Affinity Capture-MS Homo sapiens
60 CFC1  
Affinity Capture-MS Homo sapiens
61 PMEL 6490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 CES1  
Affinity Capture-Western Homo sapiens
63 TMEM192 201931
Affinity Capture-MS Homo sapiens
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