Gene description for HACD3
Gene name 3-hydroxyacyl-CoA dehydratase 3
Gene symbol HACD3
Other names/aliases B-IND1
BIND1
HSPC121
PTPLAD1
Species Homo sapiens
 Database cross references - HACD3
ExoCarta ExoCarta_51495
Vesiclepedia VP_51495
Entrez Gene 51495
HGNC 24175
MIM 615940
UniProt Q9P035  
 HACD3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for HACD3
Molecular Function
    GTPase activator activity GO:0005096 TAS
    protein binding GO:0005515 IPI
    3-hydroxyacyl-CoA dehydratase activity GO:0018812 IBA
    enzyme binding GO:0019899 IDA
    very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity GO:0102158 IDA
Biological Process
    canonical NF-kappaB signal transduction GO:0007249 TAS
    JNK cascade GO:0007254 IEA
    small GTPase-mediated signal transduction GO:0007264 TAS
    Rho protein signal transduction GO:0007266 IEA
    Rac protein signal transduction GO:0016601 NAS
    sphingolipid biosynthetic process GO:0030148 IBA
    fatty acid elongation GO:0030497 IBA
    fatty acid elongation GO:0030497 IDA
    very long-chain fatty acid biosynthetic process GO:0042761 IBA
    very long-chain fatty acid biosynthetic process GO:0042761 IDA
    positive regulation of viral genome replication GO:0045070 IMP
    positive regulation by virus of viral protein levels in host cell GO:0046726 IMP
    negative regulation of intracellular signal transduction GO:1902532 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IBA
    focal adhesion GO:0005925 HDA
    nuclear membrane GO:0031965 IDA
 Experiment description of studies that identified HACD3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HACD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 EIF3A 8661
Cross-Linking-MS (XL-MS) Homo sapiens
3 SLC25A13 10165
Co-fractionation Homo sapiens
4 HSD17B11 51170
Proximity Label-MS Homo sapiens
5 ISG15 9636
Affinity Capture-MS Homo sapiens
6 SPRTN  
Affinity Capture-MS Homo sapiens
7 LGR4 55366
Affinity Capture-MS Homo sapiens
8 PPP5C 5536
Affinity Capture-MS Homo sapiens
9 VAPA 9218
Affinity Capture-MS Homo sapiens
10 RIN3  
Affinity Capture-MS Homo sapiens
11 BZW2 28969
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
12 SERINC3 10955
Affinity Capture-MS Homo sapiens
13 HSPA1L 3305
Co-fractionation Homo sapiens
14 PLD4  
Affinity Capture-MS Homo sapiens
15 GJD3  
Proximity Label-MS Homo sapiens
16 KARS 3735
Co-fractionation Homo sapiens
17 CAND1 55832
Affinity Capture-MS Homo sapiens
18 KIAA1429 25962
Affinity Capture-MS Homo sapiens
19 CORO1B 57175
Co-fractionation Homo sapiens
20 FBXW7  
Affinity Capture-MS Homo sapiens
21 UBL4A 8266
Affinity Capture-MS Homo sapiens
22 SSR1 6745
Proximity Label-MS Homo sapiens
23 OBSL1 23363
Affinity Capture-MS Homo sapiens
24 DDOST 1650
Affinity Capture-MS Homo sapiens
25 PLD2 5338
Affinity Capture-MS Homo sapiens
26 FANCD2  
Affinity Capture-MS Homo sapiens
27 C20orf24  
Affinity Capture-MS Homo sapiens
28 ARHGAP19  
Affinity Capture-MS Homo sapiens
29 CCDC18  
Cross-Linking-MS (XL-MS) Homo sapiens
30 ATG16L1 55054
Affinity Capture-MS Homo sapiens
31 BRF2  
Affinity Capture-MS Homo sapiens
32 HSD17B4 3295
Co-fractionation Homo sapiens
33 KLF15  
Affinity Capture-MS Homo sapiens
34 FAF2 23197
Co-fractionation Homo sapiens
35 LMAN1 3998
Proximity Label-MS Homo sapiens
36 KLF16  
Affinity Capture-MS Homo sapiens
37 EPB41L3 23136
Cross-Linking-MS (XL-MS) Homo sapiens
38 VAV1 7409
Affinity Capture-MS Homo sapiens
39 ALDH16A1 126133
Co-fractionation Homo sapiens
40 PELO 53918
Affinity Capture-MS Homo sapiens
41 RB1CC1 9821
Affinity Capture-MS Homo sapiens
42 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 STAU1 6780
Affinity Capture-MS Homo sapiens
44 ACACA 31
Negative Genetic Homo sapiens
45 Vav2  
Affinity Capture-MS Mus musculus
46 ESRRB  
Affinity Capture-MS Homo sapiens
47 PMAIP1  
Affinity Capture-MS Homo sapiens
48 NTRK1 4914
Affinity Capture-MS Homo sapiens
49 SCCPDH 51097
Co-fractionation Homo sapiens
50 NOS2  
Affinity Capture-MS Homo sapiens
51 RPN1 6184
Proximity Label-MS Homo sapiens
52 CHCHD6 84303
Co-fractionation Homo sapiens
53 HECTD1 25831
Affinity Capture-MS Homo sapiens
54 SEC62 7095
Proximity Label-MS Homo sapiens
55 ELOVL5 60481
Proximity Label-MS Homo sapiens
56 REEP5 7905
Proximity Label-MS Homo sapiens
57 C16orf72 29035
Affinity Capture-MS Homo sapiens
58 SEC63 11231
Affinity Capture-MS Homo sapiens
59 TTYH1  
Affinity Capture-MS Homo sapiens
60 CANX 821
Affinity Capture-MS Homo sapiens
61 LRRC59 55379
Co-fractionation Homo sapiens
62 RC3H2  
Affinity Capture-MS Homo sapiens
63 ATP2B1 490
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
64 LAMP1 3916
Proximity Label-MS Homo sapiens
65 BCAP31 10134
Affinity Capture-MS Homo sapiens
66 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 TMEM258  
Affinity Capture-MS Homo sapiens
68 ARHGEF16 27237
Affinity Capture-MS Homo sapiens
69 EPRS 2058
Co-fractionation Homo sapiens
70 CORO1C 23603
Co-fractionation Homo sapiens
71 MYO1C 4641
Co-fractionation Homo sapiens
72 OCIAD1 54940
Proximity Label-MS Homo sapiens
73 AURKB 9212
Affinity Capture-MS Homo sapiens
74 FASN 2194
Negative Genetic Homo sapiens
75 FKBP5 2289
Affinity Capture-MS Homo sapiens
76 ARHGAP25  
Affinity Capture-MS Homo sapiens
77 ARHGAP15 55843
Affinity Capture-MS Homo sapiens
78 CNR2  
Affinity Capture-MS Homo sapiens
79 HECW2  
Affinity Capture-MS Homo sapiens
80 ATP2A1 487
Proximity Label-MS Homo sapiens
81 EMC2 9694
Co-fractionation Homo sapiens
82 RAB9A 9367
Proximity Label-MS Homo sapiens
83 DDRGK1 65992
Affinity Capture-MS Homo sapiens
84 BMI1  
Affinity Capture-MS Homo sapiens
85 AARS 16
Cross-Linking-MS (XL-MS) Homo sapiens
86 ARHGAP31 57514
Affinity Capture-MS Homo sapiens
87 PARP16 54956
Affinity Capture-MS Homo sapiens
88 DOCK3  
Affinity Capture-MS Homo sapiens
89 HUWE1 10075
Affinity Capture-MS Homo sapiens
90 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
91 PHB2 11331
Co-fractionation Homo sapiens
92 TMED10 10972
Affinity Capture-MS Homo sapiens
93 PARP1 142
Co-fractionation Homo sapiens
94 ILF3 3609
Co-fractionation Homo sapiens
95 CKAP4 10970
Affinity Capture-MS Homo sapiens
96 FBXL6  
Affinity Capture-MS Homo sapiens
97 TP53 7157
Affinity Capture-MS Homo sapiens
98 PGRMC1 10857
Affinity Capture-MS Homo sapiens
99 NEK6 10783
Affinity Capture-MS Homo sapiens
100 PEX19 5824
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
101 TPTE  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
102 EMC4 51234
Co-fractionation Homo sapiens
103 GABARAPL2 11345
Affinity Capture-MS Homo sapiens
104 TSPAN17 26262
Affinity Capture-MS Homo sapiens
105 Arhgap30  
Affinity Capture-MS Mus musculus
106 INSR 3643
Affinity Capture-Western Homo sapiens
107 Rac1 19353
Phenotypic Enhancement Mus musculus
Affinity Capture-Western Mus musculus
108 FILIP1  
Cross-Linking-MS (XL-MS) Homo sapiens
109 GNB1 2782
Affinity Capture-MS Homo sapiens
110 ATP2A2 488
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
111 ESR1  
Affinity Capture-MS Homo sapiens
112 UFL1 23376
Affinity Capture-MS Homo sapiens
113 PLD3 23646
Affinity Capture-MS Homo sapiens
114 ARHGAP36  
Affinity Capture-MS Homo sapiens
115 SPPL2B 56928
Affinity Capture-MS Homo sapiens
116 XRCC6 2547
Co-fractionation Homo sapiens
117 Setd2  
Affinity Capture-MS Mus musculus
118 CUL7 9820
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 C9orf72  
Affinity Capture-MS Homo sapiens
120 FANCI 55215
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
121 ATXN3 4287
Affinity Capture-MS Homo sapiens
122 PPP2R2A 5520
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
123 PLD6  
Affinity Capture-MS Homo sapiens
124 RPA3 6119
Proximity Label-MS Homo sapiens
125 EP300 2033
Affinity Capture-MS Homo sapiens
126 DERL1 79139
Proximity Label-MS Homo sapiens
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